Decreased Sperm Motility in Rats Orally Exposed to Single or Mixed Pesticides

Perobelli, Juliana Elaine; Martinez, Marcelo; Franchi, Carla Adriene da Silva; Fernandez, Carla Dal Bianco; Camargo, João Lauro Viana de; Kempinas, Wilma De Grava

doi:10.1080/15287391003751802

Cited by 64 publications

(28 citation statements)

References 49 publications

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“…Spermatozoa were classified as: mobile with progressive movement, mobile without progressive movement or immobile. This analysis was performed as described by Perobelli and colleagues [28]. …”

Section: Methodsmentioning

confidence: 99%

Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats

Fernandes

Fernandez

Campos

et al. 2011

Reprod Biol Endocrinol

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BackgroundHyperglycemia can impair the male reproductive system in experimental animals and in men during reproductive age. Studies have shown that vitamin C has some good effects on male reproductive system, and therefore vitamin C treatment could attenuate the dysfunctions in this system caused by hyperglycemia. Thus, the objective of this work was to evaluate whether vitamin C treatment could attenuate reproductive dysfunctions in hyperglycemic male rats.MethodsAdult male rats were divided into 3 groups: a normoglycemic (n = 10) and two hyperglycemic (that received a single dose of streptozotocin - 40 mg/kg BW). The two last groups (n = 10 per group) were divided into: hyperglycemic control (Hy) and hyperglycemic + 150 mg of vitamin C (HyC), by gavage during 30 consecutive days. The normoglycemic and hyperglycemic control groups received the vehicle (water). The first day after the treatment, the rats were anesthetized and killed to evaluate oxidative stress biomarkers (TBARS, SOD, GSHt and GSH-Px) in the erythrocytes, body and reproductive organ weights, sperm parameters, plasma hormone levels (FSH, LH and testosterone), testicular and epididymal histo-morphometry and histopathology.ResultsCompared with the normoglycemic animals, hyperglycemic control rats showed reduced weight of the body and reproductive organ but testis weight was maintained. It was also observed reduction of testosterone and LH levels, seminiferous tubular diameter, sperm motility and sperm counts in the epididymis. In addition, there was an increase in morphological abnormalities on spermatozoa as well as in oxidative stress level. Vitamin C reduced the oxidative stress level, diminished the number of abnormal sperm, and increased testosterone and LH levels and seminiferous tubular diameter but did not show improvement of sperm motility in relation to the hyperglycemic control group. Hyperglycemia caused a rearrangement in the epididymal tissue components (stroma, ephitelium and lumen) as demonstrated by the stereological analysis results. However, this alteration was partially prevented by vitamin C treatment.ConclusionsWe conclude that vitamin C partially attenuated some male reproductive system dysfunctions in hyperglycemic rats.

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Section: Methodsmentioning

confidence: 99%

Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats

Fernandes

Fernandez

Campos

et al. 2011

Reprod Biol Endocrinol

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“…Sperm motility was analyzed as described by Perobelli et al (2010) on spermatozoa collected from the right vas deferens.…”

Section: Methodsmentioning

confidence: 99%

Acceleration of Sperm Transit Time and Reduction of Sperm Reserves in the Epididymis of Rats Exposed to Sibutramine

Bellentani

Fernandes

Perobelli

et al. 2011

Journal of Andrology

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Sibutramine is a drug globally used for the treatment of obesity. The aim of this study was to investigate male reproductive disorders caused by sibutramine in adult rats. Wistar rats were treated for 28 consecutive days (gavage) with 10 mg/kg of sibutramine. Control animals received only vehicle (dimethylsulfoxide and saline). The rats were sacrificed for evaluation of body and reproductive organ weights, sperm parameters, hormone levels (luteinizing hormone, follicle-stimulating hormone, and testosterone), testicular and epididymal histopathology, sexual behavior, fertility and in vitro contractility of the epididymal duct. Sibutramine decreased (P , .05) weights of the epididymis and ventral prostate, but not of other reproductive organs. The sperm number and transit time in the epididymal cauda were decreased (P , .001), but the daily sperm production was not altered. Moreover, morphology and sperm motility, histopathology of the testes and epididymis, sexual behavior, fertility, and serum hormone levels were not altered by the treatment. Sibutramine increased the potency of norepinephrine and, per se, increased the mechanical activity of the epididymal duct in vitro. Thus, although sibutramine in these experimental conditions did not interfere with the reproductive process of rats, it provoked acceleration of the sperm transit time and a decrease in the sperm reserves in the epididymal cauda. This alteration is probably related to the sympathomimetic effect of this drug, as shown by the in vitro assays. In humans, use of this drug might present a threat for male fertility because sperm reserves in men are naturally lower than those in rats.

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“…Spermatogenesis and sperm function are known to be sensitive to environmental stress including exposure to pesticides . Previous studies demonstrated that steroidogenesis, sperm motility and sperm production was decreased in rats exposed to a mixture of pesticides . Decreased gene expression observed in this study could be due to the ability of allethrin to influence gene transcription machinery, which may involve complex signaling pathways.…”

Section: Discussionmentioning

confidence: 67%

Allethrin toxicity causes reproductive dysfunction in male rats

Madhubabu

Yenugu

2017

Environmental Toxicology

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Pyrethroids are widely used for domestic and agricultural purposes and their use is increasing, especially in developing countries. Uncontrolled use of these insecticides resulted in their entry into the food chain thereby causing toxicity to different organ systems. Allethrin is one of the widely used pyrethroids, but its toxicological effects are underreported when compared to other pyrethroids. Further, its effects on the male reproductive tract remain uncharacterized. In this study, its toxicity on the male reproductive tract was evaluated by administering 25-150 mg/kg body weight allethrin to adult rats for 60 days. The mRNA expression of factors that are important in spermatogenesis (Scf, c-Kit, Hsf2, Ovol1, Brdt, Kdm3A, Ybx-2, and Grth) and steroidogenesis (StAR, 3β-HSD, 17β-HSD) was significantly downregulated. Decreased levels of testosterone, reduced sperm count and daily sperm production was also observed due to allethrin toxicity. However, sperm quality parameters assessed by computer-assisted sperm analyzer were not affected. Spermatozoa obtained from allethrin-treated rats failed to undergo acrosome reaction. Results of this study indicate that allethrin affects spermatogenesis and sperm function, thus lending further support to the growing evidence of its toxicity.

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Decreased Sperm Motility in Rats Orally Exposed to Single or Mixed Pesticides

Cited by 64 publications

References 49 publications

Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats

Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats

Acceleration of Sperm Transit Time and Reduction of Sperm Reserves in the Epididymis of Rats Exposed to Sibutramine

Allethrin toxicity causes reproductive dysfunction in male rats

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