Decreased Spp1 Expression in Acute Myocardial Infarction after Ischemia and Reperfusion Injury

Abstract

Background. With the progress of shock therapy and the establishment and promotion of methods such as thrombolytic therapy and percutaneous coronary intervention (PCI), many tissues and organs have been reperfused after ischemia which may cause even worse disorder called ischemia-reperfusion injury (IRI). mRNAs have been found to have significant impacts on ischemia-reperfusion through various mechanisms. In view of the accessibility of mRNAs from blood, we aimed to find the association between mRNA and ischemia-reperfusion. Methods. We used the GSE83472 dataset from the Gene Expression Omnibus (GEO) database to find differential RNA expression between ischemia-reperfusion tissue and control samples. In addition, Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to find the biological property of 449 RNAs from GSE83472 via the Database for Annotation, Visualization, and Integrated Discovery (DAVID). Besides, Gene Set Enrichment Analysis (GSEA), a tool to find the pathway orientation of a gene set, was used for further study in the four most significant KEGG pathways. Furthermore, we constructed a protein-protein interaction (PPI) network. In the end, we used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting to measure and compare the expression of Spp1 in patients who accepted percutaneous coronary intervention. Results. The bioinformatics analyses suggested that Spp1 was a hub gene in reperfusion after ischemia. The qRT-PCR result showed that the Spp1 expression was significantly downregulated in ischemia-reperfusion cells after PCI compared with normal samples and so as the western blotting. Conclusion. Spp1 might play an essential role in acute myocardial infarction after ischemia and reperfusion injury.

The Mechanisms of Ferroptosis Under Hypoxia

“…MIRI refers to the cardiac function damage resulted from recovery of blood supply in a short time after partial or total myocardial coronary artery occlusion, which often occurs in acute myocardial infarction, coronary heart disease (Abudunaibi et al 2015 ; Dong et al 2019 ; Li et al 2021b ).…”

Identification of key genes and mechanisms of epicardial adipose tissue in patients with diabetes through bioinformatic analysis

“…Its encoded protein is also a cytokine that upregulates the expression of IFN-γ and IL-12. SPP1 may play an important role in acute myocardial infarction after ischemia and reperfusion injury (49). Moreover, SPP1 promotes human cardiac fibroblast fibrosis by reducing p27 expression through the regulation of the PI3K/Akt signaling pathway (50).…”

Retracted: Decreased Spp1 Expression in Acute Myocardial Infarction after Ischemia and Reperfusion Injury

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Peer-review manipulation …”

“…Tis article has been retracted by Hindawi following an investigation undertaken by the publisher [1]. Tis investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process:…”