Dectin-1 is required for miR155 upregulation in murine macrophages in response to<i>Candida albicans</i>

Agustinho, Daniel Paiva; Oliveira, Marco Antônio de; Tavares, Aldo Henrique; Derengowski, Lorena da Silveira; Stolz, Valentina; Guilhelmelli, Fernanda; Mortari, Márcia Renata; Kuchler, Karl; Silva-Pereira, Ildinete

doi:10.1080/21505594.2016.1200215

Cited by 19 publications

(21 citation statements)

References 70 publications

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“…Therefore, it is reasonable to hypothesize that receptor binding on the cell surface may be one of the pathways by which PH-EPS acts on macrophages. To recognize various components on the fungal cell wall, macrophages express PRRs on their surface through which pathogen-associated molecular patterns can be recognized (42). Recent studies have demonstrated that PRRs such as TLR4, TLR2, CR3 and Dectin-1 have significant roles in macrophage activation when being exposed to polysaccharides (14,43–45).…”

Section: Resultsmentioning

confidence: 99%

Exopolysaccharide from Paecilomyces lilacinus modulates macrophage activities through the TLR4/NF‑κB/MAPK pathway

Lin

Wang

et al. 2019

Mol Med Report

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Multiple exopolysaccharides (EPSs) have been isolated from various organisms in extreme environments and have yielded a variety of activities. The present study evaluated the immunomodulatory capabilities of an EPS (termed PH-EPS) derived from the fungus Paecilomyces lilacinus PH0016, which was isolated from a tropical and hyperhaline environment in southern China. The macrophage RAW 264.7 cell line was used to investigate the mechanism of PH-EPS-induced macrophage activation. The results indicated that RAW 264.7 macrophages were activated by PH-EPS, in an effect slightly inferior to lipopolysaccharide (LPS), as evidenced by secretion of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and nitric oxide (NO), and by significantly increased phagocytosis in the cells treated with PH-EPS. Nuclear factor (NF)-κB p65 was significantly translocated into the nucleus in the PH-EPS-treated cells. In addition, expression of inducible NO synthase (iNOS) and IκB-α degradation were enhanced in PH-EPS-treated cells. The phosphorylation levels of p38, JNK and ERK were also significantly increased in the PH-EPS-treated cells. Furthermore, IL-1β and TNF-α production was markedly decreased in PH-EPS-treated cells when the mitogen-activated protein kinase (MAPK) pathways were blocked by the inhibitor Dectin-1 and by antibodies against Toll-like receptor 4 (TLR4). The present results indicated that PH-EPS from Paecilomyces lilacinus possessed the capability of activating RAW 264.7 cells via the TLR4/NF-κB/MAPKs signaling pathway.

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Section: Resultsmentioning

confidence: 99%

Exopolysaccharide from Paecilomyces lilacinus modulates macrophage activities through the TLR4/NF‑κB/MAPK pathway

Lin

Wang

et al. 2019

Mol Med Report

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“…miR‐155 is known as one of the master regulatory miRNAs in the immunological processes. Agustinho et al revealed that miR‐155 induction by C. albicans hyphae was fully dependent on dectin‐1 which exerted the Syk‐dependent pathway . They also identified reduced mRNA expression of some of the miR‐155 targets, such as MyD88, SHIP‐1, SOCS1 and IKKe that was occurred soon after miR‐155 detection.…”

Section: Mirnas Profiles Following Fungal Exposurementioning

confidence: 99%

Interactions between immune response to fungal infection and microRNAs: The pioneer tuners

Pakshir

Badali

Nami

et al. 2019

Mycoses

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Summary Due to their physiological and biological characteristics, numerous fungi are potentially emerging pathogens. Active dynamicity of fungal pathogens causes life‐threatening infections annually impose high costs to the health systems. Although immune responses play crucial roles in controlling the fate of fungal infections, immunocompromised patients are at high risk with high mortality. Tuning the immune response against fungal infections might be an effective strategy for controlling and reducing the pathological damages. MicroRNAs (miRNAs) are known as the master regulators of immune response. These single‐stranded tuners (18‐23 bp non‐coding RNAs) are endogenously expressed by all metazoan eukaryotes and have emerged as the master gene expression controllers of at least 30% human genes. In this review article, following the review of biology and physiology (biogenesis and mechanism of actions) of miRNAs and immune response against fungal infections, the interactions between them were scrutinised. In conclusion, miRNAs might be considered as one of the potential goals in immunotherapy for fungal infections. Undoubtedly, advanced studies in this field, further identifying of miRNA roles in governing the immune response, pave the way for inclusion of miRNA‐related immunotherapeutic in the treatment of life‐threatening fungal infections.

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“…In a murine model of subchronic A. fumigatus inhalation exposure, Croston et al showed that significantly downregulated miR-29a-3p was predicted to regulate C-type lectin domain family 7 member A, the gene that codes for Dectin-1 ( 91 ). A recent study found that following exposure to C. albicans , Dectin-1 is required for the upregulation of miR-155 in murine macrophages ( 93 ). Along with an increase in miR-30-5p and miR-210-3p in THP-1 cells treated with β-glucan isolated from C. albicans , Du et al found that miR-146a was increased upon Dectin-1 stimulation and negatively regulated the resultant inflammatory response ( 94 ).…”

Section: Fungal Exposure: Role Of Mirnasmentioning

confidence: 99%

MicroRNA Regulation of Host Immune Responses following Fungal Exposure

et al. 2018

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Fungal bioaerosols are ubiquitous in the environment and human exposure can result in a variety of health effects ranging from systemic, subcutaneous, and cutaneous infections to respiratory morbidity including allergy, asthma, and hypersensitivity pneumonitis. Recent research has focused on the role of microRNAs (miRNAs) following fungal exposure and is overlooked, yet important, group of regulators capable of influencing fungal immune responses through a variety of cellular mechanisms. These small non-coding ribose nucleic acids function to regulate gene expression at the post-transcriptional level and have been shown to participate in multiple disease pathways including cancer, heart disease, apoptosis, as well as immune responses to microbial hazards and occupational allergens. Recent animal model studies have characterized miRNAs following the exposure to inflammatory stimuli. Studies focused on microbial exposure, including bacterial infections, as well as exposure to different allergens have shown miRNAs, such as miR-21, miR-146, miR-132, miR-155, and the let-7 family members, to be involved in immune and inflammatory responses. Interestingly, the few studies have assessed that the miRNA profiles following fungal exposure have identified the same critical miRNAs that have been characterized in other inflammatory-mediated and allergy-induced experimental models. Review of available in vitro, animal and human studies of exposures to Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Paracoccidioides brasiliensis, and Stachybotrys chartarum identified several miRNAs that were shared between responses to these species including miR-125 a/b (macrophage polarization/activation), miR-132 [toll-like receptor (TLR)2-mediated signaling], miR-146a (TLR mediated signaling, alternative macrophage activation), and miR-29a/b (natural killer cell function, C-leptin signaling, inhibition of Th1 immune response). Although these datasets provide preliminary insight into the role of miRNAs in fungal exposed models, interpretation of miRNA datasets can be challenging for researchers. To assist in navigating this rapidly evolving field, the aim of this review is to describe miRNAs in the framework of host recognition mechanisms and provide initial insight into the regulatory pathways in response to fungal exposure.

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Dectin-1 is required for miR155 upregulation in murine macrophages in response toCandida albicans

Cited by 19 publications

References 70 publications

Exopolysaccharide from Paecilomyces lilacinus modulates macrophage activities through the TLR4/NF‑κB/MAPK pathway

Exopolysaccharide from Paecilomyces lilacinus modulates macrophage activities through the TLR4/NF‑κB/MAPK pathway

Interactions between immune response to fungal infection and microRNAs: The pioneer tuners

MicroRNA Regulation of Host Immune Responses following Fungal Exposure

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