Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode

Modeling the competitive adsorption of sample solvent and solute in supercritical fluid chromatography

“…Supercritical fluid chromatography (SFC) has gathered increased attention over the last few years in the fields of both analytical [1,2] and preparative chromatography [3][4][5][6], theoretical research (e.g. the effects of sample diluent [7], mobile phase density [8], modifier adsorption [9,10]), as well as forensics [11], chiral separations [12] and numerous other areas of application including the pharmaceutical [13] and food industry [14]. Its growing reputation can be associated with the rapid technological advancements of the last decade that make SFC a highly viable and comparable, but ultimately a complementary technique besides liquid chromatography (LC).…”

Enantioseparation and determination of flumequine enantiomers in multiple food matrices with chiral liquid chromatography coupled with tandem mass spectrometry

“…The selection of chiral column with different stationary phases is a key factor to achieve efficient enantioseparation. Among the various CSPs, the polysaccharide‐based CSPs are extensively used, which show good chiral recognition ability toward many chiral compounds …”

“…Among the various CSPs, the polysaccharide-based CSPs are extensively used, which show good chiral recognition ability toward many chiral compounds. [26][27][28][29][30] In the preliminary experiments, the enantioseparation of flumequine on four cellulose-based columns (Chiralpak IB and Chiralpak IC, Chiralcel OD-RH and Chiralcel OJ-RH) and two amylose-based chiral columns (Chiralpak IA and Chiralpak ID) was tested using a variety of reversed-phase mobile phase combinations. The initial column screening was conducted under the same mobile condition, with a organic solvent of 80% methanol or 60% acetonitrile at a flow rate of 0.6 mL min -1 .…”

Liquid chromatographic resolution of natural and racemic Cinchona alkaloid analogues using strong cation- and zwitterion ion-exchange type stationary phases. Qualitative evaluation of stationary phase characteristics and mobile phase effects on stereoselectivity and retention

“…As it has been investigated earlier, chiral ion-exchangers can in principle be operated in hydro-organic, in polar organic, and in SFC modes using liquid CO 2 with adapted bulk solvent compositions, but always containing appropriate amounts of organic salts in the mobile phase acting as counter-ions [29] . Based on these information, the two ZWIX( + ) and ZWIX( −) columns have been investigated in SFC modality using liquid CO 2 mixed with various amounts of MeOH.…”