Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture

Schnabel, M.; Marlovits, Stefan; Eckhoff, G.; Fichtel, I.; Götzen, L.; Vécsei, V.; Schlegel, Jürgen

doi:10.1053/joca.2001.0482

Cited by 521 publications

(384 citation statements)

References 24 publications

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“…Additionally, MSC can be expanded extensively in vitro whilst chondrocytes hold a limited culture growth capacity and display phenotypic instability during the course of their expansion in monolayer culture. Such phenotypic instability (also called ''dedifferentiation'') is characterised by a shift of cellular morphology from a rounded to the typical fusiform fibroblastic shape, among other features (Schnabel et al 2002), as we also observed in the present study (Fig. 1i).…”

Section: Discussionsupporting

confidence: 85%

Cartilage resurfacing potential of PLGA scaffolds loaded with autologous cells from cartilage, fat, and bone marrow in an ovine model of osteochondral focal defect

et al. 2015

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Current developments in tissue engineering strategies for articular cartilage regeneration focus on the design of supportive three-dimensional scaffolds and their use in combination with cells from different sources. The challenge of translating initial successes in small laboratory animals into the clinics involves pilot studies in large animal models, where safety and efficacy should be investigated during prolonged follow-up periods. Here we present, in a single study, the long-term (up to 1 year) effect of biocompatible porous scaffolds non-seeded and seeded with fresh ex vivo expanded autologous progenitor cells that were derived from three different cell sources [cartilage, fat and bone marrow (BM)] in order to evaluate their advantages as cartilage resurfacing agents. An ovine model of critical size osteochondral focal defect was used and the test items were implanted arthroscopically into the knees. Evidence of regeneration of hyaline quality tissue was observed at 6 and 12 months post-treatment with variable success depending on the cell source. Cartilage and BM-derived mesenchymal stromal cells (MSC), but not those derived from fat, resulted in the best quality of new cartilage, as judged qualitatively by magnetic resonance imaging and macroscopic assessment, and by histological quantitative scores. Given the limitations in sourcing cartilage tissue and the risk of donor site morbidity, BM emerges as a preferential source of MSC for novel cartilage resurfacing therapies of osteochondral defects using copolymeric poly-D,L-lactide-co-glycolide scaffolds.

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Section: Discussionsupporting

confidence: 85%

Cartilage resurfacing potential of PLGA scaffolds loaded with autologous cells from cartilage, fat, and bone marrow in an ovine model of osteochondral focal defect

et al. 2015

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“…This construct can either be delivered to and retained within the damaged site or CONTACT Mohsen Sheykhhasan m.sheykhhasan@acecr.ac.ir pre-cultured to generate the needed tissue which is later transplanted to the needed location. Thus, the choice of suitable cells and scaffolds is the major challenge in TE (Schnabel et al 2002;Tuan 2004;Tögel & Westenfelder 2007;Stocum & Zupanc 2008). The appropriate cells intended for cell therapy must have certain characteristics: they should be relatively easily available, safe, and demonstrate efficiency in the stimulation of reparation of different tissue.…”

Section: Introductionmentioning

confidence: 99%

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Ghiasi

Kalhor

Qomi

et al. 2015

Frontiers in Life Science

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This study presents a comparative assessment of adipose-derived stem cell (ADSCs) proliferation rates and their viability on five different scaffolds. Five different biomaterial scaffolds were prepared: alginate, poly lactic-co-glycolic acid, fibrin glue, inactive platelet-rich plasma, and active plateletrich plasma (APRP). Stem cells were isolated from human adipose tissue. Flow cytometry analysis was performed. Specifically, adipogenesis/osteogenesis/chondrogenesis-associated genes expression was analyzed by real-time polymerase chain reaction. These cells were seeded in the prepared scaffolds. After 14 days, the proliferation and viability of MSCs were evaluated using an MTT assay. Also, stemness genes expression was analyzed with the reverse transcriptasepolymerase chain reaction (RT-PCR) method. In addition, the DNA content assay was also performed. The obtained results showed a significant difference between cell proliferation and viability of different scaffolds. APRP and alginate were shown to be the most and least suitable scaffolds in terms of enhancing cell proliferation and maintaining cell viability respectively (p < .05). RT-PCR reactions demonstrated the expression of the various stemness-related markers (Nanog, Octamer4A, and Sox2) when ADSC cells were grown separately on the five different scaffolds. Our study indicates that compared with the scaffolds, APRP could be the best scaffold for support of ADSC proliferation. ARTICLE HISTORY

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“…Phenotypic changed are accompanied with switches in gene expression, including loss of collagens type II, IX and XI and aggrecan and concomitant upregulation of collagen type I, III and V [96,97].…”

mentioning

confidence: 99%

Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

210

156

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Pain in the joint is often due to cartilage degeneration and represents a serious medical problem affecting people of all ages. Although many, mostly surgical techniques, are currently employed to treat cartilage lesions, none has given satisfactory results in the long term. Recent advances in biology and material science have brought tissue engineering to the forefront of new cartilage repair techniques. The combination of autologous cells, specifically designed scaffolds, bioreactors, mechanical stimulations and growth factors together with the knowledge that underlies the principles of cell biology offers promising avenues for cartilage tissue regeneration. The present review explores basic biology mechanisms for cartilage reconstruction and summarizes the advances in the tissue engineering approaches. Furthermore, the limits of the new methods and their potential application in the osteoarthritic conditions are discussed.

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Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture

Abstract: Our data support the hypothesis that HACs dedifferentiate when grown in monolayer cultures. Moreover, the expression patterns also show that proliferation and differentiation are exclusive features of human chondrocytes.

Cited by 521 publications

References 24 publications

Cartilage resurfacing potential of PLGA scaffolds loaded with autologous cells from cartilage, fat, and bone marrow in an ovine model of osteochondral focal defect

Cartilage resurfacing potential of PLGA scaffolds loaded with autologous cells from cartilage, fat, and bone marrow in an ovine model of osteochondral focal defect

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Cartilage tissue engineering for degenerative joint disease☆

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