Deep-amplicon sequencing (DAS) analysis to determine the presence of pathogenic Helicobacter species in wastewater reused for irrigation

Hortelano, Irene; Moreno, Yolanda; Moreno-Mesonero, Laura; Ferrús, María Antonia

doi:10.1016/j.envpol.2020.114768

Cited by 13 publications

(6 citation statements)

References 75 publications

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“…They unveiled the presence of bacteria belonging to the Helicobacter genus. More specifically, the molecular techniques used in this study identified the presence of H. pylori , H. suis , H. pullorum and H. hepaticus DNA in wastewater samples, even after disinfectant treatment 40 …”

Section: Omics Of Non‐helicobacter Pylori Helicobacter Speciesmentioning

confidence: 88%

Review: Other Helicobacter species

Smet

Ménard

2020

Helicobacter

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This review covers the most important, accessible, and relevant literature published between April 2019 and April 2020 in the field of non‐Helicobacter pylori Helicobacter species (NHPH). The initial part of the review covers new insights regarding the presence of gastric and enterohepatic NHPH in humans and animals, while the subsequent section focuses on the progress in our understanding of animal models, the pathogenicity and omics of these species. Over the last year, the clinical relevance of gastric NHPH infections in humans was highlighted. With regard to NHPH in animals, the ancestral source of Helicobacter suis was further established showing that Cynomolgus macaques are the common ancestor of the pig‐associated H. suis population, and 3 novel Helicobacter species isolated from the gastric mucosa of red foxes were described. "Helicobacter burdigaliensis" sp nov. and "Helicobacter labetoulli" sp nov. were proposed as novel enterohepatic Helicobacter species associated with human digestive diseases. An analysis of Helicobacter cinaedi recurrent infections in humans proposed long‐term antibiotic therapies. Several studies using rodent models further elucidated the mechanisms underlying the development of NHPH‐related disease, as well as intestinal immunity in inflammatory bowel disease models. Omics approaches supported Helicobacteraceae taxonomy and unraveled the transcriptomic signatures of H. suis and Helicobacter heilmannii upon adherence to the human gastric epithelium. With regard to virulence, data showed that the nuclear remodeling promoted by cytolethal distending toxin of Helicobacters involves the MAFB oncoprotein and is associated with nucleoplasmic reticulum formation in surviving cells.

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Section: Omics Of Non‐helicobacter Pylori Helicobacter Speciesmentioning

confidence: 88%

Review: Other Helicobacter species

Smet

Ménard

2020

Helicobacter

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“…For example, a Spanish paper reported that H. pylori was detected using dd-PCR in the water supply, where contamination was not expected in a nonhot zone. Two other Spanish groups detected H. pylori in wastewater using deep amplicon sequencing, 53 and in drinking water using propidium monoazide-PCR (PMA-PCR) and direct viable count-FISH (DVC-FISH). 54 Other potential sites of contamination confirmed by PCR or NGS include the endoscope, 55 surgical forceps, 56 and water bottle.…”

Section: Epidemiological Screening Of Transmission Sourcesmentioning

confidence: 99%

“…Two other Spanish groups detected H . pylori in wastewater using deep amplicon sequencing, 53 and in drinking water using propidium monoazide‐PCR (PMA‐PCR) and direct viable count‐FISH (DVC‐FISH) 54 . Other potential sites of contamination confirmed by PCR or NGS include the endoscope, 55 surgical forceps, 56 and water bottle.…”

Section: Introductionmentioning

confidence: 99%

Application of molecular techniques inHelicobacter pyloridetection: limitations and improvements

Gong

El‐Omar

2021

Helicobacter

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Application of molecular techniques in Helicobacter pylori detection: limitations and improvements 1 | INTRODUC TI ONHelicobacter pylori (H. pylori), a microaerophilic gram-negative proteobacterium, is the most common human pathogen colonizing the gastric mucosa of over half the world's population. 1,2 Although 70% of infected people are symptomless, H. pylori is recognized as a strong causative factor in many gastrointestinal (GI) diseases such as peptic ulcer, atrophic gastritis, gastric cancer, and mucosa-associated lymphoid tissue (MALT) lymphoma. 3 The transmission of H. pylori is usually through person-to-person contact or from environmental sources via oral-oral, fecal-oral, and gastro-oral routes. 4 Therefore, its infection rate is linked to familial socioeconomic status, overcrowding, poor hygiene, and environmental contamination in food and water supply. 5 While maintaining a spiral rod-shaped form in a favorable environment, H. pylori may transform into coccoid shape, a viable but non-culturable (VBNC) form with low metabolic activity, to survive under stressful conditions including oxygen deprivation, antibiotic exposure, and epithelial attachment. 6 Treatment of the infection is usually a combination of acid inhibitory agents (eg, Proton Pump Inhibitors PPI) and antibiotics (ie, amoxicillin, clarithromycin, metronidazole, and tetracycline), the choice of which depends on antibiotic sensitivity. 7 Efficient diagnosis is required for successful clinical management to relieve symptoms and to eradicate bacteria. Clinicians have developed numerous H. pylori detection methods, which are divided into two groups based on whether samples are collected by invasive endoscopy. 8 Serology, urea breath test (UBT), and stool antigen test (SAT) are the non-invasive tests carried out on stool, breath, saliva, or serum samples. 9 The invasive tests including endoscopy, histology, culture, and rapid urease test (RUT) are carried out on gastric biopsies. 10 All these conventional tests are based on the presence of different components such as enzymes (UBT, RUT), antigens (SAT), bacteria (culture), and antibodies (serology, histology). 11 Several reviews have discussed their advantages and disadvantages in accuracy, sensitivity, specificity, simplicity, and cost. [12][13][14][15] To improve conventional methods, especially sensitivity, some molecular techniques based on DNA/RNA have been recently used in both invasive and non-invasive H. pylori diagnosis. 16 These new methods include polymerase chain reaction (PCR), 17 real-time PCR, 18 droplet digital PCR (dd-PCR), 19 fluorescent in situ hybridization (FISH), 20 and next-generation sequencing (NGS) including 16S rRNA amplicon sequencing, 16 transcriptomics, 21 and metagenomics. 22 In this issue, Gantuta et al. 23 discussed the advantages of 16S rRNA sequencing in H. pylori diagnosis, which brings to attention the need to review molecular detection tests, especially their shortcomings. This editorial summarizes our current understanding of molecular methods, focusing on ...

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“…H. pylori NCTC 11637 strain (National Collection of Type Cultures, UK) was cultured in Blood Agar Medium as previously described (Hortelano et al, 2020), The cultures were incubated under micro-aerobic conditions (5% oxygen, 10% carbon dioxide, and 85% nitrogen) and 90-95% humidity, by using CampyGen TM 3-5L Atmosphere Generation Systems (Oxoid, UK) in anaerobic jars (Oxoid, UK) at 37 °C for 48 h-14 days.…”

Section: Bacteria Strain and Growth Conditionsmentioning

confidence: 99%