Deep focus-extended darkfield imaging for in situ observation of marine plankton

Chen, Tao; Li, Jianping; Ma, Wenqi; Guo, Guannan; Yang, Zhenyu; Li, Zhenping; Qiao, Jianping

doi:10.3389/fmars.2023.1074428

Cited by 2 publications

(2 citation statements)

References 53 publications

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“…Consequently, this field holds tremendous significance and enjoys widespread popularity within the domain of marine scientific research. [1][2][3] Predominant in-vivo research and observation techniques for plankton concentrate primarily on imaging monitoring, encompassing methodologies such as biological net capture, holographic imaging, and microscopic imaging. Samples collected by biological net capture need to be sent back to the laboratory for further research, which posing challenges for in-situ observation.…”

Section: Introductionmentioning

confidence: 99%

Underwater in-situ plankton imaging system based on microscopic optical tweezers

Zhu,

Kan,

et al. 2024

Sixth Conference on Frontiers in Optical Imaging and Technology: Novel Detector Technologies

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Marine plankton play an irreplaceable role in the marine ecological environment, and it is also an important and popular research field in biology and oceanography. Microscopic imaging system, with the outstanding advantages of high resolution and high magnification, is one of the most direct and effective in situ observation and research tools for marine plankton. To carry out the detection and research of marine plankton more efficiently and reduce the impact of its free movement on in-situ observation, this paper proposes to combine the optical tweezers with the microscopic imaging technology to design a microscopic optical tweezers based underwater in-situ observation system for plankton. The system uses holographic optical tweezers to enable the capturing of plankton in the water body, which allows it positioned stably and imaged clearly within the field of view. The work described in this paper built a holographic microscopic optical tweezers system in the laboratory and wrote an algorithm for generating phase maps based on diffraction-computed holography, thus break through the limitation that the traditional optical tweezers system can only capture the target at the center of the optical path and realizing free manipulation of targeting plankton in the field of view. Meanwhile, this paper also miniaturizes the laboratory system and completes the design including the underwater chamber and related accessories so that the system can work well underwater. The experiments show that the system can stabilize and image plankton samples well in both static water and flow field, which can meet the requirements of underwater in-situ experiments, indicating that the method has great practical value and application prospects in solving the problem of high-quality in-situ microscopic observation.

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Section: Introductionmentioning

confidence: 99%

Underwater in-situ plankton imaging system based on microscopic optical tweezers

Zhu,

Kan,

et al. 2024

Sixth Conference on Frontiers in Optical Imaging and Technology: Novel Detector Technologies

View full text Add to dashboard Cite

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“…and time scale (day, month, quarter, year etc.) [19]. As a mobile observation platform, the ocean research ship conducts fixed point and navigational observations to investigate the limited space-time coverage.…”

Section: Introductionmentioning

confidence: 99%

Design of a multiparameter data acquisition and control system for in situ seabed observation base stations

Zhong,

Lu,

Kang

et al. 2023

IET Science Measure & Tech

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With the exploration, development, and research of deep‐sea resources, there is an urgent need for long‐term and continuous observation data of the deep‐sea seabed boundary layer. The traditional method of deep‐sea seabed survey and sampling based on scientific research vessels has the discontinuity of observation data in space and time scales. There are some problems in the seabed in situ observation method based on the seabed observation network for low mobility and high operation and maintenance costs, restricting the in‐depth understanding of the dynamic change process of the deep‐sea floor. To solve the above problems, an open and modular data acquisition control system was designed based on an embedded system and signal processing technology. In terms of the physical, chemical, geological, and ecosystem characteristics of the seafloor or near the seafloor boundary layer, various functional sensors and instrumentation were matched to form an independent underwater integrated measurement or experimental device, eventually realizing in situ multiparameter and long‐time series observations of the seafloor. The system data acquisition and control test were completed through laboratory experiments, which verified the feasibility of the system design. The research showed important theoretical and technical reference significance for the exploration and development of resources in the submarine boundary layer and the promotion of deep‐sea scientific research.

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Deep focus-extended darkfield imaging for in situ observation of marine plankton

Cited by 2 publications

References 53 publications

Underwater in-situ plankton imaging system based on microscopic optical tweezers

Underwater in-situ plankton imaging system based on microscopic optical tweezers

Design of a multiparameter data acquisition and control system for in situ seabed observation base stations

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