This study delves into the southern Aegean regionwhere the subduction of the oceanic Mediterranean lithosphere under the Aegean continental one takes place. This region is considered one of the most active ones in the eastern Mediterranean Sea due to intense tectonic movements in the Late Quaternary. More than 1200 manually revised events from 2018 to 2023 have been used in order to obtain the 3D structure of body-wave velocity and VP/VS ratioto 80 km depth through earthquaketomography. A series of resolution tests have been performed and demonstrated fair resolution of the derived velocity structures in the area of interest. The derived anomalies of body-waves (dVP, dVS) and VP/VS ratio provided important information about the southern Aegean regional tectonics and secondarily active faults of smaller scale (>20 km). The region is marked by significant low-velocity anomalies in the crust and uppermost mantle, beneath the active arc volcanoes. The seismicity related to the Hellenic Subduction Zone (HSZ) is connected to a low-angle positive anomaly of VP and VS, correlated withthe observed intermediate-depth seismicity (H ≥ 40 km) in this part of the study area. This result could be related to the diving HSZ slab.