Deepening the knowledge on the removal of Cr(VI) by L. minuta Kunth: removal efficiency and mechanisms, lipid signaling pathways, antioxidant response, and toxic effects

Fernández, María Inés Sobrón; Paisio, Cintia Elizabeth; González, Pablo J.; Perotti, Romina; Meringer, Verónica; Villasuso, Ana Laura; Agostini, Elizabeth

doi:10.1007/s11356-020-07884-4

Cited by 4 publications

(6 citation statements)

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“…The expanded lm5633 genes were predominantly a result of TD and PD events, as compared to sp9509, leading to expansion of genes involved in pathogen defense, response to stress, and nutrient acquisition ( Figure 3D ; Supplemental Table S4 ). For instance, there is an 11 gene TD of a boric acid channel ( Figure 3C ), which may reflect the ability of lm5633 to extract large amounts of boron from its environment, improving survivability, and invasiveness ( Fernández et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…The small organism size (∼1 mm), fast growth rate (∼24 h), small genome size (∼360 Mb), and ability to exploit diverse environments led us to select L. minuta as a way to understand cell trajectories and function across an entire plant. Lemna minuta is emerging as a phytoremediation species due to its superior ability to remove various excess elements and toxins from wastewater ( Ceschin et al, 2020 ; Fernández et al, 2020 ). This led us to hypothesize that the invasiveness of L. minuta may result from a specialized set of genes or cellular function, as it is apparently not solely due to its rapid growth rate.…”

Section: Introductionmentioning

confidence: 99%

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The genome and preliminary single-nuclei transcriptome ofLemna minutareveals mechanisms of invasiveness

et al. 2021

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The ability to trace every cell in some model organisms has led to the fundamental understanding of development and cellular function. However, in plants the complexity of cell number, organ size and developmental time makes this a challenge even in the diminutive model plant Arabidopsis (Arabidopsis thaliana). Duckweed, basal non-grass aquatic monocots, provide an opportunity to follow every cell of an entire plant due to their small size, reduced body plan, and fast clonal growth habit. Here we present a chromosome-resolved genome for the highly invasive Lesser Duckweed (Lemna minuta) and generate a preliminary cell atlas leveraging low cell coverage single-nuclei sequencing. We resolved the 360 megabase genome into 21 chromosomes, revealing a core non-redundant gene set with only the ancient tau whole genome duplication shared with all monocots, and paralog expansion as a result of tandem duplications related to phytoremediation. Leveraging SMARTseq2 single-nuclei sequencing, which provided higher gene coverage yet lower cell count, we profiled 269 nuclei covering 36.9% (8,457) of the L. minuta transcriptome. Since molecular validation was not possible in this non-model plant, we leveraged gene orthology with model organism single cell expression datasets, gene ontology, and cell trajectory analysis to define putative cell types. We found that the tissue that we computationally defined as mesophyll expressed high levels of elemental transport genes consistent with this tissue playing a role in L. minuta wastewater detoxification. The L. minuta genome and preliminary cell map provide a paradigm to decipher developmental genes and pathways for an entire plant.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The genome and preliminary single-nuclei transcriptome ofLemna minutareveals mechanisms of invasiveness

et al. 2021

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“…For instance, there is an 11 gene TD of a boric acid channel (Fig. 3C), which may reflect the ability of lm5633 to extract large amounts of boron from its environment, improving survivability, and invasiveness (Fernández et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Lemna minuta can occupy a variety of ecosystems due to their adaptability in absorption and tolerance of excess micro- and macro-nutrients. The uptake and adaptability of L. minuta has led to its use as a wastewater detoxifying/phytoremediation species (Fernández et al, 2020). Wastewaters are often contaminated with a variety of toxic compounds yet the exact mechanisms of plants ability to adapt to these compounds, including high levels of essential elements and heavy metals, remains unclear (Frick, 1985; Davis et al, 2002; Del-Campo Marín and Oron, 2007; Gür et al, 2016; Liu et al, 2018; Türker et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…The small organism size (~1mm), fast growth rate (~24 hrs), small genome size (~360 Mb), and ability to exploit diverse environments led us to select L. minuta as a model system to understand cell trajectories and function across an entire plant. L. minuta is emerging as a phytoremediation species due to its superior ability to remove various excess elements and toxins from wastewater (Ceschin et al, 2020; Fernández et al, 2020). This led us to hypothesize that the invasiveness of L. minuta may result from a specialized set of genes or cellular function, as it is apparently not solely due to its rapid growth rate.…”

Section: Introductionmentioning

confidence: 99%

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Single nuclei transcriptome of the Lesser Duckweed Lemna minuta reveals cell trajectories for an entire plant

Novotny

Hartwick

et al. 2021

Preprint

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The ability to trace every cell in some model organisms has led to the fundamental understanding of development and cellular function. However, in plants the complexity of cell number, organ size and developmental times makes this a challenge even in the diminutive model plant Arabidopsis thaliana. Here we develop the Lesser Duckweed Lemna minuta as a model with a reduced body plan, small genome size and clonal growth pattern that enables simultaneous tracing of cells from the entire plant over the complete developmental cycle. We generated a chromosome-resolved genome for the 360 megabase genome and defined the growth trajectory of the entire plant with single nuclei RNA sequencing. The L. minuta gene complement represents a primarily non-redundant set with only the ancient tau whole genome duplication shared with all monocots, and paralog expansion as a result of tandem duplications related to phytoremediation. Thirteen distinct cell types representing meristem, the leaf-stem fusion called a frond, and root-like tissues were defined using gene orthology with single cell expression from model plants, gene ontology categories, and cell trajectory analysis. Dividing meristem cells give rise to two main branches of root-transition and mesophyll cells, which then give rise to terminally differentiated parenchyma, epidermal and root cells. Mesophyll tissues express high levels of elemental transport genes consistent with this tissue playing a role in L. minuta wastewater detoxification. The L. minuta genome and cell map provide a paradigm to decipher developmental genes and pathways for an entire plant.

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A homemade laboratory applied to the phytoremediation of textile dyes by high school students in the context of the COVID-19 pandemic

Vezza

Alderete

Agostini

2021

Journal of Biological Education

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Deepening the knowledge on the removal of Cr(VI) by L. minuta Kunth: removal efficiency and mechanisms, lipid signaling pathways, antioxidant response, and toxic effects

Cited by 4 publications

References 67 publications

The genome and preliminary single-nuclei transcriptome ofLemna minutareveals mechanisms of invasiveness

The genome and preliminary single-nuclei transcriptome ofLemna minutareveals mechanisms of invasiveness

Single nuclei transcriptome of the Lesser Duckweed Lemna minuta reveals cell trajectories for an entire plant

A homemade laboratory applied to the phytoremediation of textile dyes by high school students in the context of the COVID-19 pandemic

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