Degradation of chitosan-based materials after different sterilization treatments

Juan, Ao; Montembault, Alexandra; Gillet, Dominique; Say, J P; Rouif, Sophie; Bouet, T.; Royaud, Isabelle; David, Laurent

doi:10.1088/1757-899x/31/1/012007

Cited by 34 publications

(25 citation statements)

References 10 publications

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“…These are presently being considered and will be reported on in a future article. Our results are currently limited to the confirmation that, under the constant sample dimensions considered here, Ch and CEC are biocompatible and nontoxic …”

Section: Discussionmentioning

confidence: 88%

The effect of ethylene oxide sterilization on the surface chemistry and in vitro cytotoxicity of several kinds of chitosan

et al. 2013

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The surfaces of three chitosan samples, differing only in their degrees of deacetylation and of carboxyethyl chitosan were chemically characterized by X-ray photoelectron spectroscopy, time-of-flight secondary ion mass spectroscopy, X-ray diffraction, and Fourier transform infrared, both before and after sterilization with ethylene oxide. Unexpected elemental ratios suggest that surface chemical modification occurred during the processing of the original chitin, with further surface modification on subsequent sterilization, despite previous reports to the contrary. Cell viability was evaluated by direct contact methyl thiazole tetrazolium and lactate dehydrogenase assays between the chitosan particles and A549 human epithelial cells, which demonstrated that the modifications incurred on sterilization are reflected in biocompatibility changes. All the samples were found to be biocompatible and nontoxic before sterilization and remained so subsequently.

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Section: Discussionmentioning

confidence: 88%

The effect of ethylene oxide sterilization on the surface chemistry and in vitro cytotoxicity of several kinds of chitosan

et al. 2013

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“…The working system was a standard three-electrode cell, the SS sample as the working electrode, the saturated calomel electrode (SCE) as the reference electrode, and the platinum foil as the counter electrode, respectively. The distance between the working electrode (1 cm 2 SS sample) and the reference electrode (Ag/AgCl) was 30 mm, between the work electrode (1 cm 2 SS sample) and the counter electrode (Pt wire) was 20 mm [43]. The electrochemical behaviors of SS-CS sample were measured by cyclic voltammetry from +0.6 to +1.1 V at a scanning rate of 2 mV s −1 and 40 cycles, and 0.1 M NaOH aqueous solution was set as the supporting electrolyte.…”

Section: Cyclic Voltammetrymentioning

confidence: 99%

Preparation and characteristics of the sulfonated chitosan derivatives electrodeposited onto 316l stainless steel surface

Huang

Peng

Chen

et al. 2017

Journal of Biomaterials Science, Polymer Edition

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In order to ameliorate the properties of corrosion resistance and achieve applications in anti-biofouling of 316L stainless steel (SS), a sulfated derivative of chitosan was deposited onto stainless steel surface by an electrochemical method. In detail, chitosan-catechol (CS-CT) was synthesised in the hydrochloric acid solution by the Mannich reaction and then electrodeposited on the surface of the polished 316L stainless steel. The chitosan-catechol deposited SS sample was further modified with maleic anhydride and sulfite. The grafting progress was monitored by FTIR, UV spectrophotometer and X-ray photoelectron spectroscopy. Hydrophilicity and corrosion resistance of modified SS were characterized by water contact angle measurements, Tafel curves and electrochemical impedance spectroscopy. The morphology of the SS surface before and after the modification was investigated by atomic force microscopy and scanning electron microscope. Further, the anti-biofouling performance in terms of the anti-adsorption protein and anti-bacteria effects of all modified SS samples were estimated, and the modified 316L exhibits the capability of lower protein adsorption and improved antibacterial effect.

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“…Nevertheless, first macroscopic signs of degradation could be reported at 56 weeks postimplantation while none of the nerve guides were considerably degraded at the end of the study at 76 weeks. Interestingly, both groups presented a similar progression of degradation over time, despite the potential of chain scissions due to β -sterilization [ 27 ]. Depending on the dose of irradiation, a faster degradation has been found in experiments with collagen.…”

Section: Discussionmentioning

confidence: 99%

“…However, irradiation can also lead to material crosslinking hampering the degradation process [ 14 ]. EO sterilization, on the other side, does not lead to polymer degradation or crosslinking reactions, and the chemical structure of chitosan is essentially preserved [ 15 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Long-Term In Vivo Evaluation of Chitosan Nerve Guide Properties with respect to Two Different Sterilization Methods

Stößel

Metzen

Wildhagen

et al. 2018

BioMed Research International

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Severe peripheral nerve injuries are reconstructed either with autologous nerve grafts (gold standard) or alternatively with clinically approved artificial nerve guides. The most common method used to sterilize these medical products is ethylene oxide gassing (EO). However, this method has several disadvantages. An alternative, which has been barely studied so far, represents beta irradiation (β). In previous studies, we developed an artificial nerve guide made of chitosan (chitosan nerve guide, CNG), a biomaterial that is known to potentially retain toxic residues upon EO sterilization. Therefore, we analyzed the long-term regeneration-supporting and mechanical properties of CNGs upon their sterilization with EO or β and their following application in unilateral repair of 12 mm gaps of the rat sciatic nerve. Over a period of 76 weeks, we serially evaluated the recovery of motor functions, the possible emergence of an inflammation in the surrounding connective tissue, the regrowth of axons into the distal nerve, and possible changes in the material properties. Our first long-term evaluation did not reveal significant differences between both sterilization methods. Thus, β is as appropriate as commonly used EO for sterilization of CNGs; however, it may slightly increase the stiffness of the biomaterial over time.

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Degradation of chitosan-based materials after different sterilization treatments

Cited by 34 publications

References 10 publications

The effect of ethylene oxide sterilization on the surface chemistry and in vitro cytotoxicity of several kinds of chitosan

The effect of ethylene oxide sterilization on the surface chemistry and in vitro cytotoxicity of several kinds of chitosan

Preparation and characteristics of the sulfonated chitosan derivatives electrodeposited onto 316l stainless steel surface

Long-Term In Vivo Evaluation of Chitosan Nerve Guide Properties with respect to Two Different Sterilization Methods

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