Degradation on polyacrylamides. Part I. Linear polyacrylamide

Microfabricated Polyacrylamide Devices for the Controlled Culture of Growing Cells and Developing Organisms

“…Transferring the polyacrylamide layer to fresh purified water at least two times for approximately 1 h proved sufficient to ensure the absence of observed growth defect in the organisms studied in this article. The membrane can be stored for several weeks in an aqueous solution without detectable degradation, consistent with quantitative studies of long term polyacrylamide degradation [50]. Before using the polyacrylamide membrane for a cell or organism culture experiment, we soaked it two times in the appropriate medium.…”

Biodegradation of polyacrylamide by bacteria isolated from activated sludge and oil-contaminated soil

“…The medium was supplemented to contain 100, 250, 500 and 1000 mg L −1 of glucose, respectively. Glucose was included in this study as standard carbon source, which is known to be readily utilized by most soil bacteria [13]. An initial bacterial inoculum with a cell dry weight of 3 × 10 2 to 5 × 10 2 mg L −1 was applied in each flask.…”

“…Many reports indicate that commercial PAM degrades to acrylamide when it is heated or exposed to ultraviolet irradiation due to the scission of PAM chains instead of unzipping [13]. This lack of reliable information has repeatedly subjected PAM users to difficulties with developing and managing occupational health and safety programs.…”

Biodegradation of partially hydrolyzed polyacrylamide by bacteria isolated from production water after polymer flooding in an oil field

“…The sample was added dropwise into a large excess of methanol (∼10) and the remnant polymer in the samples were precipitated [15]. The precipitated polymers were filtered off and washed with methanol (3 times) and then the precipitated samples (and also HPAM solid) were dried in vacuum drying chamber at 50 • C. After preparation, these samples were analyzed by JSM-6700F SEM (Japanese Electronic Co.) and AVATAR 360 FT-IR Spectrometer (Thermo Nicolet, USA).…”