DELAYED FLUORESCENCE FROM CHLORELLA: II. EFFECTS OF ELECTRON TRANSPORT INHIBITORS DCMU AND NH₂OH

Abstract

Abstract. –Steady state and transient effects of two electron transport inhibitors (NH2OH˙HCl and DCMU) and one uncoupler (FCCP) upon the emission of delayed fluorescence have been studied in whole cells of Chlorella pyrenoidosa. Observations were made on changes in the delayed fluorescence emitted 0.5 ms after a brief flash and 60 ms after the extinction of steady illumination and on changes in the induction effects caused by preillumination. Patterns in the time course of events after inhibitor addition occur which have different characteristics for each of the additives. The observations are consistent with a model of delayed fluorescence production based on the recombination of charges.

Excellent wavelength-dependence of laser-induced delayed light-emission from mesophyll and bundle-sheath cells of maize

“…The delayed light-emission from both the enzymically prepared mesophyll cells and the broken chloroplasts prepared from the homogenate (Figs. I a and 1 b) is similar to what we have observed in the past with whole algal cells and spinach chloroplasts (Ruby. 1976;McCauley & Ruby.…”

Delayed fluorescence in photosynthesis

“…The changes in DF intensity during the induction period are correlated with different processes that include the build-up of the electrical and proton transmembrane gradient (Wraight and Crofts 1971), and depend on the availability of electron acceptors (Ruby 1976) and donors (Mar et al 1975), and on the state of the oxygen-evolving complex (Zankel 1971;Van Gorkom and Donze 1973).…”