Delayed hypersensitivity pleurisy in guinea‐pigs; demonstration of reduced migration ability of macrophages <i>In vitro</i>

In human tuberculosis, adherent mononuclear cells (AMC) selectively depress in vitro responses to the mycobacterial antigen tuberculin purified protein derivative (PPD). The phenotype of this antigen-specific adherent suppressor cell was characterized by examining the functional activity of adherent cells after selective depletion of sheep erythrocyte-rosetting T cells or OKM1-reactive monocytes. Adherent cell suppression was studied in the [3H]thymidine-incorporation microculture assay by using T cells rigorously depleted of T cells with surface receptors for the Fc portion of IgG (T gamma cells) as antigen-responsive cells. PPD-induced [3H]thymidine incorporation by these non gamma T cells was uniformly reduced (mean, 42% +/- 10% [SD]) when autologous AMC were added to non gamma T cells at a ratio of 1:2. Antigen-specific suppression by AMC was not altered by depletion of sheep erythrocyte-rosetting T cells or treatment with indomethacin. However, AMC treated with OKM1 and complement or gamma irradiation (1,500 rads) no longer suppressed tuberculin responses in vitro. These studies identify the antigen-specific adherent suppressor cell in tuberculosis as an OKM1-reactive, non-erythrocyte-rosetting monocyte. The radiosensitivity of this monocyte immunoregulatory function may facilitate its further definition.

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Immunoregulatory Adherent Cells in Human Tuberculosis: Radiation-Sensitive Antigen-Specific Suppression by Monocytes

Kleinhenz

Ellner

1985

Journal of Infectious Diseases

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Delayed hypersensitivity pleurisy in guinea‐pigs; demonstration of reduced migration ability of macrophages In vitro

Cited by 1 publication

References 13 publications

Immunoregulatory Adherent Cells in Human Tuberculosis: Radiation-Sensitive Antigen-Specific Suppression by Monocytes

Immunoregulatory Adherent Cells in Human Tuberculosis: Radiation-Sensitive Antigen-Specific Suppression by Monocytes

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