Delayed platelet engraftment in group O patients after autologous progenitor cell transplantation

Abstract

A group O phenotype may be associated with delayed PLT engraftment at lower CD34 doses.

A prospective randomized trial of two popular mononuclear cell collection sets for autologous peripheral blood stem cell collection in multiple myeloma

“… ¶ Sustained PLT count of more than 20 × 10 9 /L or more than 50 × 10 9 /L in the absence of PLT transfusion for 72 hours 47 …”

“…For chemotherapy, patients were treated with either cyclophosphamide (2‐4 g/m 2 ) or DT‐PACE (10 mg/m 2 cisplatin, 10 mg/m 2 doxorubicin, 400 mg/m 2 cyclophosphamide, 40 mg/m 2 etoposide, 40 mg oral dexamethasone, 200 mg thalidomide) 14,45,46 . Leukapheresis was initiated between 11 and 14 days after chemotherapy when the peripheral WBC count exceeded 5.0 × 10 9 /L 34,47 . Patients collected after chemotherapy, regardless of specific chemotherapy regimen, were classified as “chemotherapy + growth factor” mobilization (chemo + GF; Table 2) for subsequent analysis.…”

“…The final %MNC and MNCs/kg were determined on all products before freezing. CD34 yields and cell viability were determined by flow cytometry as recommended by the International Society of Hematology and Graft Engineering (ISHAGE) 47,49 . Hematopoietic colony‐forming assays were not performed per institutional policy.…”

“…Hematopoietic colony‐forming assays were not performed per institutional policy. Cells were volume‐adjusted and frozen in 10% dimethyl sulfoxide (DMSO) and stored in the vapor phase of liquid nitrogen 47 …”

“…Patient medical records were reviewed for WBC and PLT engraftment. WBC, early PLT, and late PLT engraftment were determined as previously described 47 …”