Delftia litopenaei sp. nov., a poly-β-hydroxybutyrate-accumulating bacterium isolated from a freshwater shrimp culture pond

Chen, Wen-Ming; Lin, Yang-Shun; Sheu, Der-Shyan; Sheu, Shih-Yi

doi:10.1099/ijs.0.037507-0

Cited by 40 publications

(15 citation statements)

References 29 publications

(30 reference statements)

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“…Members of the Delftia genus are Gram-negative, aerobic, rod-shaped and motile bacteria comprised of five species: Delftia acidovorans (Wen et al, 1999), D. tsuruhatensis (Shigematsu et al, 2003), Delftia lacustris (Jørgensen et al, 2009), Delftia litopenaei (Chen et al, 2012), and Delftia deserti (Li et al, 2015). The coral-associated bacterial strain D. tsuruhatensis from the Gulf of Mannar was reported for its anti-quorum sensing activity.…”

Section: Introductionmentioning

confidence: 99%

Anti-quorum Sensing and Anti-biofilm Activity of Delftia tsuruhatensis Extract by Attenuating the Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa

Singh

Mishra

Jha

2017

Front. Cell. Infect. Microbiol.

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Multidrug-resistance bacteria commonly use cell-to-cell communication that leads to biofilm formation as one of the mechanisms for developing resistance. Quorum sensing inhibition (QSI) is an effective approach for the prevention of biofilm formation. A Gram-negative bacterium, Delftia tsuruhatensis SJ01, was isolated from the rhizosphere of a species of sedge (Cyperus laevigatus) grown along the coastal-saline area. The isolate SJ01 culture and bacterial crude extract showed QSI activity in the biosensor plate containing the reference strain Chromobacterium violaceum CV026. A decrease in the violacein production of approximately 98% was detected with the reference strain C. violaceum CV026. The bacterial extract (strain SJ01) exhibited anti-quorum sensing activity and inhibited the biofilm formation of clinical isolates wild-type Pseudomonas aeruginosa PAO1 and P. aeruginosa PAH. A non-toxic effect of the bacterial extract (SJ01) was detected on the cell growth of the reference strains as P. aeruginosa viable cells were present within the biofilm. It is hypothesized that the extract (SJ01) may change the topography of the biofilm and thus prevent bacterial adherence on the biofilm surface. The extract also inhibits the motility, virulence factors (pyocyanin and rhamnolipid) and activity (elastase and protease) in P. aeruginosa treated with SJ01 extract. The potential active compound present was identified as 1,2-benzenedicarboxylic acid, diisooctyl ester. Microarray and transcript expression analysis unveiled differential expression of quorum sensing regulatory genes. The key regulatory genes, LasI, LasR, RhlI, and RhlR were down-regulated in the P. aeruginosa analyzed by quantitative RT-PCR. A hypothetical model was generated of the transcriptional regulatory mechanism inferred in P. aeruginosa for quorum sensing, which will provide useful insight to develop preventive strategies against the biofilm formation. The potential active compound identified, 1,2-benzenedicarboxylic acid, diisooctyl ester, has the potential to be used as an anti-pathogenic drug for the treatment of biofilm-forming pathogenic bacteria. For that, a detailed study is needed to investigate the possible applications.

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Section: Introductionmentioning

confidence: 99%

Anti-quorum Sensing and Anti-biofilm Activity of Delftia tsuruhatensis Extract by Attenuating the Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa

Singh

Mishra

Jha

2017

Front. Cell. Infect. Microbiol.

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“…The Delftia species we did not encounter in our study are species that have so far been associated with more restricted habitats. Delftia deserti has been found to inhabit desert environments (Li et al, 2015), D. lacustris in lake water (Jørgensen et al, 2009), D. litopenaei in pond water (Chen et al, 2012), and D. rhizosphaerae in the rhizosphere of Cistus ladanifer, a plant native to the Mediterranean region (Carro et al, 2017). The apparent ubiquity of the genus Delftia hides the reality that individual species appear to show considerable habitat restriction.…”

Section: Discussionmentioning

confidence: 99%

Catalyzing rapid discovery of gold-precipitating bacterial lineages with university students

Riley

Goller

Leggett

et al. 2020

PeerJ

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Intriguing and potentially commercially useful microorganisms are found in our surroundings and new tools allow us to learn about their genetic potential and evolutionary history. Engaging students from different disciplines and courses in the search for microbes requires an exciting project with innovative but straightforward procedures and goals. Here we describe an interdisciplinary program to engage students from different courses in the sampling, identification and analysis of the DNA sequences of a unique yet common microbe, Delftia spp. A campus-wide challenge was created to identify the prevalence of this genus, able to precipitate gold, involving introductory level environmental and life science courses, upper-level advanced laboratory modules taken by undergraduate students (juniors and seniors), graduate students and staff from the campus. The number of participants involved allowed for extensive sampling while undergraduate researchers and students in lab-based courses participated in the sample processing and analyses, helping contextualize and solidify their learning of the molecular biology techniques. The results were shared at each step through publicly accessible websites and workshops. This model allows for the rapid discovery of Delftia presence and prevalence and is adaptable to different campuses and experimental questions.

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“…The Delftia species we did not encounter in our study are species that have so far been associated with more restricted habitats. Delftia deserti has been found to inhabit desert environments (Li et al, 2015), D. lacustris in lake water (Jørgensen, Brant, Nybroe, & Hansen, 2012), D. litopenaei in pond water (Chen, Lin, Sheu, & Sheu, 2012), and D. rhizosphaerae in the rhizosphere of Cistus ladanifer, a plant native to the Mediterranean region (Carro et al, 2017). The apparent ubiquity of the genus Delftia hides the reality that individual species appear to show considerable habitat restriction.…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #1 of "Catalyzing rapid discovery of gold-precipitating bacterial lineages with university students (v0.1)"

Ulrich¹

2020

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Intriguing and potentially commercially useful microorganisms are found in our surroundings, and new tools allow us to learn about their genetic potential and evolutionary history. Engaging students from different disciplines and courses in the search for microbes requires an exciting project with innovative but straightforward procedures and goals. Here we describe an interdisciplinary program to engage students from different courses in the sampling, identification, and analysis of the DNA sequences of a unique yet common microbe, Delftia spp. A campus-wide challenge was created to identify the prevalence of this genus, able to precipitate gold, involving introductory level environmental and life science courses, upper-level advanced laboratory modules taken by undergraduate students (juniors and seniors), graduate students, and staff from the campus. The number of participants involved allowed for extensive sampling while undergraduate researchers and students in lab-based courses participated in the sample processing and analyses, helping contextualize and solidify their learning of the molecular biology techniques. The results were shared at each step through publicly accessible websites and workshops. This model allows for the rapid discovery of Delftia presence and prevalence and is adaptable to different campuses and experimental questions.

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Delftia litopenaei sp. nov., a poly-β-hydroxybutyrate-accumulating bacterium isolated from a freshwater shrimp culture pond

Abstract: A Gram-negative, short-rod-shaped, motile, non-spore-forming and poly-b-hydroxybutyrateaccumulating bacterial strain, designated wsw-7 T , was isolated from a freshwater shrimp culture

Cited by 40 publications

References 29 publications

Anti-quorum Sensing and Anti-biofilm Activity of Delftia tsuruhatensis Extract by Attenuating the Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa

Anti-quorum Sensing and Anti-biofilm Activity of Delftia tsuruhatensis Extract by Attenuating the Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa

Catalyzing rapid discovery of gold-precipitating bacterial lineages with university students

Peer Review #1 of "Catalyzing rapid discovery of gold-precipitating bacterial lineages with university students (v0.1)"

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