Delineating the complex mechanistic interplay between NF-κβ driven mTOR depedent autophagy and monocyte to macrophage differentiation: A functional perspective

Bhattacharya, A.; Ghosh, Parthasarathi; Singh, Arpana; Ghosh, Arnab; Bhowmick, Arghya; Sinha, Deepak Kumar; Ghosh, Abhrajyoti; Sen, Prosenjit

doi:10.1016/j.cellsig.2021.110150

Cited by 5 publications

(8 citation statements)

References 113 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given that autophagy has been shown to play a decisive role in the differentiation of monocytes into macrophages [ 45 , 46 ], verification of autophagy was measured in the form of U937 monocyte activation and subsequent differentiation into macrophages, demonstrated by specific staining of cluster of differentiation 14 (CD14), a glycolipid-anchored membrane glycoprotein expressed on both monocytes and macrophages, enabling the identification of these cells [ 47 ]. In Figure 6 A, fast-red coupled CD14 staining was evident in response to the treatments.…”

Section: Resultsmentioning

confidence: 99%

“…Prior to macrophage transformation, monocyte differentiation necessitates the stimulation stage, coinciding with the initiation of autophagosome development, as well the adherence stage, coinciding with degradation of cargo in the autophagolysosomes [ 46 ]. In the 3D model, mimicking the intestinal mucosa [ 44 ], the migration of CD14 differentiated monocytes/macrophages in the ECM towards the Caco-2 cells were demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…In the 3D model, mimicking the intestinal mucosa [ 44 ], the migration of CD14 differentiated monocytes/macrophages in the ECM towards the Caco-2 cells were demonstrated. In such an instance, monocytes and their macrophage progeny (critical for innate immunity) would potentially then serve in immune-related functions, such as phagocytosis of invading pathogens and in the homeostasis and repair of damaged tissue [ 46 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Wheat Germ Spermidine and Clove Eugenol in Combination Stimulate Autophagy In Vitro Showing Potential in Supporting the Immune System against Viral Infections

et al. 2022

View full text Add to dashboard Cite

Impaired autophagy, responsible for increased inflammation, constitutes a risk factor for the more severe COVID-19 outcomes. Spermidine (SPD) is a known autophagy modulator and supplementation for COVID-19 risk groups (including the elderly) is recommended. However, information on the modulatory effects of eugenol (EUG) is scarce. Therefore, the effects of SPD and EUG, both singularly and in combination, on autophagy were investigated using different cell lines (HBEpiC, SHSY5Y, HUVEC, Caco-2, L929 and U937). SPD (0.3 mM), EUG (0.2 mM) and 0.3 mM SPD + 0.2 mM EUG, significantly increased autophagy using the hallmark measure of LC3-II protein accumulation in the cell lines without cytotoxic effects. Using Caco-2 cells as a model, several crucial autophagy proteins were upregulated at all stages of autophagic flux in response to the treatments. This effect was verified by the activation/differentiation and migration of U937 monocytes in a three-dimensional reconstituted intestinal model (Caco-2, L929 and U937 cells). Comparable benefits of SPD, EUG and SPD + EUG in inducing autophagy were shown by the protection of Caco-2 and L929 cells against lipopolysaccharide-induced inflammation. SPD + EUG is an innovative dual therapy capable of stimulating autophagy and reducing inflammation in vitro and could show promise for COVID-19 risk groups.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Wheat Germ Spermidine and Clove Eugenol in Combination Stimulate Autophagy In Vitro Showing Potential in Supporting the Immune System against Viral Infections

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Indeed, erastin-induced ferroptosis can promote the activation of the endoplasmic reticulum (ER) stress response that is regulated by PERK-eIF2a (eukaryotic initiation factor 2a)-ATF4 (activating transcription factor 4) pathway which can inhibit TMZ-induced autophagy (Figure 2G) (60, 61). Besides, it has been reported that the process of protective autophagy can be induced by iron deprivation with antitumor drugs, which can also be reversed by ferric ammonium citrate (FAC) through iron supplementation (62).…”

Section: The Role Of Ferroptosis In Autophagymentioning

confidence: 99%

The crosslinks between ferroptosis and autophagy in asthma

Tang

Qin

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

Autophagy is an evolutionarily conserved cellular process capable of degrading various biological molecules and organelles via the lysosomal pathway. Ferroptosis is a type of oxidative stress-dependent regulated cell death associated with the iron accumulation and lipid peroxidation. The crosslinks between ferroptosis and autophagy have been focused on since the dependence of ferroptosis on autophagy was discovered. Although the research and theories on the relationship between autophagy and ferroptosis remain scattered and fragmented, the crosslinks between these two forms of regulated cell death are closely related to the treatment of various diseases. Thereof, asthma as a chronic inflammatory disease has a tight connection with the occurrence of ferroptosis and autophagy since the crosslinked signal pathways may be the crucial regulators or exactly regulated by cells and secretion in the immune system. In addition, non-immune cells associated with asthma are also closely related to autophagy and ferroptosis. Further studies of cross-linking asthma inflammation with crosslinked signaling pathways may provide us with several key molecules that regulate asthma through specific regulators. The crosslinks between autophagy and ferroptosis provide us with a new perspective to interpret and understand the manifestations of asthma, potential drug discovery targets, and new therapeutic options to effectively intervene in the imbalance caused by abnormal inflammation in asthma. Herein, we introduce the main molecular mechanisms of ferroptosis, autophagy, and asthma, describe the role of crosslinks between ferroptosis and autophagy in asthma based on their common regulatory cells or molecules, and discuss potential drug discovery targets and therapeutic applications in the context of immunomodulatory and symptom alleviation.

show abstract

“…However, the same study found that macrophages could differentiate normally in the absence of autophagy ( 155 ). Another study reported that induction of mTOR-dependent autophagy was crucial for the proper differentiation of monocytes into macrophages ( 156 ).…”

Section: Role Of Autophagy In Immune Cellsmentioning

confidence: 99%

Autophagy: A Versatile Player in the Progression of Colorectal Cancer and Drug Resistance

et al. 2022

View full text Add to dashboard Cite

Colorectal cancer (CRC) is among the topmost malignancies for both genders. Despite the high incidence rate and advances in diagnostic tools, treatment in many cases is still ineffective. Most cancerous lesions in CRC begin as benign, followed by the development of invasive forms and metastases. The development of CRC has been linked to defects in autophagy, which plays both a pro-and anti-tumor role and is mainly context-dependent. Autophagy suppression could enhance apoptosis via p53 activation, or autophagy also promotes tumor progression by maintaining tumor growth and increasing resistance to chemotherapy. Autophagy promotes the invasion and metastasis of CRC cells via increased epithelial-mesenchymal transition (EMT). Moreover, dysbiosis of gut microbiota upregulated autophagy and metastasis markers. Autophagy responses may also modulate the tumor microenvironment (TME) via regulating the differentiation process of several innate immune cells. Treatments that promote tumor cell death by stimulating or inhibiting autophagy could be beneficial if used as an adjunct treatment, but the precise role of various autophagy-modulating drugs in CRC patients is needed to be explored. In this article, we present an overview of the autophagy process and its role in the pathogenesis and therapeutic resistance of CRC. Also, we focused on the current understanding of the role of the EMT and TME, including its relation to gut microbiota and immune cells, in autophagic manipulation of CRC. We believe that there is a potential link between autophagy, TME, EMT, and drug resistance, suggesting that further studies are needed to explore this aspect.

show abstract

Delineating the complex mechanistic interplay between NF-κβ driven mTOR depedent autophagy and monocyte to macrophage differentiation: A functional perspective

Cited by 5 publications

References 113 publications

Wheat Germ Spermidine and Clove Eugenol in Combination Stimulate Autophagy In Vitro Showing Potential in Supporting the Immune System against Viral Infections

Wheat Germ Spermidine and Clove Eugenol in Combination Stimulate Autophagy In Vitro Showing Potential in Supporting the Immune System against Viral Infections

The crosslinks between ferroptosis and autophagy in asthma

Autophagy: A Versatile Player in the Progression of Colorectal Cancer and Drug Resistance

Contact Info

Product

Resources

About