Herein we reported that polyamidoamine dendrimer (PAMAM) modified single walled carbon nanotubes (PSWCNTs) were successfully used for high efficient delivery and intracellular imaging of survivin siRNA vector. No.4 generation of PAMAM dendrimers were used to modify single walled carbon nanotubes, and characterized by high resolution transmission electron microscopy (HR-TEM), and atom force microscopy (AFM), survivin shRNA vectors were constructed and identified, and then dendrimer-modified SWCNTs were mixed with survivin shRNA vectors, resultant dendrimer-SWCNTs-survivin shRNA vector mixtures co-cultured with human gastric cancer MGC803 cells, then the expression of survivin shRNA vectors in MGC803 cells was observed by fluorescent microscopy, cell viability was analyzed by MTT method. Results showed that survivin shRNA vector was successfully expressed in MGC803 cells, and MGC 803 cell growth was markedly inhibited. In conclusion, dendrimer (PAMAM) modified single walled carbon nanotubes can be used for high efficient delivery and intracellular imaging of siRNA, and own great potential in applications such as gene or drug delivery and tumor targeted imaging and simultaneous therapy in near future.