Demineralization and Remineralization Evaluation Techniques

Arends, J; Bosch, J.J. ten

doi:10.1177/002203459207100s27

Cited by 292 publications

(241 citation statements)

References 22 publications

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“…The use of SH loss to evaluate enamel-dentin demineralization is supported by the available literature. 8,20 Although our results contradict those found by ten Cate et al, 1 these authors used an in vitro design, which may account for the observed differences compared to our in situ study.…”

Section: Discussioncontrasting

confidence: 98%

Timing of fluoride toothpaste use and enamel-dentin demineralization

et al. 2011

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It is well established that fluoride (F) prevents caries development by inhibiting demineralization and enhancing remineralization processes. However, it is not known which of these protective mechanisms is more important. In this double-blind, crossover in situ study conducted in three phases of 14 days each, 12 volunteers wore palatal appliances containing enamel and root dentin slabs, on which biofilm was allowed to accumulate under exposure to 20% sucrose solution 8×/day. F toothpaste was used once a day, either before the daily demineralizing episodes (in the morning) or after them (at night). Non-F placebo toothpaste was used in the control group. F toothpaste significantly reduced enamel and dentin demineralization compared with the control (p < 0.05). F toothpaste was more effective when used after the demineralization episodes than before, and this difference was statistically significant for dentin (p < 0.05). The results suggest that brushing with F dentifrice at night to remineralize daily mineral losses may be preferable to brushing in the morning to inhibit the demineralizing episodes of the day.

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Section: Discussioncontrasting

confidence: 98%

Timing of fluoride toothpaste use and enamel-dentin demineralization

et al. 2011

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“…It has been reported that the accuracy of TMR for enamel was approximately 5 m for Ld and approximately 200 for Z (Vol m), regardless of the digitalizing method 4) . In light of this finding 4) , the TMR procedure was employed in the present study. To calculate the mineral content profile, TMR films were scanned.…”

Section: Discussionmentioning

confidence: 99%

“…According to a review by Arends and Bosch, TMR is the most widely used technique to evaluate tooth mineralization, despite some inherent disadvantages 4) . It has been reported that the accuracy of TMR for enamel was approximately 5 m for Ld and approximately 200 for Z (Vol m), regardless of the digitalizing method 4) . In light of this finding 4) , the TMR procedure was employed in the present study.…”

Section: Discussionmentioning

confidence: 99%

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Development of New Software as a Convenient Analysis Method for Dental Microradiography

et al. 2007

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To the end of developing a convenient research tool to calculate the mineralization status of teeth in detail, a new program was developed using Visual Basic for Applications combined with Microsoft Excel 2004. To demonstrate the usefulness of this program, it was used to analyze tooth enamel mineralization after acid exposure. Transverse microradiography images (TMR) of specimens were digitalized with a charge-coupled device camera with a microscope (CCD camera) and a digital film scanner (FS). Subsequently, the mineral content profile of each specimen after de-and remineralization studies were calculated using the Angmar's formula. The newly developed program was applied to calculating the mineral loss (ΔZ), lesion depth (Ld), surface zone depth (SZd), and lesion body depth (LBd) of tooth specimens. In addition, the outer surface zone (OSZ), inner lesion body (ILB), and sandwich area (SA) between OSZ and ILB -which together constituted ΔZ -were calculated by the newly developed program. Data obtained with the newly developed program were in good agreement for both CCD camera and FS, indicating that the program was reliable for tooth enamel mineralization research studies.

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“…A number of reviews have been published on the wide array of methods that have been developed to quantify changes in mineral status of tooth specimens . 33,34,36 The UK group identified those methods that have received general acceptance by the research community. These included the following qualitative methods: Of the methods currently validated, TMR was considered to be the most informative, but is also the most demanding.…”

Section: Methods Of Measurementmentioning

confidence: 99%

Modern methods for assessing the cariogenic and erosive potential of foods

Curzon¹,

Hefferren²

2001

Br Dent J

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RESEARCH nutrition and plaque pH, but also determined that enamel demineralisation should be further developed as a model. Dental erosion was not perceived as a problem, at that time, and was not mentioned in the deliberations. The animal model has continued to be used. Researchers, and other authorities, wishing to determine the cariogenic potential of foods, drinks and some oral medicines, have used the plaque pH models. The enamel demineralisation model, often called the intra-oral cariogenicity test (ICT), has been further developed since the San Antonio conference, so that it is now widely used for cariogenicity testing. Variants of the ICT, also known as the in situ model, are now being developed to test foods and drinks for erosion, but need evaluation.Accordingly on 8 and 9 November 1999, a group of dental scientists and clinicians met in London (UK) to review the San Antonio recommendations, reassess them and draw up revised guidelines. The aim was to determine which methods are currently suitable as research tools but also for regulatory assessments.The conference was directed by Professor John Hefferren, previously Chairman of the San Antonio meeting, and who lead the discussions of all delegates. In each of the four workshop sessions, under the guidance of a chairman, a presenter gave a prepared report to update the information on a particular method or group of methodologies. Each presentation was then commented on by a reactor and then by open discussion of all delegates. On the second day individual workshops were held involving small groups of delegates and working documents prepared presenting draft new guidelines.In a final plenary session all of the working documents were reconsidered and refined. Subsequently, the chairman of each working group was asked to prepare a draft document that was then circulated and commented upon by all delegates. After final editing the consensus opinions are presented in this document. Organisation of the textThe following text is organised to consider the four main groups of models for cariogenicity and erosion evaluation. The first is the animal model, usually based on the rat using an automatic feeding machine. The second group concerns the various approaches to assess plaque pH responses to the use of foods. The third model is the enamel demineralisation method and the fourth is that concerning assessment of the erosive potential of foods. Readers should consider this document in association with the San Antonio publication 1 which gives the original guidelines for methodologies in great detail. Herein only suggested changes or comments on the original have been made. Animal models:Workshop group: M. E. J. Curzon, J. J. Hefferren, P. M. Marsh and P. Mtolo The group agreed that animal testing (usually with the laboratory rat) remains an important model to assess cariostatic and cario-

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Demineralization and Remineralization Evaluation Techniques

Cited by 292 publications

References 22 publications

Timing of fluoride toothpaste use and enamel-dentin demineralization

Timing of fluoride toothpaste use and enamel-dentin demineralization

Development of New Software as a Convenient Analysis Method for Dental Microradiography

Modern methods for assessing the cariogenic and erosive potential of foods

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