Dendrimer-Au Nanoparticle Network Covered Alumina Membrane for Ion Rectification and Enhanced Bioanalysis

Abstract

Ion transport in an artificial asymmetric nanoporous membrane, which is similar to biological ion channels, can be used for biosensing. Here, a dendrimer-Au nanoparticle network (DAN) is in situ assembled on a nanoporous anodic aluminum oxide (AAO) surface, forming a DAN/AAO hybrid membrane. Benefiting from the high surface area and anion selectivity of DAN, the prepared DAN/AAO hybrid presents selective ion transport. Under a bias potential, a diode-like current−potential (I−V) response is observed. The obtained ionic current rectification (ICR) property can be tuned by the ion valence and pH value of the electrolyte. The rectified ionic current endows the as-prepared DAN/AAO hybrid with the ability of enhanced bioanalysis. Sensitive capture and detection of circulating tumor cells (CTCs) with a detection limit of 80 cells mL −1 as well as excellent reusability can be achieved.

Towards explicit regulating-ion-transport: nanochannels with only function-elements at outer-surface

“…One of the important features of FE is of versatile physical and chemical properties, nanochannel-system adapt to a broad range of applications spanning from osmotic energy conversion devices 15 – 17 , 33 , 38 to biosensors 26 , 27 , 39 – 41 . Meanwhile, a nanochannel system combining with electrochemistry in a confined space is now a crucial promising field 42 , 43 , which is utilized to dynamically monitor the single molecule 44 , understand the chemical reaction 45 , characterize the single particle 46 , and probe single living cell 47 , etc.…”

Recent advances in development of dendritic polymer‐based nanomedicines for cancer diagnosis

“…Upon the treatment of benzonase, the captured cells would be released without any damage from the reusable nanodevice, and accordingly, the change in the ionic current rectification property could be used to calculate the number of CTCs on the nanodevice. Importantly, this nanodevice could reached a detection limit as low as 80 cells/ml (C. Wang, Zhao, et al, 2020).…”

Bio-inspired nanochannels: fabrication, modification and bioanalytical applications

“…首先, 通过化学偶联的方式将探针单链 DNA (ssDNA)固定在离子通道外表面, 当引入目标mi-图 12 (a) 凝血酶适配体功能化的纳米通道-离子通道器件在不同溶液pH下机理 [106] ; (b) 纳米通道-离子通道杂交结构表面修 饰和micro RNA检测原理 [107] ; (c) 基于GO与偶氮DNA修饰的PAA设计的光响应纳米流体装置 [108] ; (d) CTCs捕获和释放过程的 原理图 [110] (网络版彩图) [109,110] . 通过适配 体-CTCs的识别, 选择性地捕获溶液中的CTCs (图12 (d)) [110] . [111,112] .…”

“…(c) Light-responsive nanofluidic device designed based on GO and azo-DNA modified PAA[108]. (d) Schematic demonstration of the CTCs capture and release process[110] (color online).…”