Dendrimer based theranostic nanostructures for combined chemo- and photothermal therapy of liver cancer cells in vitro

Jędrzak, Artur; Grześkowiak, Bartosz F.; Coy, Emerson; Wojnarowicz, Jacek; Szutkowski, Kosma; Jurga, Stefan; Jesionowski, Teofil; Mrówczyński, Radosław

doi:10.1016/j.colsurfb.2018.10.045

Cited by 90 publications

(43 citation statements)

References 45 publications

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“…The synthesized nanocarriers were reported as nontoxic and it can load the drug photothermal and chemotherapeutic agents effectively to treat liver cancer cells at the solution of low nanoparticles. 28 Sharma et al, (2017) reported that the surface of dendrimers has water soluble functional groups that are responsible for the excellent water solubility. 29 Liver cancer drugs are hydrophobic, the inner core and branches of dendrimers have the characteristic of encapsulating hydrophobic drugs.…”

Section: Dendrimermentioning

confidence: 99%

Nanocarrier-Based Therapeutics and Theranostics Drug Delivery Systems for Next Generation of Liver Cancer Nanodrug Modalities

Ruman

Fakurazi

Masarudin

et al. 2020

IJN

115

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The development of therapeutics and theranostic nanodrug delivery systems have posed a challenging task for the current researchers due to the requirement of having various nanocarriers and active agents for better therapy, imaging, and controlled release of drugs efficiently in one platform. The conventional liver cancer chemotherapy has many negative effects such as multiple drug resistance (MDR), high clearance rate, severe side effects, unwanted drug distribution to the specific site of liver cancer and low concentration of drug that finally reaches liver cancer cells. Therefore, it is necessary to develop novel strategies and novel nanocarriers that will carry the drug molecules specific to the affected cancerous hepatocytes in an adequate amount and duration within the therapeutic window. Therapeutics and theranostic systems have advantages over conventional chemotherapy due to the high efficacy of drug loading or drug encapsulation efficiency, high cellular uptake, high drug release, and minimum side effects. These nanocarriers possess high drug accumulation in the tumor area while minimizing toxic effects on healthy tissues. This review focuses on the current research on nanocarrier-based therapeutics and theranostic drug delivery systems excluding the negative consequences of nanotechnology in the field of drug delivery systems. However, clinical developments of theranostics nanocarriers for liver cancer are considered outside of the scope of this article. This review discusses only the recent developments of nanocarrier-based drug delivery systems for liver cancer therapy and diagnosis. The negative consequences of individual nanocarrier in the drug delivery system will also not be covered in this review.

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Section: Dendrimermentioning

confidence: 99%

Nanocarrier-Based Therapeutics and Theranostics Drug Delivery Systems for Next Generation of Liver Cancer Nanodrug Modalities

Ruman

Fakurazi

Masarudin

et al. 2020

IJN

115

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“…Jędrzak et al (2019) integrated polydopamine (PDA)-coated magnetite nanoparticles and PAMAM dendrimers to synthesize novel and multifunctional nanoplatforms. The designed PAMAM dendrimer G 5.0 functionalized nanocarriers exhibited a high drug-encapsulating capacity for doxorubicin hydrochloride, and were successful applieded in the photo- and chemothermal treatment of the human hepatoma HepG2 cell line (liver cancer cells), even at lower nanoparticle concentrations ( Figure 5 ) [ 300 ].…”

Section: Stimuli-responsive Polymeric Nanocarriers For Theranosismentioning

confidence: 99%

“… The schematic synthesis presentation of theranostic nanoplatform based on the Fe3O4@PDA@G 4.0–6.0 nanoparticles and its application in combined CT-PTT therapy on HepG2 cells. Reproduced from [ 300 ] with permission from Elsevier, 2019. …”

Section: Figurementioning

confidence: 99%

Stimuli-Responsive Polymeric Nanocarriers for Drug Delivery, Imaging, and Theragnosis

et al. 2020

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In the past few decades, polymeric nanocarriers have been recognized as promising tools and have gained attention from researchers for their potential to efficiently deliver bioactive compounds, including drugs, proteins, genes, nucleic acids, etc., in pharmaceutical and biomedical applications. Remarkably, these polymeric nanocarriers could be further modified as stimuli-responsive systems based on the mechanism of triggered release, i.e., response to a specific stimulus, either endogenous (pH, enzymes, temperature, redox values, hypoxia, glucose levels) or exogenous (light, magnetism, ultrasound, electrical pulses) for the effective biodistribution and controlled release of drugs or genes at specific sites. Various nanoparticles (NPs) have been functionalized and used as templates for imaging systems in the form of metallic NPs, dendrimers, polymeric NPs, quantum dots, and liposomes. The use of polymeric nanocarriers for imaging and to deliver active compounds has attracted considerable interest in various cancer therapy fields. So-called smart nanopolymer systems are built to respond to certain stimuli such as temperature, pH, light intensity and wavelength, and electrical, magnetic and ultrasonic fields. Many imaging techniques have been explored including optical imaging, magnetic resonance imaging (MRI), nuclear imaging, ultrasound, photoacoustic imaging (PAI), single photon emission computed tomography (SPECT), and positron emission tomography (PET). This review reports on the most recent developments in imaging methods by analyzing examples of smart nanopolymers that can be imaged using one or more imaging techniques. Unique features, including nontoxicity, water solubility, biocompatibility, and the presence of multiple functional groups, designate polymeric nanocues as attractive nanomedicine candidates. In this context, we summarize various classes of multifunctional, polymeric, nano-sized formulations such as liposomes, micelles, nanogels, and dendrimers.

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“…56,57 The latest publications have proven that dual therapies, ie combined chemo-and photothermal treatment, contribute to lower tumor growth and cancer regression due to the more efficient drug penetration to the adjacent cells in the tumor tissue. 38,58 Therefore, novel magnetic nanoparticles have to fulfil those requirement. In our study, we covered the different sizes of magnetic…”

Section: Dovepressmentioning

confidence: 99%

Magnetite Nanoparticles and Spheres for Chemo- and Photothermal Therapy of Hepatocellular Carcinoma in vitro

Jędrzak

Grześkowiak

Golba

et al. 2020

IJN

Self Cite

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Introduction: We present a multimodal nanoplatforms for the treatment of hepatocellular carcinoma (HCC) in vitro. The nanoplatforms are based on polydopamine (PDA)-coated magnetite nanoparticles (NPs) and spheres (sMAG) with PAMAM dendrimers and functionalized with NHS-PEG-Mal (N-hydroxysuccinimide-polyethylene glycol-maleimide) linker, which allows their functionalization with a folic acid derivative. The nanomaterials bearing a folic acid-targeting moiety show high efficiency in killing cancer cells in the dual chemo-and photothermal therapy (CT-PTT) of the liver cancer cells in comparison to modalities performed separately. Materials and Methods: All materials are characterized in detail with transmission electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential and magnetic measurements. Also, photothermal properties were determined under irradiation of nanoparticles with laser beam of 2 W/cm 2. The nontoxicity of nanoparticles with doxorubicin and without was checked by WST and LIVE/DEAD assay. Those tests were also used to evaluate materials bearing folic acid and anticancer drug in combined chemo-and photothermal therapy of HCC. Further, the generation of reactive oxygen species profile was also evaluated using flow cytometry test. Results: Both NPs and sMAG showed high photothermal properties. Nevertheless, the higher photothermal response was found for magnetic spheres. Materials of concentration above 10 µg/ mL reveal that their activity was comparable to free doxorubicin. It is worth highlighting that a functionalized magnetic sphere with DOXO more strongly affected the HepG2 cells than smaller functionalized nanoparticles with DOXO in the performed chemotherapy. This can be attributed to the larger size of particles and a different method of drug distribution. In the further stage, both materials were assessed in combined chemo-and photothermal therapy (CT-PTT) which revealed that magnetic spheres were also more effective in this modality than smaller nanoparticles. Conclusion: Here, we present two types of nanomaterials (nanoparticles and spheres) based on polydopamine and PAMAM dendrimers g.5.0 functionalized with NHS-PEG-Mal linker terminated with folic acid for in vitro hepatocellular carcinoma treatment. The obtained materials can serve as efficient agents for dual chemo-and photothermal therapy of HCC. We also proved that PDA-coated magnetic spheres were more efficient in therapies based on near-infrared irradiation because determined cell viabilities for those materials are lower than for the same concentrations of nanomaterials based on small magnetic nanoparticles.

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Dendrimer based theranostic nanostructures for combined chemo- and photothermal therapy of liver cancer cells in vitro

Cited by 90 publications

References 45 publications

<p>Nanocarrier-Based Therapeutics and Theranostics Drug Delivery Systems for Next Generation of Liver Cancer Nanodrug Modalities</p>

<p>Nanocarrier-Based Therapeutics and Theranostics Drug Delivery Systems for Next Generation of Liver Cancer Nanodrug Modalities</p>

Stimuli-Responsive Polymeric Nanocarriers for Drug Delivery, Imaging, and Theragnosis

<p>Magnetite Nanoparticles and Spheres for Chemo- and Photothermal Therapy of Hepatocellular Carcinoma in vitro</p>

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