Dendrimer Bioconjugates: Synthesis and Applications

Nazemi, Ali; Gillies, Elizabeth R.

doi:10.1002/9781118775882.ch5

Cited by 2 publications

(1 citation statement)

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“…To enhance the biological activity of poly(glycerol)–protein conjugates, an optimal combination of composition, molecular weight, and polymer architecture was crucial. , Densely grafted polymers have been studied, including the implantation of proteins in molecular bottlebrushes, the use of click chemistry to conjugate globular dendrimers, − and grafting PEG macromonomers via ATRP to mask proteins . Hyperbranched polymers possess unique properties such as weak entanglement, low viscosity, and a globular conformation, making them excellent candidates for grafting from the surface of proteins .…”

Section: Introductionmentioning

confidence: 99%

Tailored Branched Polymer–Protein Bioconjugates for Tunable Sieving Performance

Kapil,

Murata,

Szczepaniak

et al. 2024

ACS Macro Lett.

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Protein−polymer conjugates combine the unique properties of both proteins and synthetic polymers, making them important materials for biomedical applications. In this work, we synthesized and characterized protein-branched polymer bioconjugates that were precisely designed to retain protein functionality while preventing unwanted interactions. Using chymotrypsin as a model protein, we employed a controlled radical branching polymerization (CRBP) technique utilizing a water-soluble inibramer, sodium 2bromoacrylate. The green-light-induced atom transfer radical polymerization (ATRP) enabled the grafting of branched polymers directly from the protein surface in the open air. The resulting bioconjugates exhibited a predetermined molecular weight, well-defined architecture, and high branching density. Conformational analysis by SEC-MALS validated the controlled grafting of branched polymers. Furthermore, enzymatic assays revealed that densely grafted polymers prevented protein inhibitor penetration, and the resulting conjugates retained up to 90% of their enzymatic activity. This study demonstrates a promising strategy for designing protein−polymer bioconjugates with tunable sieving behavior, opening avenues for applications in drug delivery and biotechnology.

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