Dendrimer-encapsulated silver nanoparticles as a novel electrochemical label for sensitive immunosensors

Štofik, Marcel; Strýhal, Zdeněk; Malý, Jan

doi:10.1016/j.bios.2008.09.028

Cited by 36 publications

(10 citation statements)

References 25 publications

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“…Mali reported the synthesis and characterization of a novel electrochemical label for sensitive electrochemical stripping metallo-immunoassays based on silver den-drimer-encapsulated nanoparticles [165] . Several fixed ratios of Ag + /dendrimer were prepared with the aim of obtaining stable nanocomposites with maximal silver loading in the interior of a polymeric shell.…”

Section: Dendrimers: Dendrimers Are a Class Of Hyper Branchedmentioning

confidence: 99%

Molecular recognition by gold, silver and copper nanoparticles

Tauran¹,

Brioude²,

Coleman³

et al. 2013

WJBC

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The intrinsic physical properties of the noble metal nanoparticles, which are highly sensitive to the nature of their local molecular environment, make such systems ideal for the detection of molecular recognition events. The current review describes the state of the art concerning molecular recognition of Noble metal nanoparticles. In the first part the preparation of such nanoparticles is discussed along with methods of capping and stabilization. A brief discussion of the three common methods of functionalization: Electrostatic adsorption; Chemisorption; Affinity-based coordination is given. In the second section a discussion of the optical and electrical properties of nanoparticles is given to aid the reader in understanding the use of such properties in molecular recognition. In the main section the various types of capping agents for molecular recognition; nucleic acid coatings, protein coatings and molecules from the family of supramolecular chemistry are described along with their numerous applications. Emphasis for the nucleic acids is on complementary oligonucleotide and aptamer recognition. For the proteins the recognition properties of antibodies form the core of the section. With respect to the supramolecular systems the cyclodextrins, calix[n]arenes, dendrimers, crown ethers and the cucurbitales are treated in depth. Finally a short section deals with the possible toxicity of the nanoparticles, a concern in public health.

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Section: Dendrimers: Dendrimers Are a Class Of Hyper Branchedmentioning

confidence: 99%

Molecular recognition by gold, silver and copper nanoparticles

Tauran¹,

Brioude²,

Coleman³

et al. 2013

WJBC

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“…53,54 In this study, the PAMAM dendrimer was employed as a template to synthesize monodisperse Ag NPs according to a well-established method. 55 The dendrimer-encapsulated Ag (G5−Ag) was further reacted with Dex-CHO through the Schiff base linkage between surface primary amine groups of the PAMAM dendrimer and aldehyde groups on Dex-CHO, yielding the Dex-G5−Ag hydrogel (Figures 1 and S1). It is reported that bacterial growth generates acidic products such as carbonic acid and lactic acid during respiration and fermentation, yielding a mild acidic microenvironment.…”

Section: Resultsmentioning

confidence: 99%

“…Cationic dendrimers are a family of hyperbranched synthetic polymers with symmetrical topological architecture and abundant surface amine groups. − Compared to linear and branched polymers such as PEIs, dendrimers have a higher density of positive charges on the surface, which is beneficial for antibacterial activity. In addition, the globular shape and interior pockets of dendrimers enable them to be ideal templates for the synthesis of dendrimer-encapsulated metal NPs. , In this study, the PAMAM dendrimer was employed as a template to synthesize monodisperse Ag NPs according to a well-established method . The dendrimer-encapsulated Ag (G5–Ag) was further reacted with Dex-CHO through the Schiff base linkage between surface primary amine groups of the PAMAM dendrimer and aldehyde groups on Dex-CHO, yielding the Dex-G5–Ag hydrogel (Figures and S1).…”

Section: Results and Discussionmentioning

confidence: 99%

A Nanocomposite Hydrogel with Potent and Broad-Spectrum Antibacterial Activity

Dai

Wang

et al. 2018

ACS Appl. Mater. Interfaces

189

111

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Local bacterial infection is a challenging task and still remains a serious threat to human health in clinics. Systemic administration of antibiotics has only short-term antibacterial activity and usually causes adverse effects and bacterial resistance. A bioadhesive hydrogel with broad-spectrum and on-demand antibiotic activity is highly desirable. Here, we designed a pH-responsive nanocomposite hydrogel via a Schiff base linkage between oxidized polysaccharides and cationic dendrimers encapsulated with silver nanoparticles. The antibacterial components, both the cationic dendrimers and silver species, could be released in response to the acidity generated by growing bacteria. The released cationic polymer and silver exhibited a synergistic effect in antibacterial activity, and thus, the nanocomposite hydrogel showed potent antibacterial activity against both Gram-negative ( Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria ( Staphylococcus epidermidis and Staphylococcus aureus). The gel showed superior in vivo antibacterial efficacy against S. aureus infection compared with a commercial silver hydrogel at the same silver concentration. In addition, no obvious hemolytic toxicity, cytotoxicity, and tissue and biochemical toxicity were observed for the antibacterial hydrogel after incubation with cells or implantation. This study provides a facile and promising strategy to develop smart hydrogels to treat local bacterial infections.

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“…Today, most metal nanocomposites such as those of gold 9 , silver 10,11 , copper, nickel and mercury 12 are prepared in PAMAM solutions. By using the steric hindrance and coordination of PAMAM, longterm stable dispersions of silver nanocomposite with a narrow size distribution are produced easily and efficiently in PAMAM solutions 1,13,14 . But how long the silver nanocomposites will disperse steadily in the PAMAM solutions has not been reported, so far as the authors know.…”

Section: Introductionmentioning

confidence: 99%

The Stability of Ag/Polyamidoamine Nanocomposites in Aqueous Solutions

Peng

Zhao

Zhang

et al. 2015

Polymers and Polymer Composites

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Dendritic polyamidoamine (PAMAM) has been used as a template to synthesize metal nanocomposites, but the retention time of the nanocomposites in aqueous solutions has rarely been reported. In this paper, using the 5.5 generation of polyamidoamine (PAMAM) with ester-terminated groups as a template, the retention time for which the Ag ions were kept in the PAMAM aqueous solution was investigated by UV-Visible spectroscopy. It was found that the Ag/polyamidoamine nanocomposites showed high stability in the G5.5 solution even over 28 weeks. The results of TEM, TGA and DSC analyses also proved that smaller and more stable nanocomposites were formed in the solution. Thus, it can be concluded that the PAMAM may act as a good stabilizing agent for synthesizing Ag nanocomposites in aqueous solution.

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Dendrimer-encapsulated silver nanoparticles as a novel electrochemical label for sensitive immunosensors

Cited by 36 publications

References 25 publications

Molecular recognition by gold, silver and copper nanoparticles

Molecular recognition by gold, silver and copper nanoparticles

A Nanocomposite Hydrogel with Potent and Broad-Spectrum Antibacterial Activity

The Stability of Ag/Polyamidoamine Nanocomposites in Aqueous Solutions

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