Dendrimer-Functionalized Multi-walled Carbon Nanotubes Exhibit Dual-Phase Regulation to Exposed Murine Embryonic Stem Cells

Cui, Daxiang; Zhang, Hong; Wang, Zheng; Feng, Lili; Ruan, Jing; Asahi, Toru; Osaka, Tetsuya

doi:10.5101/nbe.v3i4.p227-231

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Effects of RGD-GNR-MWNT nanoprobes on viability of MGC803 and GES-1 cells were analyzed using Cell Counting Kit-8 (CCK8) assay [ 23 ]. MGC803 and GES-1 cells were cultured in a 96-well microplate at the concentration of 5,000 cells per well and incubated in a humidified 5% CO 2 balanced air incubator at 37°C for 24 h. Except for control wells, the remaining wells were added into the medium with RGD-GNR-MWNT nanoprobes.…”

Section: Methodsmentioning

confidence: 99%

RGD-conjugated silica-coated gold nanorods on the surface of carbon nanotubes for targeted photoacoustic imaging of gastric cancer

et al. 2014

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Herein, we reported for the first time that RGD-conjugated silica-coated gold nanorods on the surface of multiwalled carbon nanotubes were successfully used for targeted photoacoustic imaging of in vivo gastric cancer cells. A simple strategy was used to attach covalently silica-coated gold nanorods (sGNRs) onto the surface of multiwalled carbon nanotubes (MWNTs) to fabricate a hybrid nanostructure. The cross-linked reaction occurred through the combination of carboxyl groups on the MWNTs and the amino group on the surface of sGNRs modified with a silane coupling agent. RGD peptides were conjugated with the sGNR/MWNT nanostructure; resultant RGD-conjugated sGNR/MWNT probes were investigated for their influences on viability of MGC803 and GES-1 cells. The nude mice models loaded with gastric cancer cells were prepared, the RGD-conjugated sGNR/MWNT probes were injected into gastric cancer-bearing nude mice models via the tail vein, and the nude mice were observed by an optoacoustic imaging system. Results showed that RGD-conjugated sGNR/MWNT probes showed good water solubility and low cellular toxicity, could target in vivo gastric cancer cells, and obtained strong photoacoustic imaging in the nude model. RGD-conjugated sGNR/MWNT probes will own great potential in applications such as targeted photoacoustic imaging and photothermal therapy in the near future.

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Section: Methodsmentioning

confidence: 99%

RGD-conjugated silica-coated gold nanorods on the surface of carbon nanotubes for targeted photoacoustic imaging of gastric cancer

et al. 2014

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“…Up to date, many data showed that carbon nanotubes own cytotoxicity, and single walled carbon nanotubes exhibited stronger toxicity than multi-walled carbon nanotubes (MWCNTs) [10][11][12]. How to decrease carbon nanotubes' toxicity and enhance carbon nanotubes biocompatibility have become scientists' concerns, many methods have been tried [13].…”

Section: Introductionmentioning

confidence: 99%

Dendrimer Modified SWCNTs for High Efficient Delivery and Intracellular Imaging of Survivin SiRNA

Wang¹,

Li²,

Liu³

et al. 2013

Nano BioMed ENG

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Herein we reported that polyamidoamine dendrimer (PAMAM) modified single walled carbon nanotubes (PSWCNTs) were successfully used for high efficient delivery and intracellular imaging of survivin siRNA vector. No.4 generation of PAMAM dendrimers were used to modify single walled carbon nanotubes, and characterized by high resolution transmission electron microscopy (HR-TEM), and atom force microscopy (AFM), survivin shRNA vectors were constructed and identified, and then dendrimer-modified SWCNTs were mixed with survivin shRNA vectors, resultant dendrimer-SWCNTs-survivin shRNA vector mixtures co-cultured with human gastric cancer MGC803 cells, then the expression of survivin shRNA vectors in MGC803 cells was observed by fluorescent microscopy, cell viability was analyzed by MTT method. Results showed that survivin shRNA vector was successfully expressed in MGC803 cells, and MGC 803 cell growth was markedly inhibited. In conclusion, dendrimer (PAMAM) modified single walled carbon nanotubes can be used for high efficient delivery and intracellular imaging of siRNA, and own great potential in applications such as gene or drug delivery and tumor targeted imaging and simultaneous therapy in near future.

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Dendrimer-Functionalized Multi-walled Carbon Nanotubes Exhibit Dual-Phase Regulation to Exposed Murine Embryonic Stem Cells

Cited by 2 publications

References 32 publications

RGD-conjugated silica-coated gold nanorods on the surface of carbon nanotubes for targeted photoacoustic imaging of gastric cancer

RGD-conjugated silica-coated gold nanorods on the surface of carbon nanotubes for targeted photoacoustic imaging of gastric cancer

Dendrimer Modified SWCNTs for High Efficient Delivery and Intracellular Imaging of Survivin SiRNA

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