Dendritic cells derived from bone marrow and CD34<sup>+</sup> selected blood progenitor cells of myeloma patients, cultured in serum‐free media, do not contain the Kaposi sarcoma herpesvirus genome

Mitterer, Manfred; Mair, W.; Gatti, D; Sheldon, Julie; Vachula, M; Coser, P; Schultz, T. F.

doi:10.1046/j.1365-2141.1998.00894.x

Cited by 24 publications

(9 citation statements)

References 13 publications

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“…Furthermore, no statistically significant differences between MM patients and blood donors were observed in the frequency of HHV-8 seropositivity using an IFA assay 76 . Similar results have also been found by others [93][94][95][96][97] . Any significance of HHV-8 in the etiology of MM thus remains unclear.…”

Section: Multiple Myelomasupporting

confidence: 81%

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Leão¹,

Caterino–de–Araujo

Porter

et al. 2002

Rev. Hosp. Clin.

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OBJECTIVE: To review the current literature on human herpesvirus 8 with particular attention to the aspects related to the etiopathogenesis of Kaposi's sarcoma. MATERIALS AND METHODS: The authors searched original research and review articles on specific aspects of human herpesvirus 8 infection, including virology, epidemiology, transmission, diagnosis, natural history, therapy, and Kaposi's sarcoma etiopathogenesis. The relevant material was evaluated and reviewed. RESULTS: Human herpesvirus 8 is a recently discovered DNA virus that is present throughout the world but with major geographic variation. In the Western world, the virus, transmitted mainly by means of sexual contact, is strongly associated with Kaposi's sarcoma and body cavity-based lymphoma and more controversially with multiple myeloma and other non-proliferative disorders. There is no specific effective treatment, but HIV protease inhibitors may play an indirect role in the clearance of human herpesvirus 8 DNA from peripheral blood mononuclear cells of HIV-infected patients. Human herpesvirus 8 DNA is present in saliva, but there are as yet no documented cases of nosocomial transmission to health care workers. The prevalence of human herpesvirus 8 among health care workers is probably similar to that in the general population. CONCLUSION: Human herpesvirus 8 appears to be, at least in Western Europe and United States, restricted to a population at risk of developing Kaposi's sarcoma. Human herpesvirus 8 certainly has the means to overcome cellular control and immune responses and thus predispose carriers to malignancy, particularly Kaposi's sarcoma. The wide diffusion of Human herpesvirus 8 in classic Kaposi's sarcoma areas appears to represent an important factor in the high incidence of the disease. However, additional co-factors are likely to play a role in the development of Kaposi's sarcoma.

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Section: Multiple Myelomasupporting

confidence: 81%

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Leão¹,

Caterino–de–Araujo

Porter

et al. 2002

Rev. Hosp. Clin.

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“…Serologic studies have been almost uniform in their findings, demonstrating that patients with multiple myeloma lack antibodies to KSHV (44,188,200,224,256,290 This lack of reactivity is not due to generalized immunosuppression, since antibodies to other more ubiquitous herpesviruses, such as EBV, HHV-6, and cytomegalovirus, are easily detectable in these patients by using comparable methodologies. Since MGUS patients are known to develop multiple myeloma or other lymphoproliferative diseases within 10 to 20 years (152), the serum of MGUS patients, some of whom later developed multiple myeloma, was tested for the presence of HHV-8 IgG antibodies to both lytic and latent antigens (2).…”

Section: Multiple Myeloma and Hhv-8 Infectionmentioning

confidence: 98%

“…Multiple studies have been published since, with conflicting results. Several additional independent laboratories confirmed the specific presence of KSHV DNA sequences in multiple myeloma biopsies (4,60,238 However, other investigators have been unable to confirm this association when looking for KSHV DNA by PCR in bone marrow biopsies and/or dendritic cell cultures from bone marrow or peripheral blood (35,44,69,188,200,218,224,229,278,279,282,296).…”

Section: Multiple Myeloma and Hhv-8 Infectionmentioning

confidence: 99%

Spectrum of Kaposi's Sarcoma-Associated Herpesvirus, or Human Herpesvirus 8, Diseases

Ablashi¹,

Chatlynne²,

Whitman³

et al. 2002

Clin Microbiol Rev

278

182

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Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), discovered in 1994, is a human rhadinovirus (gamma-2 herpesvirus). Unlike other human herpesviruses (herpes simplex virus, Epstein-Barr virus, varicella-zoster virus, cytomegalovirus, HHV-6, and HHV-7), it is not widespread in the general population and has many unique proteins. HHV-8 is strongly associated with all subtypes of Kaposi's sarcoma (KS), multicentric Castleman's disease, and a rare form of B-cell lymphoma, primary effusion lymphoma. In addition, HHV-8 DNA sequences have been found in association with other diseases, but the role of the virus in these diseases is largely unconfirmed and remains controversial. The seroprevalence of HHV-8, based on detection of latent and lytic proteins, is 2 to 5% in healthy donors except in certain geographic areas where the virus is endemic, 80 to 95% in classic KS patients, and 40 to 50% in HIV-1 patients without KS. This virus can be transmitted both sexually and through body fluids (e.g., saliva and blood). HHV-8 is a transforming virus, as evidenced by its presence in human malignancies, by the in vitro transforming properties of several of its viral genes, and by its ability to transform some primary cells in culture. It is not, however, sufficient for transformation, and other cofactors such as immunosuppressive cytokines are involved in the development of HHV-8-associated malignancies. In this article, we review the biology, molecular virology, epidemiology, transmission, detection methods, pathogenesis, and antiviral therapy of this newly discovered human herpesvirus

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“…This finding was confirmed in some reports Chauhan et al, 1999) but not in others (MacKenzie et al, 1997;Parravicini et al, 1997;Mitterer et al, 1998). HHV8 encodes a homologue of the cytokine IL-6, known to be required for MM pathogenesis Burger et al, 1998), and expression of the viral IL-6 has been reported in dendritic cells of MM patients, providing a plausible hypothesis on a mechanism whereby HHV8 might be involved in MM pathogenesis.…”

mentioning

confidence: 72%

A prospective seroepidemiological study of human herpesvirus-8 infection and the risk of multiple myeloma

et al. 2001

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Presence of the Human Herpesvirus 8 (HHV8) genome has been reported in the bone marrow of multiple myeloma (MM) patients. So far, serological studies of HHV8 and MM have been inconsistent but have not included prospective epidemiological studies. We evaluated whether HHV8 infection is associated with increased risk for MM in a prospective population-based study of 39 000 Finnish subjects who donated serum samples in the period 1968–72. Serum samples from 47 subjects who developed MM during a 23-year follow-up and 224 age, area of residence and sex-matched subjects who remained healthy over a similar follow-up period were evaluated for HHV8 antibodies at enrolment, as assayed both with an immunofluorescence assay (IFA) for lytic and latent HHV8 antigens and by Western blot (WB) with three recombinant HHV8 proteins (ORFs 65, 73 and K8.1A). HHV8 seropositivity for at least one HHV8 protein on WB was found in 7% of the Finnish population and was not associated with the risk of developing MM (Relative Risk (RR) = 0.89, Confidence Interval (CI): 0.25–3.25). HHV8 seropositivity for lytic and latent antigens in the IFA was found in 16% and 0.4% of the Finnish population and tended to associate with risk of MM (RR = 2.02, CI: 0.94–4.33 and RR = 10.00, CI: 0.91–110.29, respectively). In conclusion, no statistically significant evidence for an association between HHV8 infection and the risk of future MM was found. © 2001 Cancer Research Campaign http://www.bjcancer.com

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Dendritic cells derived from bone marrow and CD34⁺ selected blood progenitor cells of myeloma patients, cultured in serum‐free media, do not contain the Kaposi sarcoma herpesvirus genome

Cited by 24 publications

References 13 publications

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Spectrum of Kaposi's Sarcoma-Associated Herpesvirus, or Human Herpesvirus 8, Diseases

A prospective seroepidemiological study of human herpesvirus-8 infection and the risk of multiple myeloma

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Dendritic cells derived from bone marrow and CD34+ selected blood progenitor cells of myeloma patients, cultured in serum‐free media, do not contain the Kaposi sarcoma herpesvirus genome

Cited by 24 publications

References 13 publications

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Spectrum of Kaposi's Sarcoma-Associated Herpesvirus, or Human Herpesvirus 8, Diseases

A prospective seroepidemiological study of human herpesvirus-8 infection and the risk of multiple myeloma

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Product

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Dendritic cells derived from bone marrow and CD34⁺ selected blood progenitor cells of myeloma patients, cultured in serum‐free media, do not contain the Kaposi sarcoma herpesvirus genome