Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments

Immunogenicity is preferentially induced in sparse dendritic cell cultures

“…On the other hand, MycL and Irf4 , which modulate T cell priming and the induction of different helper T cell lineages by DCs192021, were expressed at higher levels in sparse cultures indicating potentially unique features of antigen presentation in the two DC types. This hypothesis was further supported by the accumulation of a diverse set of mediators involved in immunosuppressive mechanisms among the genes highly expressed in dense DC cultures222324252627282930, including Lair1 , Arg1 , Vegfa , Igf1 , Stab1 , Pf4 , Nt5e , Cd276 , Cd200 (Fig. 1g).…”

Activation of dendritic cells by crosslinked collagen hydrogels (artificial corneas) varies with their composition

“…Similarly, the fabricated matrices studied here are probably tolerogenic, but variably so, depending on the respective gel chemistry. Soluble collagen bound to tissue culture plastic is known to be non‐activating for DC (Bayrak et al, ), and this is mediated via the collagen receptor LAIR‐1 (Leslee Sprague et al, ). In the present study, both the natural matrices and the engineered collagen gels induced upregulation of LAIR‐1, although to different degrees (Figure a).…”

The role of collagen in cancer: from bench to bedside

“…However, COLXVII overexpression in cancer cells by activated leukocyte-associated immunoglobulin-like receptor-1 diminished natural killer cell cytotoxic activity [144]. By interaction with collagen in ovarian and breast cancer, tumor-associated dendritic cells expressed the highly receptor CD305/leukocyte-associated immunoglobulin-like receptor-1 [145].…”