Dendritic eIF4E-binding Protein 1 (eIF4E-BP1) mRNA Is Upregulated by Neuronal Activation

Moon, Il Soo; Lee, Hyung Jong; Park, In Sick

doi:10.3346/jkms.2012.27.10.1241

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…Fatty acid binding proteins (FABPs) play a critical role as their cellular shuttles [ 26 ]. EIF4E is a signaling protein that regulates cell growth and proliferation, and can be activated in dendrites due to the activation of neurons [ 27 ]. MSRB3 is involved in protein–protein interactions, including protein folding, neuroprotection, and cell survival [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Markers in the Muscles and Brain of Pigs Recovered from Hemorrhagic Stroke

Fedulova

Vasilevskaya

Tikhonova

et al. 2022

Genes

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(1) Background: Stroke is the leading cause of serious long-term disability. Walking dysfunction and paresis of the upper extremities occurs in more than 80% of people who have had a stroke. (2) Methods: We studied post-genomic markers in biosamples of muscle and brain tissue from animals that underwent intracerebral hematoma and recovered after 42 days. Our purpose was to understand the biological mechanisms associated with recovery from hemorrhagic stroke. We analyzed the peptides formed after trypsinolysis of samples by HPLC-MS, and the results were processed by bioinformatics methods, including the establishment of biochemical relationships (gene to gene) using topological omics databases such as Reactome and KEGG. (3) Results: In the pig brain, unique compounds were identified which are expressed during the recovery period after traumatic injury. These are molecular factors of activated microglia, and they contribute to the functional recovery of neurons and reduce instances of hematoma, edema, and oxidative stress. Complexes of the main binding factors of the neurotrophins involved in the differentiation and survival of nerve cells were found in muscles. (4) Conclusions: A network of gene interactions has been constructed for proteins involved in the regulation of synaptic transmission, in particular presynaptic vesicular and endocytic processes. The presence of transmitters and transporters associated with stimulation of NMDA receptors at neuromuscular junctions shows the relationship between upper motor neurons and neuromuscular junctions.

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Section: Discussionmentioning

confidence: 99%

Proteomic Markers in the Muscles and Brain of Pigs Recovered from Hemorrhagic Stroke

Fedulova

Vasilevskaya

Tikhonova

et al. 2022

Genes

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“…It was reported that 4EBP1 protein and its mRNA are upregulated and transported to dendritic domains in RNA granules upon neuronal activation 33 . Local protein synthesis in axons is a critical component of synaptic plasticity.…”

Section: Discussionmentioning

confidence: 99%

Neural-specific deletion of mitochondrial p32/C1qbp leads to leukoencephalopathy due to undifferentiated oligodendrocyte and axon degeneration

Yagi

Uchiumi

Sagata

et al. 2017

Sci Rep

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Mitochondrial dysfunction is a critical step in the pathogenesis of many neurodegenerative diseases. The p32/ C1qbp gene functions as an essential RNA and protein chaperone in mitochondrial translation, and is indispensable for embryonic development. However, little is known about the consequences of mitochondrial dysfunction of p32 deletion in the brain development. Here, we found that mice lacking p32 in the central nervous system (p32cKO mice) showed white matter degeneration accompanied by progressive oligodendrocyte loss, axon degeneration and vacuolation in the mid brain and brain stem regions. Furthermore, p32cKO mice died within 8 weeks of birth. We also found that p32-deficient oligodendrocytes and neurons showed reduced oligodendrocyte differentiation and axon degeneration in primary culture. We show that mitochondrial disruption activates an adaptive program known as the integrated stress response (ISR). Mitochondrial respiratory chain function in oligodendrocytes and neurons is, therefore, essential for myelination and axon maintenance, respectively, suggesting that mitochondrial respiratory chain dysfunction in the central nervous system contributes to leukoencephalopathy.

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“…This result was in accordance with the previous report. DIV 16 hippocampal neurons expressed eIF4E throughout dendrites but not in the nucleus, while eIF4EBP1 was distributed throughout the neuron with highest expression in the nucleus [9]. There were many eIF4E, eIF4EBP1 and PSD95 puncta in the dendrites of DIV 20 neurons (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…High resolution confocal microscopy and detergent extraction experiments showed that the eIF4E, eIF4G, eIF5, eIF5A and eIF6 were localized to the postsynaptic sites in association with the postsynaptic density (PSD) [3]. Furthermore, eIF4E and eIF4EBP1 mRNAs are localized, and upregulated by neuronal stimulation, in dendrites of cultured rat hippocampal neurons [9,11].Neurons undergo complex morphological development. Dendrites will initially outgrow, followed by extensive branching and lengthening during dendrogenic stage.…”

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confidence: 99%

Developmental Expression of Eukaryotic Initiation Factor 4E (eIF4E) and eIF4E-binding Protein 1 (eIF4EBP1) in Rat Hippocampal Neurons

Park¹,

Moon²

2013

Journal of Life Science

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Local protein synthesis at subsynaptic sites plays a key role in the regulation of the protein composition in local domains. In this study, we carried out immunocytochemistry of cultured rat hippocampal neurons in various developmental stages to investigate the expression of eIF4E and its binding protein, eIF4EBP1. Both proteins were distributed in dendrites. In addition, eIF4EBP1 was highly expressed in the nucleus throughout the development, whereas eIF4E was not expressed in the nucleus. Punctate expression of eIF4E and eIF4EBP1 was evident in DIV 3. The colocalization rates of eIF4E or eIF4EBP1 puncta with PSD95 were higher in the dendrogenic than in the mature stages. In contrast, the colocalization rates of eIF4E and eIF4EBP1 puncta were higher in the mature than in the dendrogenic stages. As eIF4E is inactive when it is bound to eIF4EBP1, these data indicate that most dendritic eIF4E's are active during development but that they are mostly under inhibition in mature neurons.Key words : Development, eukaryotic translation initiation factor 4E (eIF4E), eIF4E-binding protein 1 (eIF4EBP1), hippocampal neuron, immunocytochemistry † Present address: Seoul Yonhap Clinic, 69-48 Namseok-Ri, YeongdeokEup, Yeongdeok-Gun, Gyeongsangbuk-Do 766-801, Korea *Corresponding author *Tel：+82-54-770-2414, Fax：+82-54-770-2447 *E-mail : moonis@dongguk.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. IntroductionFunctions of the brain are based on the neural networks which are dynamically remodeled by changes in the strength of synaptic connections. The long-lasting changes in synaptic strength, such as long-term potentiation (LTP), require new protein synthesis [4,8,13]. The local protein synthesis at subsynaptic sites plays a key advantageous mechanism that would regulate the protein composition in local domains on a moment-by-moment basis. In the translation process of protein synthesis, initiation is the rate limiting step where most of translational control occurs [14]. During translation initiation, eukaryotic translation initiation factor 4E (eIF4E) binds the 5'-cap structure of mRNA and the ribosome-associated scaffold protein eIF4G [17] in the cytoplasm. The eIF4E-binding protein 1 (eIF4EBP1) acts as a negative regulator of this process by preventing interaction of eIF4E with eIF4G in a competitive manner [7].In addition to the organelles such as rough endoplasmic reticulum and Golgi outpost that are necessary for protein synthesis, many translation factors were found located in the dendrite. Presence of eIF4E in postsynaptic region was revealed by electron microscopic study [1]. Immunocytochemistry (ICC) and western blotting also showed the postsynaptic localization of eIF4E, eIF4EBP1, eIF4EBP2 [16]. High resolution confocal microscopy and detergent extraction ...

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Dendritic eIF4E-binding Protein 1 (eIF4E-BP1) mRNA Is Upregulated by Neuronal Activation

Cited by 6 publications

References 33 publications

Proteomic Markers in the Muscles and Brain of Pigs Recovered from Hemorrhagic Stroke

Proteomic Markers in the Muscles and Brain of Pigs Recovered from Hemorrhagic Stroke

Neural-specific deletion of mitochondrial p32/C1qbp leads to leukoencephalopathy due to undifferentiated oligodendrocyte and axon degeneration

Developmental Expression of Eukaryotic Initiation Factor 4E (eIF4E) and eIF4E-binding Protein 1 (eIF4EBP1) in Rat Hippocampal Neurons

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