Dendrobium micropropagation: a review

Silva, Jaime A. Teixeira da; Cardoso, Jean Carlos; Dobránszki, Judit; Zeng, Songjun

doi:10.1007/s00299-015-1754-4

Cited by 68 publications

(45 citation statements)

References 75 publications

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“…In this medium, periodic subcultures (4-5 times, each subculture period was 15 days) were carried out in the same medium to proliferate PLBs. For more details, also see Teixeira da Silva et al (2015a) and Teixeira da Silva and Winarto (2016). Fourth, bacterial (I-O) and fungal (P-U) contamination of Dendrobium 'Zahra FR 62' (I, J, L, M, O, R), D. 'Gradita 31' (K, N, P, S) and D. 'Jayakarta' (Q, T) in solid and semi-solid cultures of shoot tips (I-K) or PLBs (L-T) can take place 2 months (I-Q) or 1.5 months (R-T) after culture initiation on half-strength MS containing 0.3 mg dm -3 TDZ and 0.1 mg dm -3 NAA.…”

Section: Choice Of Methods For Surface Disinfectionmentioning

confidence: 99%

“…Using these principles, this review seeks to find how disinfection procedures have been used to prepare in vivo-derived plant material for in vitro culture in Dendrobium since the in vitro environment serves as an important tool for multiple biotechnological advances, symbiotic and asymbiotic seed germination, and molecular advances, including genetic transformation (Teixeira da Silva et al 2015aSilva et al , 2015bSilva et al , 2015c.…”

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confidence: 99%

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Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), the induction, regeneration or multiplication of shoots, and the preparation and proliferation of plantlets suitable for acclimatization. The initiation of an aseptic culture is closely related to the appropriate selection of an explant source and its preparation, including its (in vivo) pre-treatment if necessary and subsequent disinfection procedures. Care in the choice of explant and the application of an appropriate disinfection protocol can successfully reduce, or eliminate, contamination in in vitro cultures while reducing the negative impact on plant tissues and plantlet regeneration. Many unique aseptic culture procedures for Dendrobium genus have been reported in the literature, very often specific to particular tissues or genotypes, and this review not only highlights the details of such protocols, but also provides practical advice for novice -and even seasoned -orchidologists who wish to research Dendrobium in vitro, although it is cautioned that there is currently no universal aseptic culture procedure that can be applied to all conditions, all explants or all genotypes.

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Section: Choice Of Methods For Surface Disinfectionmentioning

confidence: 99%

mentioning

confidence: 99%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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“…These propagation characteristics, in addition to ensuring the quality of the plantlets produced, also aim to maintain the commercial scale necessary to meet the target market. The only viable technique that combines all these characteristics has been in vitro micropropagation of orchids [17].…”

Section: Introductionmentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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The process through induction, proliferation and regeneration of protocorm-like bodies (PLBs) is one of the most advantageous methods for mass propagation of orchids which applied to the world floricultural market. In addition, this method has been used as a tool to identify genes of interest associated with the production of PLBs, and also in breeding techniques that use biotechnology to produce new cultivars, such as to obtain transgenic plants. Most of the molecular studies developed have used model plants as species of Phalaenopsis, and interestingly, despite similarities to somatic embryogenesis, some molecular differences do not yet allow to characterize that PLB induction is in fact a type of somatic embryogenesis. Despite the importance of species for conservation and collection purposes, the flower market is supported by hybrid cultivars, usually polyploid, which makes more detailed molecular evaluations difficult. Studies on the effect of plant growth regulators on induction, proliferation, and regeneration of PLBs are the most numerous. However, studies of other factors and new technologies affecting PLB production such as the use of temporary immersion bioreactors and the use of lighting-emitting diodes have emerged as new tools for advancing the technique with increasing PLB production efficiency. In addition, recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation, such as somaclonal variation.

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“…Chromosome manipulation is an effective way of orchid improvement (Hossain et al, 2013). Because orchid seeds produce protocorm during germination, and because in vitro regeneration induces protocorm-like bodies (PLBs) (Morel, 1960;Teixeira da Silva et al, 2015), protocorms or PLBs can be easily treated with colchicine for manipulation of chromosomes (Yang et al, 2013;Zhan and Cheng, 2011). PLBs are composed of many meristematic centers that differentiate into shoots and roots (Cui et al, 2008).…”

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Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

Pham¹,

Li²,

Guo³

et al. 2019

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Dendrobium officinale Kimura et Migo is a famous traditional Chinese medicinal plant. It produces various phytochemicals, particularly polysaccharides, which have nutraceutical and pharmaceutical values. To increase its biomass production and polysaccharide content, our breeding program has generated a series of polyploid cultivars through colchicine treatment of protocorm-like bodies (PLBs). The present study compared two tetraploid cultivars, 201-1-T1 and 201-1-T2, with their diploid parental cultivar, 201-1, in an established in vitro culture system. Tetraploid ‘201-1-T1’ and ‘201-1-T2’ had shorter leaves and shorter and thicker stems and roots, and they produced higher biomass compared with the diploid cultivar. The length and width of stomata significantly increased, but stomatal density decreased in tetraploid cultivars. The PLB induction rates from the stem node explants of the tetraploid cultivars were significantly higher than those of diploid. However, the PLB proliferation of tetraploids was lower than that of the diploid. The mean number of plantlets regenerated from tetraploid PLBs was also lower than that of the diploid after 4 months of culture. Polysaccharide contents in stems, leaves, and roots of 6-month-old tetraploid plantlets were significantly higher than those of diploids. The polysaccharide content in the stem of ‘201-1-T1’ was 12.70%, which was a 2-fold increase compared with the diploid cultivar. Our results showed that chromosome doubling could be a viable way of improving D. officinale in biomass and polysaccharide production.

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Dendrobium micropropagation: a review

Cited by 68 publications

References 75 publications

Tissue disinfection for preparation of Dendrobium in vitro culture

Tissue disinfection for preparation of Dendrobium in vitro culture

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

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