Dendronized Chitosan Derivative as a Biocompatible Gene Delivery Carrier

Deng, Junjie; Zhou, Yanfang; Xu, Bo; Mai, Kaijin; Deng, Yong; Zhang, Liming

doi:10.1021/bm101303f

Cited by 72 publications

(42 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16,29,31 As shown in Figure 6A, the zeta potential of the glycogen derivative/pDNA complexes increased with increasing weight ratios. After the complexes formed, their zeta potentials became positive, which is amenable to the effective condensation of DNA.…”

Section: Analysis Of Zeta Potentials and Particle Sizesmentioning

confidence: 84%

“…4,16 Each sample solution (0.2 mg/mL) was prepared in 30 mL of an aqueous NaCl solution (0.15 mol/ mL). The sample solutions were initially titrated with a 0.1 mol/mL NaOH solution to a pH of 10.0.…”

Section: Buffer Capacitymentioning

confidence: 99%

“…11 Following amination, various cationized polysaccharide derivatives have been designed and synthesized as gene-delivery vectors, with high transfection efficiency. 3,[11][12][13][14][15][16][17][18] Glycogen is a storage form of glucose that occurs predominantly in the liver and skeletal muscles of mammalian and shellfish species. 19 It is a natural hyperbranched polysaccharide with high weight-average molecular weight: about 10 6 -10 7 g/mol.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Liu¹,

Ren²,

Liu³

et al. 2014

IJN

Self Cite

View full text Add to dashboard Cite

Background The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector. Methods A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy. Their buffer capacity was assessed by acid–base titration in aqueous NaCl solution. Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis. The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy. Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively. pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines. In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency. Results The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized. They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI). They were able to bind and condense pDNA to form the complexes of 100–250 nm in size. The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI. Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group. Conclusion The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.

show abstract

Section: Analysis Of Zeta Potentials and Particle Sizesmentioning

confidence: 84%

Section: Buffer Capacitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Liu¹,

Ren²,

Liu³

et al. 2014

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…The design of dendronized-based hybrid material is appealing in order to combine unique features of both materials. For instance, dendronized polymers based on polyamidoamine (PAMAM) and chitosan (a natural cationic organic polymer) as the core were synthesized and used as a biocompatible gene delivery vector [86]. In line with this concept, Tehrani and Basiryan were able to covalently graft PAMAM up to three generations on the surface of CNCs by a divergent method [87].…”

Section: Cationization Of Cncs Via Grafting Of Cationic Hyperbranchedmentioning

confidence: 99%

Synthetic Strategies for the Fabrication of Cationic Surface-Modified Cellulose Nanocrystals

Sunasee

Hemraz

2018

Fibers

View full text Add to dashboard Cite

Cellulose nanocrystals (CNCs) are renewable nanosized materials with exceptional physicochemical properties that continue to garner a high level of attention in both industry and academia for their potential high-end material applications. These rod-shaped CNCs are appealing due to their non-toxic, carbohydrate-based chemical structure, large surface area, and the presence of ample surface hydroxyl groups for chemical surface modifications. CNCs, generally prepared from sulfuric acid-mediated hydrolysis of native cellulose, display an anionic surface that has been exploited for a number of applications. However, several recent studies showed the importance of CNCs' surface charge reversal towards the design of functional cationic CNCs. Cationization of CNCs could further open up other innovative applications, in particular, bioapplications such as gene and drug delivery, vaccine adjuvants, and tissue engineering. This mini-review focuses mainly on the recent covalent synthetic methods for the design and fabrication of cationic CNCs as well as their potential bioapplications.

show abstract

“…Chitosan has primary amine groups and these repeating groups become protonated in acidic conditions. Chitosan derivatives have better solubility and buffering capacities [52]. This property of chitosan derivatives has efficiently been used to transfect different types of cells lines for delivering genes.…”

Section: Chitosan In Gene Deliverymentioning

confidence: 99%

Chitosan Nanoparticles for Generating Novel Systems for Better Applications: A Review

G¹,

KS²

2015

J Mol Genet Med

View full text Add to dashboard Cite

The environmental sustainability and use of environmentally acceptable biomaterials are major areas of scientific and industrial research. In recent times, identification and application of suitable biopolymers instead of petroleum derived conventional polymers are gaining more attention. Chitosan and chitosan derived nanoparticles and nanocomposites are very promising due to their versatile properties and have found applications in various areas such as biomedical, foods, food packaging and personal care. Many studies suggest that chitosan nanoparticles and their derivatives are one of the best barrier and film coating materials for preserving the quality of foods and for delivering skin care products, mainly due to their biodegradability and anti-microbial properties. The present review gives insight on the progress made in the application of chitosan nanoparticles specifically in foods and food packaging, personal care products and in biomedical industry. Future studies shall address establishment of industrial scale up of chitosan nanoparticles, safety and quality aspects of these chitosan derived products for human use.

show abstract

Dendronized Chitosan Derivative as a Biocompatible Gene Delivery Carrier

Cited by 72 publications

References 40 publications

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Synthetic Strategies for the Fabrication of Cationic Surface-Modified Cellulose Nanocrystals

Chitosan Nanoparticles for Generating Novel Systems for Better Applications: A Review

Contact Info

Product

Resources

About