Denervation and repeated ?-DOPA induce a coordinate expression of the transcription factor NGFI-B in striatal projection pathways in hemi-parkinsonian rats

Nur77 Gene Knockout Alters Dopamine Neuron Biochemical Activity and Dopamine Turnover

“…On the other hand, Nur77 and Nor-1 are highly expressed in target areas of DA neurons, such as the striatal complex and prefrontal cortex (Beaudry et al 2000;Werme et al 2000a;Zetterström et al 1996). In these areas, strong modulation of Nur77 and Nor-1 has been observed after manipulation of DA neurotransmission with DA receptor antagonists (neuroleptics) or psychostimulants, or after DA denervation (Beaudry et al 2000;Ethier et al 2004a;St-Hilaire et al 2003a;2003b;Werme et al 2000a;2000b). In addition, we have shown that Nur77-deficient mice had an altered response after treatment with antipsychotic drugs (Ethier et al 2004a;2004b).…”

Nur77 mRNA levels and L-Dopa-induced dyskinesias in MPTP monkeys treated with docosahexaenoic acid

“…The double in situ hybridization procedure was performed as previously described (Beaudry et al, 2000;St-Hilaire et al, 2003). Briefly, double in situ hybridization with a [ 35 S]UTPlabeled Nur77 and a non-radioactive dioxygenin (Dig)-labeled preprotachykinin, which produces substance P (SP), was performed (Krause et al, 1987).…”

“…Indeed, chronic Fos proteins of the ΔFosB family of transcription factors in the caudate-putamen, which, when coupled with Jun-D, form AP-1 complexes that can modulate the expression of several genes associated with the generation of LIDs in rodent and primate models of PD (Andersson et al, 1999;Doucet et al, 1996;Pavon et al, 2006). In recent years, we have shown that an orphan transcription factor of the nuclear receptor family, namely Nur77 (also known as Nerve-Growth Factor Inducible gene B (NGFI-B) or NR4A1) might also be associated with movement disorders in rodent models of PD (Sgambato-Faure et al, 2005;St-Hilaire et al, 2005;St-Hilaire et al, 2003;van den Munckhof et al, 2006).…”

“…Its expression is strongly modulated after manipulation of dopamine neurotransmission, for review see (Lévesque and Rouillard, 2007). Unilateral denervation induced by local injection of 6-hydroxydopamine (6-OHDA) in rats produces a complex regulation of Nur77 in the striatum (St-Hilaire et al, 2005;St-Hilaire et al, 2003). The expression of Nur77 transcripts is selectively up-regulated in enkephalin (ENK)-containing cells of the indirect striatal output pathway, whereas Nur77 expression is reduced in dynorphin (DYN)-positive cells of the direct output pathway in the denervated striatum (StHilaire et al, 2005;St-Hilaire et al, 2003).…”

“…Unilateral denervation induced by local injection of 6-hydroxydopamine (6-OHDA) in rats produces a complex regulation of Nur77 in the striatum (St-Hilaire et al, 2005;St-Hilaire et al, 2003). The expression of Nur77 transcripts is selectively up-regulated in enkephalin (ENK)-containing cells of the indirect striatal output pathway, whereas Nur77 expression is reduced in dynorphin (DYN)-positive cells of the direct output pathway in the denervated striatum (StHilaire et al, 2005;St-Hilaire et al, 2003). Interestingly, subsequent chronic L-DOPA treatment further reduced Nur77 expression in DYN-positive cells in specific striatal areas, whereas it strongly increased Nur77 mRNA levels in this same cell subpopulation in the non-denervated striatum (St-Hilaire et al, 2005;St-Hilaire et al, 2003).…”