Dengue-4 false negative results by Panbio® Dengue Early ELISA assay in Brazil

Colombo, Tatiana Elias; Vedovello, Danila; Araki, Carlos Shigueru; Cogo‐Moreira, Hugo; Santos, Izalco Nuremberg Penha dos; Reis, Andréia Francesli Negri; Costa, Fabiana Rodrigues; Cruz, Lilian Elisa Arão Antônio; Casagrande, Liane; Regatieri, Livia José; Junior, Jurandir Ferreira; Bronzoni, Roberta Vieira de Morais; Schmidt, Diane; Nogueira, Maurício Lacerda

doi:10.1016/j.jcv.2013.10.021

Cited by 18 publications

(23 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These patient samples were collected from 17 countries in Latin America, Asia and Oceania, and the most studies were conducted in Brazil (27%) [29], [34], [37], [41], [45], [47], [52], [54], Vietnam (13%) [32], [33], [36], [49], Malaysia (13%) [26], [36], [42], [46] and Thailand (13%) [31], [36], [43], [48]. Regarding the design of the studies, they were classified into two types: prospective and retrospective cohorts, of which only twelve reported that their samples were collected during dengue outbreaks [25], [26], [30], [37], [39], [40], [44], [45], [51]–[54].…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the design of the studies, they were classified into two types: prospective and retrospective cohorts, of which only twelve reported that their samples were collected during dengue outbreaks [25], [26], [30], [37], [39], [40], [44], [45], [51]–[54]. Typically, most samples were collected until the sixth day of the onset of symptoms [25], [27], [28], [30]–[37], [40]–[42], [47], [49], [50], [52]–[54]. The data extracted from the final selection are shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…Among the selected studies, 16 assessed the trials of Panbio [27], [28], [30], [35]–[41], [45], [47], [48], [50]–[52] and 23 assessed the trials of Platelia [25]–[27], [29]–[39], [42]–[44], [46], [47], [49], [50], [53], [54]. In relation to Panbio, the sensitivity, specificity, LR+, LR- and DOR overall were 66% (95% CI 61–71), 99% (95% CI 96–100), 98 (95% CI 19–367), 0.3 (95% CI 0.2–0.4) and 289 (95% CI 59–1412), respectively.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

A Meta-Analysis of the Diagnostic Accuracy of Two Commercial NS1 Antigen ELISA Tests for Early Dengue Virus Detection

2014

View full text Add to dashboard Cite

BackgroundDengue virus (DENV) NS1 antigen detection is regarded as an early diagnostic marker. Accordingly, several studies have evaluated the performance of tests that utilize NS1 capture, but the results of individual studies may be limited due to the restricted sample size of the patients recruited. Therefore, our objective was to perform a meta-analysis of the diagnostic accuracy of two commercial NS1 ELISAs (Panbio and Platelia).Methods and ResultsStudies of interest were found in PubMed, Embase and Google Scholar databases using defined inclusion/exclusion criteria. A total of 30 studies containing 12,105 total enrolled patients were included. The results were as follows: 1) Panbio assays showed low overall performance, sensitivity 66% (95% confidence interval (CI) 61–71), specificity 99% (95% CI 96–100), positive likelihood ratio (LR+) 98 (95% CI 20–464), negative likelihood ratio (LR-) 0.3 (95% CI 0.2–0.4), diagnostic odds ratio (DOR) 289 (95% CI 59–1412); 2) Platelia assays showed high overall performance, sensitivity 74% (95% CI 63–82), specificity 99% (95% CI 97–100), LR+ 175 (95% CI 28–1099), LR- 0.3 (95% CI 0.2–0.4), DOR 663 (95% CI 98–4478). The lowest sensitivity values were for secondary infections (57% [95% CI 47–67] and 66% [95% CI 53–77] for Panbio and Platelia, respectively) and for the detection of DENV4. Regarding clinical manifestations, the sensitivity of Platelia was 69% (95% CI 43–86) and 60% (95% CI 48–70) for fever and dengue hemorrhagic fever, respectively. In addition, the sensitivity of both tests was slightly lower for samples from Southeast Asia and Oceania.ConclusionDENV1 samples gave higher sensitivity results for both tests. We observed that factors negatively influencing the tests, such as the type of infection, geographical origins of samples and viral serotypes, require further investigation to optimize the diagnostic accuracy.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A Meta-Analysis of the Diagnostic Accuracy of Two Commercial NS1 Antigen ELISA Tests for Early Dengue Virus Detection

2014

View full text Add to dashboard Cite

show abstract

“…Since 2009, there was a reintroduction of DENV-1, followed by explosive outbreaks of DENV-1 and DENV-4 with about 20,000 suspected cases per year and the co-circulation of three serotypes. On the other hand, DENV-3 caused the major epidemics in 2006 and 2007, followed by a DENV-2 epidemic in 2008 [36–39]. Due to insufficient in the database, it was not possible to correlate the data from TTs with serotypes specifically, but the association between serotypes and genotypes may be related to this phenomenon.…”

Section: Discussionmentioning

confidence: 99%

Low sensitivity of the tourniquet test for differential diagnosis of dengue: an analysis of 28,000 trials in patients

et al. 2016

Self Cite

View full text Add to dashboard Cite

BackgroundThe aim of this study was to evaluate the utility of the tourniquet test (TT) for dengue diagnosing. To our knowledge, no previous study with such a large sample, of this duration, with as many laboratory methods referenced, or relating the results of the TT to the 2009 WHO classification of severity has been conducted thus far.MethodsIn this study, we analyzed the records of 119,589 suspected dengue cases in a Brazilian city, with 30,670 confirmed cases. The Cohen’s Kappa test was applied to evaluate the degree of agreement between the tests, and the sensitivity and specificity was calculated for the TT.ResultsTwenty-eight thousand six hundred thirty-five TT were performed. No association between the outcome of the TT and greater severity of infection, according to the 2009 guideline, was observed (P = 0.28); furthermore, relevant agreement with the final diagnosis (κ = 0.01; 95 % CI = 0.00 to 0.02) or individually with the IgM enzyme-linked immunoassay was not observed (κ = 0.05; 95 % CI = 0.04 to 0.06), and was even lower with PCR (κ = 0.27; 95 % CI = 0.06 to 0.49). Most importance of the TT was shown in relation to specificity (88.9 %; 95 % CI = 0.88 to 0.89) and negative predictive value (70.3 %; CI 95 % = 0.70 to 0.71).ConclusionsTT was more effective in detecting cases that were truly negative than positive. These results suggest that the TT should not be used as diagnosis of dengue.

show abstract

“…13,14 NS1 tests are also reported to be less sensitive in secondary dengue. 3,12,15 Little is known about the causes of false-positive NS1 tests, except for possible cross-reactivity with other flaviviruses and possibly, cytomegalovirus (CMV).…”

Section: Commentmentioning

confidence: 99%

Two Cases of False-Positive Dengue Non-Structural Protein 1 (NS1) Antigen in Patients with Hematological Malignancies and a Review of the Literature on the Use of NS1 for the Detection of Dengue Infection

Chung¹,

Krishnan²,

Leo³

2015

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Early diagnosis of dengue has been made easier in recent years owing to the advancement in diagnostic technologies. The rapid non-structural protein 1 (NS1) test strip is widely used in many developed and developing regions at risk of dengue. Despite the relatively high specificity of this test, we recently encountered two cases of false-positive dengue NS1 antigen in patients with underlying hematological malignancies. We reviewed the literature for causes of falsepositive dengue NS1.

show abstract

Dengue-4 false negative results by Panbio® Dengue Early ELISA assay in Brazil

Cited by 18 publications

References 19 publications

A Meta-Analysis of the Diagnostic Accuracy of Two Commercial NS1 Antigen ELISA Tests for Early Dengue Virus Detection

A Meta-Analysis of the Diagnostic Accuracy of Two Commercial NS1 Antigen ELISA Tests for Early Dengue Virus Detection

Low sensitivity of the tourniquet test for differential diagnosis of dengue: an analysis of 28,000 trials in patients

Two Cases of False-Positive Dengue Non-Structural Protein 1 (NS1) Antigen in Patients with Hematological Malignancies and a Review of the Literature on the Use of NS1 for the Detection of Dengue Infection

Contact Info

Product

Resources

About