Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

Velandia-Romero, Myriam L.; Olano, Víctor Alberto; Coronel-Ruiz, Carolina; Cabezas, Laura; Castellanos, Jaime E.; Mátiz, María Inés

doi:10.7705/biomedica.v37i0.3584

Cited by 13 publications

(6 citation statements)

References 20 publications

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“…The laboratory analysis conducted in this study revealed the presence of DENV serotypes 1 and 2 in mosquitoes collected from the 24 de diciembre and Serranía neighborhoods. This finding is consistent with previous entomo-virological studies conducted in Colombia, where all four DENV serotypes have been detected, with serotypes 1 and 2 being the most predominant [ 6 , 30 , 32 , 52 ]. Although the infection rates observed in this study were relatively low, with a minimum infection rate of 1.4 and a positivity rate of 1.2%, the high density and large number of infested water reservoirs indicate a significant risk of DENV transmission in the study areas.…”

Section: Discussionsupporting

confidence: 92%

Density of Aedes aegypti and dengue virus transmission risk in two municipalities of Northwestern Antioquia, Colombia

Gómez-Vargas,

Ríos-Tapias,

Marin-Velásquez

et al. 2024

PLoS ONE

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The high infestation of Aedes aegypti populations in Urabá, Antioquia, Colombia represents a risk factor for increased dengue morbidity and mortality. This study aimed to determine the risk of dengue transmission by estimating the population of Ae. aegypti using entomological indices, density of females per dwelling and inhabitant, and virological surveillance in two municipalities in Colombia. A cross-sectional study was conducted with quarterly entomological surveys in three neighborhoods of Apartadó and Turbo between 2021 and 2022. Aedes indices and vector density per dwelling and per inhabitant were calculated. The Kernel method was used for spatial analysis, and correlations between climatic variables and mosquito density were examined. Virus detection and serotyping in mosquitoes was performed using single-step reverse transcription polymerase chain reaction. The housing, reservoir, and Breteau indices were 48.9%, 29.5%, and 70.2%, respectively. The mean density of Ae. aegypti was 1.47 females / dwelling and 0.51 females / inhabitant. The overall visual analysis showed several critical points in the neighborhoods studied. There was significant correlation of vector density and relative humidity and precipitation in the neighborhoods 29 de noviembre and 24 de diciembre. Additionally, serotypes DENV-1 and DENV-2 were found. The overall indices for dwellings, reservoirs, and Breteau were lower than those recorded in 2014 in Urabá. The vector density results in this study were similar to those reported in other studies conducted in Latin America, and vector infection was detected. The Aedes and density indices are complementary, emphasizing the importance of continuous surveillance of Ae. aegypti to inform appropriate control strategies and prevent future dengue outbreaks in these municipalities.

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Section: Discussionsupporting

confidence: 92%

Density of Aedes aegypti and dengue virus transmission risk in two municipalities of Northwestern Antioquia, Colombia

Gómez-Vargas,

Ríos-Tapias,

Marin-Velásquez

et al. 2024

PLoS ONE

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“…Maternally transmitted microorganisms create a core microbiota associated with each species regardless of their geographic distribution (Yadav et al, 2015 ; Mancini et al, 2018 ; Scolari et al, 2021 ; Olmo et al, 2023 ). Wolbachia bacteria, Microsporium fungi, insect-specific viruses, and arboviruses are vertically transmitted microorganisms (Werren, 1997 ; Duguma et al, 2015 ; Velandia-Romero et al, 2017 ; Dickson et al, 2018 ; Scolari et al, 2019 ; Sicard et al, 2019 ).…”

Section: Mosquitoes and Arbovirusmentioning

confidence: 99%

“…The specificity of ISV to invertebrate hosts is related to genetic, structural, immunological, and microclimatic conditions (Elrefaey et al, 2020 ). In addition, multiple infections with arboviruses such as DENV, ZIKV, YFV, and CHIKV have been found in field-collected mosquitoes, with evidence of vertical transmission (Velandia-Romero et al, 2017 ; Alencar et al, 2021 ; Mantilla-Granados et al, 2022 ). Thus, arboviruses are a part of the normal microbiota of these insect vectors, making them reservoirs.…”

Section: Mosquitoes and Arbovirusmentioning

confidence: 99%

A tangled threesome: understanding arbovirus infection in Aedes spp. and the effect of the mosquito microbiota

Mantilla-Granados,

Castellanos,

Velandia-Romero

2024

Front. Microbiol.

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Arboviral infections transmitted by Aedes spp. mosquitoes are a major threat to human health, particularly in tropical regions but are expanding to temperate regions. The ability of Aedes aegypti and Aedes albopictus to transmit multiple arboviruses involves a complex relationship between mosquitoes and the virus, with recent discoveries shedding light on it. Furthermore, this relationship is not solely between mosquitoes and arboviruses, but also involves the mosquito microbiome. Here, we aimed to construct a comprehensive review of the latest information about the arbovirus infection process in A. aegypti and A. albopictus, the source of mosquito microbiota, and its interaction with the arbovirus infection process, in terms of its implications for vectorial competence. First, we summarized studies showing a new mechanism for arbovirus infection at the cellular level, recently described innate immunological pathways, and the mechanism of adaptive response in mosquitoes. Second, we addressed the general sources of the Aedes mosquito microbiota (bacteria, fungi, and viruses) during their life cycle, and the geographical reports of the most common microbiota in adults mosquitoes. How the microbiota interacts directly or indirectly with arbovirus transmission, thereby modifying vectorial competence. We highlight the complexity of this tripartite relationship, influenced by intrinsic and extrinsic conditions at different geographical scales, with many gaps to fill and promising directions for developing strategies to control arbovirus transmission and to gain a better understanding of vectorial competence. The interactions between mosquitoes, arboviruses and their associated microbiota are yet to be investigated in depth.

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“…aegypti larvae and pupae during a dengue outbreak. However, the total specimens, pools positive, pools tested and infection were not specified [ 46 ]. Likewise, using RT-PCR, DENV-2 was recorded in 2 pools of reared Ae.…”

Section: Introductionmentioning

confidence: 99%

Natural vertical transmission of dengue virus in Latin America and the Caribbean: highlighting its detection limitations and potential significance

Golding,

Noble,

Khouri

et al. 2023

Parasites Vectors

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Dengue continues to be a major public health concern in Latin America and the Caribbean with many countries in the region having experienced drastic increases in the incidence of dengue over the past few years. Dengue virus is predominantly transmitted by the bite of an infected female Aedes aegypti mosquito via a process called horizontal transmission. However, the virus may also be transmitted from an infected female mosquito to her offspring by vertical transmission, which occurs via viral invasion of the ovary either at the time of fertilization or during oviposition. In this way, mosquitoes may become dengue virus infected before ever encountering a human host. While some researchers have reported this phenomenon and suggested it may serve as a reservoir for the dengue virus in nature, others have questioned its epidemiological significance because of the low frequency at which it has been observed. Several researchers have either altogether failed to detect it or observed its occurrence at low frequencies. However, some studies have attributed these failures to small sample sizes as well as poor sensitivities of screening methods employed. Therefore, an overview of the occurrence, significance and limitations of detection of vertical transmission of dengue virus in Aedes mosquitoes in nature within Latin America and the Caribbean will be the focus of this review. Graphical Abstract

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Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

Cited by 13 publications

References 20 publications

Density of Aedes aegypti and dengue virus transmission risk in two municipalities of Northwestern Antioquia, Colombia

Density of Aedes aegypti and dengue virus transmission risk in two municipalities of Northwestern Antioquia, Colombia

A tangled threesome: understanding arbovirus infection in Aedes spp. and the effect of the mosquito microbiota

Natural vertical transmission of dengue virus in Latin America and the Caribbean: highlighting its detection limitations and potential significance

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