Dengue virus modulates mitochondrial dynamics during its replication

Abstract

Research over the past few years showed that mitochondrial morphology is a key player in regulation of vital cell physiological processes right from respiration to regulation of apoptosis. Recently the role of mitochondria in virus morphogenesis is gaining importance. In this study, the interaction of dengue virus (DENV) with mitochondria during its replication was investigated, with special emphasis on the association of specific viral proteins with mitochondria. By live cell imaging, it was seen that within an hour of infection, mitochondria showed rapid fission and fusion, indicative of high energy state in the cells. This was followed by recruitment of mitochondria on microtubules to perinuclear region and colocalization with viral factories. As infection progressed, there was circularization and disintegration of mitochondria. In conclusion, our observations suggest a basic dichotomy in the functioning of mitochondria in replication of DENV. Initially, during replication, mitochondria provide energy to ongoing virus replication; while at later stages cells are characterized by mitochondrial dysfunction leading to apoptosis. Of special interest is the implication of viral core protein in induction of apoptosis.The present study is supported by CSIR (F.NO. 9/698(9)/2004‐EMR‐I) and ICMR, India.