Dengue Virus Type 2 Isolated From an Imported Dengue Patient in Japan: First Isolation of Dengue Virus From Nepal

Takasaki, Tomohiko; Kotaki, Akira; Nishimura, Kiyomi; Sato, Yu; Tokuda, Atsuko; Lim, Chang‐Kweng; Ito, Makoto; Tajima, Shigeru; Nerome, Reiko; Kurane, Ichiro

doi:10.1111/j.1708-8305.2007.00165.x

Cited by 45 publications

(38 citation statements)

References 7 publications

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“…It is an endemic nation for many vector borne diseases, like malaria, kala-azar, Japanese encephalitis and lymphatic filariasis (Dumre et al, 2013). The first report of dengue fever came in 2004 from a Japanese traveler in Nepal and it was reported to be of serotype 2 (Takasaki et al, 2008) with >99% similarity with viral strains from India. It was followed by a native report in 2006 with detection of four dengue serotypes (Malla et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic study reveals co-circulation of Asian II and Cosmopolitan genotypes of Dengue virus serotype 2 in Nepal during 2013

Singh

Gupta

Manakkadan

et al. 2015

Infection, Genetics and Evolution

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Section: Introductionmentioning

confidence: 99%

Phylogenetic study reveals co-circulation of Asian II and Cosmopolitan genotypes of Dengue virus serotype 2 in Nepal during 2013

Singh

Gupta

Manakkadan

et al. 2015

Infection, Genetics and Evolution

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“…The isolated DENV-2 (GenBank accession no. AB194882) was 98% homologous with DENV-2 isolated in India (8). The prevalence of dengue virus antibody was reported to be 10.4% in the southwestern region of Nepal (9).…”

Section: Dengue Virus Nepalmentioning

confidence: 99%

Dengue Virus, Nepal

Pandey

Morita

Khanal

et al. 2008

Emerg. Infect. Dis.

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“…6 DF is the emerging disease affecting Nepal since 2004 although DF has been already observed in foreign visitor earlier. 7,8 Nepal reported indigenous transmission of dengue cases in November 2006. 9 The outbreak occurred in Nepal following the Indian epidemic of DF/DHF in September-October 2006.…”

Section: Introductionmentioning

confidence: 99%

Sero-diagnosis of Dengue virus in Different Hospitals of Nepal

Shah¹,

Khadka

et al. 2013

Int J Infection & Microbiol

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INTRODUCTION: Dengue fever (DF) is an emerging mosquito borne viral disease and important public health problem in low land Terai region which is also moving towards hilly region Nepal. This study was designed to determine the sero-prevalence of dengue virus infection in patients visiting hospitals of Nepal. MATERIALS AND METHODS: This study was conducted during period (June-November) of 2010 in Nepalese patients with fever visiting hospitals of Birganj, Damouli, Biratanagar, Dhading Besi and Chitwan. The sero-prevalence of dengue virus specific IgM was determined by enzyme linked immunosorbent assay (ELISA). Serum samples were collected from 289 patients visiting hospitals with history of fever and clinically suspected dengue fever. RESULTS: The anti-dengue IgM positivity was found to be 8.99%. The positive dengue cases were higher in male (10.8%) as compared to female (7.1%) though it was not statistically significant (P>0.05). Among different age groups, the highest positive cases (12.3%) were from age group below 15 years followed by above 50 years 8.3%. Out of 5 hospitals, the highest positive cases were in Tanahu hospital, Damouli (23.8%) followed by Bharatpur hospital and Chitwan (22.2%). Age and gender were found to be independent predictors. The highest numbers of dengue positive cases were in occupation group business (13.3%) followed by agriculture (12.7%). CONCLUSIONS: Prevalence of dengue virus infection is increasing and proper control measure should be provided. IgM capture ELISA was used for laboratory analysis and remains as a reliable and inexpensive method for the diagnosis of dengue. Hence, the IgM capture ELISA has become the most accepted technique for the diagnosis of dengue in developing countries like Nepal. DOI: http://dx.doi.org/10.3126/ijim.v1i2.7003 Int J Infect Microbiol 2012;1(1):58-62

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Dengue Virus Type 2 Isolated From an Imported Dengue Patient in Japan: First Isolation of Dengue Virus From Nepal

Abstract: We report the isolation of dengue virus type 2 from a dengue patient returning to Japan from Nepal in October, 2004. This is the first isolate of dengue virus in Nepal. According to nucleotide homology, the virus was closest to a dengue virus type 2 isolate from India.

Cited by 45 publications

References 7 publications

Phylogenetic study reveals co-circulation of Asian II and Cosmopolitan genotypes of Dengue virus serotype 2 in Nepal during 2013

Phylogenetic study reveals co-circulation of Asian II and Cosmopolitan genotypes of Dengue virus serotype 2 in Nepal during 2013

Dengue Virus, Nepal

Sero-diagnosis of Dengue virus in Different Hospitals of Nepal

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