Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia

Brugnon, Florence; Ouchchane, Lemlih; Pons-Rejraji, Hanae; Artonne, Christine; Farigoule, M.; Janny, L.

doi:10.1093/humrep/det253

Cited by 49 publications

(45 citation statements)

References 74 publications

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“…The current findings relating to sperm motility improvement are in agreement with the results performed on ram [41], buffalo [42], goat [43], and fish [44]. However, there is also evidence that HT and/or T do not have an impact on sperm motility, as was observed in human [17, 24], dog [45], bull [46], and Boer goat [31]. Such discrepancies mostly depend on the species used in the investigations.…”

Section: Discussionsupporting

confidence: 90%

The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

Partyka

Rodak

Bajzert

et al. 2017

BioMed Research International

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The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen. Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation. Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry. The highest sperm motility and the highest percentage of viable sperm were in the HT1 group (P < 0.01 and P < 0.05) following cryopreservation. After thawing, we observed a higher percentage of sperm without apoptosis and membrane reorganization changes in the LC1 and T1 group when compared to the control (P < 0.05). There was a higher percentage of live sperm without lipid peroxidation (LPO) in all treatments (P < 0.01; P < 0.05), when compared to the control group. The percentage of sperm with high mitochondrial potential significantly increased with LC1, T1, and T10 (P < 0.05). Supplementation of the diluent with LC1, LC5, and T1 significantly (P < 0.05) reduced DNA susceptibility to fragmentation, compared to the control and HT1 groups. These results indicate that the addition of examined antioxidants improves the quality of cryopreserved chicken semen.

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Section: Discussionsupporting

confidence: 90%

The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

Partyka

Rodak

Bajzert

et al. 2017

BioMed Research International

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“…The explanation for this finding may relate to sperm washing selection of the sperm with good motility (e.g., resistant to cryoinjury) and elimination of the other components in semen (i.e., ROS, leukocytes, bacteria, etc.) that affect the post-thawing sperm outcome [12,[16][17][18][19]30]. The percentages of sperm motility in the static, slow velocity, and medium velocity subgroups of the SAFT group were higher than in the SBF group.…”

Section: Discussionmentioning

confidence: 98%

“…Although pre-frozen sperm quality, especially sperm preparation prior to freezing, has been studies [18,22,28], the effects of sperm preparation before freezing on the postthawing sperm quality, as part of optimizing sperm recovery, especially with regards to apoptosis, have not been established yet. Previous studies were only focused on the effect of cryopreservation in sperm when comparing fresh sperm and post-cryopreserved sperm [18,22]. The advantages of prefrozen sperm preparation have been demonstrated in the previous studies, but the relevant experiments were designed to compare sperm preparation and a "no treatment" control group [28,29].…”

Section: Discussionmentioning

confidence: 99%

“…Although semen analysis indicated normal results, a number of abnormal spermatozoa or leukocytes could be identified in the sample. Those cells generated reactive oxygen species (ROS), which can penetrate through the sperm plasma membrane and initiate intrinsic ROS formation inside the spermatozoa, causing sperm damage, e.g., DNA fragmentation [12,[14][15][16][17][18][19]. After cryopreservation of spermatozoa, the percentage of abnormal spermatozoa in regards to motility, morphology, and DNA integrity significantly increases after freezing-thawing process [2,19,20].…”

Section: Introductionmentioning

confidence: 99%

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Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Petyim

Neungton

Thanaboonyawat

et al. 2014

J Assist Reprod Genet

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Purpose To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation. Methods Semen samples from 65 infertile males were equally divided into two aliquots one of which was processed for swim-up prior to cryopreservation and one of which was processed following cryopreservation. Sperm count, motility, and apoptosis index were measured in each group. Result (s) The total sperm count and the total motile sperm count decreased after thawing in both the pre-preparation and non-preparation groups compared with neat semen group (P<0.001). Moreover, the percentage of apoptotic sperm in the pre-preparation group after cryopreservation was lower than that in the non-preparation group (P<0.05), whereas the percentage of vital sperm with progressive motility was higher than that in the pre-preparation group (P<0.001). Conclusion (s) Semen preparation by swim-up before freezing resulted in better sperm quality and fewer apoptotic sperm than sperm preparation after thawing. Therefore, sperm preparation before cryopreservation should be considered in routine sperm cryopreservation.

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“…However, seminal plasma has also been shown to contain cells such as aging sperms, leukocytes and epithelial cells that can generate reactive oxygen species and can lead to oxidative and apoptotic injury during the freezing and thawing procedure [14]. Another study reported that sperm selection by density gradient centrifugation before freezing allows the selection of a better functional sperm population with more resistance to the cryopreservation processes [29]. In the present study, we used spermatozoa obtained by density gradient centrifugation and washing to eliminate the leukocytes and epithelial cells that might produce oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men

Turan¹,

Şentürk²,

Ercan³

2017

Marmara Medical Journal

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Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men Kriyopreservasyon oligoastenoteratozoospermik erkeklerde DNA fragmantasyonunu ve ultrastrüktürel hasarı tetikler ÖZ Amaç: Oligoastenoteratozoospermi (OAT) erkek infertilitisi sebeplerinden biridir. Kriyopreservasyon infertilite yönetimi için değerlidir. Bu çalışmanın amacı, OAT'lı hastalarda sperm motilitesi, morfolojisi, ultrastrüktürel detaylar ve DNA fragmentasyonu üzerine kriyopreservasyonun etkilerini ortaya çıkarmaktır. Gereç ve Yöntem: Bu gözlemsel çalışmada, gönüllü 20 normospermik ve 20 OAT'lı hastadan ejakulatlar toplanmıştır. Ejakulatlar, -196 o C sıvı nitrojende saklanmış ve çözdürmeden üç ve altı ay sonra analiz edilmiştir. Ejakulatlarda, motilite, morfoloji ve DNA fragmantasyonu dondurma öncesi ve sonrasında değerlendirilmiştir. Bulgular: Her iki grupta da sperm motilitesi ve morfolojisi kriyopresvasyondan etkilenmiştir. Her iki grupta da çözdürmeden sonra morfolojik değişiklikler anlamlı artmıştır. Ultrastrüktürel incelemeler membran bütünlüğünün değiştiğini ve akrozomal hasarın arttığını göstermiştir. Her iki grupta da, dondurma öncesi ile karşılaştırıldığında, çözdürme sonrasında DNA kırıkları olan hücrelerin arttığı gözlenmiştir. Her iki dondurma-çözme periyodunda, kontrol grubu ile kıyaslandığında OAT grubunda DNA kırıkları olan spermatozoa sayısının anlamlı olarak daha yüksek olduğu gözlenmiştir. Sonuç: Kriyopreservasyon, hem normospermik hem de OAT gruplarında ultrastrüktürel hasar yapmaktadır ve DNA hasarını indüklemektedir. Bu hasar OAT hastalarında daha ciddidir.

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Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia

Abstract: This work was supported by a hospital grant (Projet Hospitalier Recherche Clinique, CHU Clermont Ferrand, France). None of the authors has any conflict of interest to declare.

Cited by 49 publications

References 74 publications

The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men

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