Density gradient characterization of disrupted and untreated eastern encephalitis virus

The mode of assembly of alphavirus cores implies a mechanism for the disassembly of the cores in the early stages of infection

“…In the context of this review some important results are briefly summarized below and much of the data is discussed in more detail in the subsequent sections. Alphavirus cores have a sedimentation value of about, 150 S and in addition to the infectious 42 S genomc RNA they contain about 250 molecules of a single species of protein, the core (C) protein, which has a molecular weight of about 30,000 Dalton (2,3,5,13,24,25,26,29,32,48,54,60). The cores are isometric particles about 40 nm in diameter (14,22,33,43).…”

Monoclonal Antibodies to Sindbis Virus Glycoprotein E1 can Neutralize, Enhance Infectivity, and Independently Inhibit Haemagglutination or Haemolysis

“…Biological functions associated with the surface of the virus include neutralization of infectivity, haemagglutination (Clarke & Casals, 1958) and haemolysis (Karabatsos, 1963(Karabatsos, , 1965(Karabatsos, , 1973Goto et al, 1979;V~i~in~inen & K~i~iri~iinen, 1980;Yoshinaka et al, 1978).…”

Hemolytic Activity of a Togavirus, Getah*

“…The hemolytic activity of togavirus has been reported previously by Karabatsos (8)(9)(10), but the fundamental mechanism of hemolysis of chick red blood cells has not yet been studied.…”

“…This report describes studies on the HE activity of a representative togavirus, Getah (GET). Conditions which remove the HE activity but not the hemagglutinating activity are discussed, and the HE reactions of purified GET virus are compared to those previously investigated using crude virus extracts (8)(9)(10). MATERIALS …”

“…Among these are paramyxoviruses such as Sendai (HVJ), mumpus, measles, and Newcastle virus (NDV) (2,6,7,11) and togaviruses (8)(9)(10). Since togaviruses such as Sindbis virus are comparatively simple in their chemical nature, they provide a model for studying the mechanism of the activity.…”