Dental pulp stem cell-derived extracellular matrix: autologous tool boosting bone regeneration

Alksnė, Milda; Kalvaitytė, Miglė; Šimoliūnas, Egidijus; Gendvilienė, Ieva; Barasa, Povilas; Rinkūnaitė, Ieva; Kaupinis, Algirdas; Šeinin, Dmitrij; Rutkūnas, Vygandas; Bukelskienė, Virginija

doi:10.1016/j.jcyt.2022.02.002

Cited by 17 publications

(12 citation statements)

References 86 publications

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“…The essential steps are providing a substrate for cells to grow, followed by providing the necessary nutrients and physiological conditions, and the the cells will produce the ECM that can be decellularized and used. This can achieve simple sheets of dECM that can be used for other experiments or procedures [ 101 , 103 , 126 , 209 , 247 , 248 , 249 , 250 , 251 , 252 , 253 , 254 , 255 , 256 , 257 ], the modification of existing dECM to include tissue-specific ECM [ 258 ], or the modification of the surface of synthetic substrates [ 84 , 204 , 205 , 259 , 260 , 261 ]. Such a technique can be used in more complex situations, such as the surgical implantation of a scaffold for autologous cells to produce the ECM, followed by explanting then decellularizing the resulting scaffold, and finally implanting it in the orthotopic location [ 262 ].…”

Section: Methods Of Preparing Decmmentioning

confidence: 99%

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

McInnes

Möser

Chen

2022

JFB

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The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize the practise of medicine through the abilities to repair, regenerate, or replace tissues and organs with functional engineered constructs. To this end, tissue engineering combines scaffolding materials with cells and biologically active molecules into constructs with the appropriate structures and properties for tissue/organ regeneration, where scaffolding materials and biomolecules are the keys to mimic the native extracellular matrix (ECM). For this, one emerging way is to decellularize the native ECM into the materials suitable for, directly or in combination with other materials, creating functional constructs. Over the past decade, decellularized ECM (or dECM) has greatly facilitated the advance of tissue engineering and regenerative medicine, while being challenged in many ways. This article reviews the recent development of dECM for tissue engineering and regenerative medicine, with a focus on the preparation of dECM along with its influence on cell culture, the modification of dECM for use as a scaffolding material, and the novel techniques and emerging trends in processing dECM into functional constructs. We highlight the success of dECM and constructs in the in vitro, in vivo, and clinical applications and further identify the key issues and challenges involved, along with a discussion of future research directions.

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Section: Methods Of Preparing Decmmentioning

confidence: 99%

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

McInnes

Möser

Chen

2022

JFB

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“…They are more accessible than BMSCs and have been recognized as a cellular source for regenerative medicine [ 230 ]. Studies have demonstrated positive outcomes with the incorporation of DPSCs in the context of BTE [ 231 ]. However, there are cases where stem cells fail to be recruited or lack adherence to the scaffolds.…”

Section: Biological Factors and Bioactive Moleculesmentioning

confidence: 99%

Three-Dimensional Printing Methods for Bioceramic-Based Scaffold Fabrication for Craniomaxillofacial Bone Tissue Engineering

Sheikh,

Nayak,

Daood

et al. 2024

JFB

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Three-dimensional printing (3DP) technology has revolutionized the field of the use of bioceramics for maxillofacial and periodontal applications, offering unprecedented control over the shape, size, and structure of bioceramic implants. In addition, bioceramics have become attractive materials for these applications due to their biocompatibility, biostability, and favorable mechanical properties. However, despite their advantages, bioceramic implants are still associated with inferior biological performance issues after implantation, such as slow osseointegration, inadequate tissue response, and an increased risk of implant failure. To address these challenges, researchers have been developing strategies to improve the biological performance of 3D-printed bioceramic implants. The purpose of this review is to provide an overview of 3DP techniques and strategies for bioceramic materials designed for bone regeneration. The review also addresses the use and incorporation of active biomolecules in 3D-printed bioceramic constructs to stimulate bone regeneration. By controlling the surface roughness and chemical composition of the implant, the construct can be tailored to promote osseointegration and reduce the risk of adverse tissue reactions. Additionally, growth factors, such as bone morphogenic proteins (rhBMP-2) and pharmacologic agent (dipyridamole), can be incorporated to promote the growth of new bone tissue. Incorporating porosity into bioceramic constructs can improve bone tissue formation and the overall biological response of the implant. As such, employing surface modification, combining with other materials, and incorporating the 3DP workflow can lead to better patient healing outcomes.

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“…Regarding application, DPSCs can be seeded on another material, cultured for some time, and then treated with a decellularized method to form the decellularized composite material. For instance, DPSCs were cultured in a collagen/chitosan hydrogel for 2 weeks or grown on 3D-printed porous polylactic acid scaffolds for 7 days and then were decellularized [ 69 , 70 ]. These dECM-composites had favorable effects, indicating their potential application in pulp regeneration.…”

Section: Strategiesmentioning

confidence: 99%

Creating a Microenvironment to Give Wings to Dental Pulp Regeneration—Bioactive Scaffolds

Jiang

et al. 2023

Pharmaceutics

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Dental pulp and periapical diseases make patients suffer from acute pain and economic loss. Although root canal therapies, as demonstrated through evidence-based medicine, can relieve symptoms and are commonly employed by dentists, it is still difficult to fully restore a dental pulp’s nutrition, sensory, and immune-regulation functions. In recent years, researchers have made significant progress in tissue engineering to regenerate dental pulp in a desired microenvironment. With breakthroughs in regenerative medicine and material science, bioactive scaffolds play a pivotal role in creating a suitable microenvironment for cell survival, proliferation, and differentiation, following dental restoration and regeneration. This article focuses on current challenges and novel perspectives about bioactive scaffolds in creating a microenvironment to promote dental pulp regeneration. We hope our readers will gain a deeper understanding and new inspiration of dental pulp regeneration through our summary.

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Dental pulp stem cell-derived extracellular matrix: autologous tool boosting bone regeneration

Cited by 17 publications

References 86 publications

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

Three-Dimensional Printing Methods for Bioceramic-Based Scaffold Fabrication for Craniomaxillofacial Bone Tissue Engineering

Creating a Microenvironment to Give Wings to Dental Pulp Regeneration—Bioactive Scaffolds

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