Dental Pulp Stem Cell Niche

Yu, Jinhua; Jamal, Mohamed; Garcı́a-Godoy, Franklin; Huang, George T.‐J.

doi:10.1007/978-3-319-21705-5_8

Cited by 4 publications

(3 citation statements)

References 128 publications

(146 reference statements)

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“…These factors act synergistically or antagonistically and as a network to regulate the status of DPSCs in the niches. Notably a complex network of biochemical signaling pathways including Notch and WNT/β-Catenin signaling, many growth factors such as vascular endothelial growth factor (VEGF), transforming growth factor (TGF)-β and ECM proteins participate in maintaining and regulating homeostasis in the DPSC niche as well as in the regulation of the proliferation and differentiation of DPSCs ( Mitsiadis et al, 2007 ; Yu et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Dental Pulp Stem Cell Mechanoresponsiveness: Effects of Mechanical Stimuli on Dental Pulp Stem Cell Behavior

et al. 2018

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Dental pulp is known to be an accessible and important source of multipotent mesenchymal progenitor cells termed dental pulp stem cells (DPSCs). DPSCs can differentiate into odontoblast-like cells and maintain pulp homeostasis by the formation of new dentin which protects the underlying pulp. DPSCs similar to other mesenchymal stem cells (MSCs) reside in a niche, a complex microenvironment consisting of an extracellular matrix, other local cell types and biochemical stimuli that influence the decision between stem cell (SC) self-renewal and differentiation. In addition to biochemical factors, mechanical factors are increasingly recognized as key regulators in DPSC behavior and function. Thus, microenvironments can significantly influence the role and differentiation of DPSCs through a combination of factors which are biochemical, biomechanical and biophysical in nature. Under in vitro conditions, it has been shown that DPSCs are sensitive to different types of force, such as uniaxial mechanical stretch, cyclic tensile strain, pulsating fluid flow, low-intensity pulsed ultrasound as well as being responsive to biomechanical cues presented in the form of micro- and nano-scale surface topographies. To understand how DPSCs sense and respond to the mechanics of their microenvironments, it is essential to determine how these cells convert mechanical and physical stimuli into function, including lineage specification. This review therefore covers some aspects of DPSC mechanoresponsivity with an emphasis on the factors that influence their behavior. An in-depth understanding of the physical environment that influence DPSC fate is necessary to improve the outcome of their therapeutic application for tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Dental Pulp Stem Cell Mechanoresponsiveness: Effects of Mechanical Stimuli on Dental Pulp Stem Cell Behavior

et al. 2018

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“…Extracellular proteins and growth factors induce stem cells differentiation depending on their niche biology. [ 4 6 ] Hyaluronic acid (HA) is a glycosaminoglycan and the main component of the extracellular matrix that can create an adequate microenvironment, accelerate cell proliferation and improve healing which is important in tissue regeneration. [ 7 8 ]…”

Section: Introductionmentioning

confidence: 99%

The effect of hyaluronic acid conditioned media on hDPSCs differentiation through CD44 and transforming growth factor-β1 expressions

Bagio

Lestari²,

Putra³

et al. 2023

Journal of Advanced Pharmaceutical Technology &Amp; Research

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Hyaluronic acid (HA) has the capability to influence dentin niche which is important in regenerative process. The CD44 as a specific receptor of HA was found to be related to dentin mineralization process. Meanwhile, transforming growth factor β1 (TGF-β1) has a vital role in the transition from proliferation into the differentiation of human dental pulp stem cell human dental pulp stem cells (hDPSCs) to become odontoblast cells and dentin mineralization. This study aims to analyzed HA's effect on dentin mineralization through CD44 and TGF-β1 expressions. Stem cells were cultured in four different supplemented conditioned media (control, +10 μg/mL, +20 μg/mL, and + 30 μg/mL of HA). Evaluation of CD44 expression was analyzed using flow cytometry and TGF-β1 was analyzed using enzyme-linked immunosorbent assay reader. Qualitative result using Alizarin red test after 21 days was done to confirm the formation of mineralization nodules. It was shown that HA expression of CD44 and TGF-β1 on hDPSCs were higher in AH groups compared to the control group and 30 μg/mL HA induced the highest TGF-β1 expression on hDPSCs. Alizarin red test also showed the highest mineralization nodules in the same group. Therefore, from this study, we found that supplemented 30 μg/mL of HA was proved in initiating hDPSCs differentiation process and promote dentin mineralization.

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“…Hyaluronic acid (HA) is a glycosaminoglycan and an important component of the extracellular matrix in the dentine niche ( 14 ). Extracellular matrix proteins play an important role in regulating stem cell homeostasis and differentiation in the microbiological environment of the dentine niche ( 15 ). Hyaluronic acid, in various molecular weights, are known to have an important role in each stage of regeneration including the repair of the extracellular matrix, the process of migration, proliferation and differentiation of cells ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Hydrogel Hyaluronic Acid on Dentine Sialophosphoprotein Expression of Human Dental Pulp Stem Cells

NUGRAHENI

2023

Eur Endod J

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Objective Hyaluronic acid (HA) is glycosaminoglycan and one of important factors in extracellular matrix. In an inflamed pulp, when niche biology is conducive, the recruitment of human dental pulp stem cells (hDPSCs) will take place and differentiate into odontoblast like cell, creating reparative dentine and expressing dentine sialophosphoprotein (DSPP). Therefore, the purpose of this study was to analyze the potential of hydrogel HA in various concentration towards hDPSCs differentiation via DSPP expression at day 7 and 14. Methods After hDPSCs incubation reaching 80% confluence, cells were then starved for 24 hours. Then, culture media were supplemented with osteogenic media. hDPSCs planted into 96 well plate and HA 10 µg/mL, 20 µg/mL, and 30 µg/mL were added. DSPP expression was analysed using elisa reader at day 7 and 14, qualitative result was analysed using alizarin red at day 21. Data was analysed using one-way ANOVA. Results At day 7, there was a statistically significant different potential of HA conditioned media in various concentration (p<0.05) towards hDPSCs differentiation via expression of DSPP with HA 30 µg/mL being the most potential concentration to increase DSPP expression. Conclusion HA have the potential to increase odontoblast differentiation process via expression of DSPP, with HA 30 µg/mL being the optimum concentration for hDPSCs.

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Dental Pulp Stem Cell Niche

Cited by 4 publications

References 128 publications

Dental Pulp Stem Cell Mechanoresponsiveness: Effects of Mechanical Stimuli on Dental Pulp Stem Cell Behavior

Dental Pulp Stem Cell Mechanoresponsiveness: Effects of Mechanical Stimuli on Dental Pulp Stem Cell Behavior

The effect of hyaluronic acid conditioned media on hDPSCs differentiation through CD44 and transforming growth factor-β1 expressions

The Effect of Hydrogel Hyaluronic Acid on Dentine Sialophosphoprotein Expression of Human Dental Pulp Stem Cells

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