Dental pulp stem cells‐based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo

Abstract

Objectives: As a result of the current limitation of therapeutic strategies, the repair and regeneration of oviduct injuries required an alternative treatment. We present a novel approach to treat oviduct injuries through a dental pulp stem cells (DPSCs)based therapy.Materials and Methods: In vitro and in vivo models have been established. Immunofluorescence staining, flow cytometry and enzyme-linked immunosorbent assay (ELISA) analysis were used to investigate the features and angiogenic properties of DPSCs, as well as their impact on macrophages, in vitro. For the in vivo experiment with female SD rat model, immunohistochemical staining and ELISA analysis were used to assess the effects of DPSCs on the repair and regeneration of damaged oviducts. Results:The present data showed that intraperitoneal injection of DPSCs reduced the expression of IL-6 and TNF-α to inhibit the immunoreaction in injured sites, as well as increased the expression of VEGF to promote the in situ formation of vessellike structures, thus the repair and recovery process could be initiated. Conclusions:We concluded that DPSCs-based therapy could be a novel potential technique for restoring the structure and function of damaged oviduct by enhancing immuno-regulated effect and promoting angiogenic property.

Inhibitory potential of nanoscale dental pulp stem cell lysate in lung cancer with risk of lymph node metastasis

“…There are some reports suggested that IL-1β blockade may be a preventive strategy for high risk individuals [44]. In our previous study, we found that both DPSC and DPSC lysate could signi cantly inhibit the expression of IL-1β [29,45]. This may be one of the mechanisms by which DPSC lysate inhibits tumor.…”

Inhibitory effects of the nanoscale lysate derived from xenogenic dental pulp stem cells in lung cancer models

“…The anti-proliferative and pro-apoptotic effects of EVSs derived from UMSC were detected in bladder carcinoma [ 45 ].There are some reports suggested that IL-1β blockade may be a preventive strategy for high risk individuals [ 46 ]. In our previous study, we found that both DPSC and DPSC lysate could significantly inhibit the expression of IL-1β [ 19 , 47 ]. This may be one of the mechanisms by which DPSC lysate inhibits tumor.…”

Photosensitive Hydrogels Encapsulating DPSCs and AgNPs for Dental Pulp Regeneration

“…Dental pulp stem cells (DPSCs) possess mesenchymal stem cell–like properties and have advantages such as source-abundant, readily available, ethically uncontroversial, and low-immunogenicity characteristics. 6 , 7 , 8 As for experiments in dental pulp regeneration in vivo, some studies have shown that DPSCs were able to differentiate into odontoblastlike and endotheliumlike cells to reconstruct the structure of the pulp-dentin complex. 9 , 10 , 11 In addition, DPSCs had the ability to proliferate rapidly and maintain a good stemness, even if the culture medium without the addition of animal serum or exogenous growth factors in vitro.…”