Dental pulp stem cells in endodontic research:  a promising tool for tooth tissue engineering

Gandhi, Amit; Gandhi, Taru; Madan, Natasha

doi:10.21726/rsbo.v8i3.1080

Cited by 5 publications

(3 citation statements)

References 35 publications

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“…This cell can self-renew by splitting into multiple lineages. Because third molar roots are often absent by the age of 18, these teeth have a large pool of undifferentiated cells in the dental germ pulp (cell rich zone) [ 28 , 29 ]. Odontoblasts, apical vasculature, and periodontal ligament are mesenchymal components of teeth [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Pulp Tissue Viability and Cytotoxicity of Pulp Capping Agents

Panda

Govind

Sahoo

et al. 2023

JCM

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The present research study assessed the cell viability and cytotoxic effect of mineral tri-oxide aggregate (MTA), Tetric N-Bond Universal bonding agent, Theracal PT (pulpotomy treatment), and platelet-rich fibrin (PRF) as pulp capping agents on human dental pulp stem cells (hDPSCs). The cells were isolated from the pulp tissue of an extracted healthy permanent third molar. After four passages in Dulbecco’s Modified Eagle’s Medium, the primary cells were employed for the investigation. The test materials and untreated cells (negative control) were subjected to an Methylthiazol-diphenyl-tetrazolium (MTT) cytotoxicity assay and assessed at 24-, 48-, and 72-h intervals. The Wilcoxon matched-paired t-test and Kruskal–Wallis analysis of variance (ANOVA) test were applied (p < 0.05). PRF imparted the highest cell viability at 48 h (p < 0.001), followed by MTA, Theracal PT, and Tetric N-Bond. Similarly, PRF had the highest potential to enhance cell proliferation and differentiation (p < 0.001), followed by Theracal PT, MTA, and the bonding agent at the end of 24 h and 72 h, respectively. Finally, PRF sustained the viability of human primary dental pulp stem cells more effectively than Theracal PT and MTA; however, the application of a Tetric N-Bond as a pulp capping agent was ineffective.

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Section: Discussionmentioning

confidence: 99%

Analysis of Pulp Tissue Viability and Cytotoxicity of Pulp Capping Agents

Panda

Govind

Sahoo

et al. 2023

JCM

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“…In-vitro harvesting, growing, and differentiation of cells and seeding cells onto scaffolds for their invivo implantation has been a common technique followed for regeneration. There have also been times when the scaffolds have been once again re-implanted onto the extracted tooth socket or the jaw [26].…”

Section: Whole Tooth Regenerationmentioning

confidence: 99%

Application of Stem Cells in Endodontics

Vishwanathaiah¹

2023

TIJPH

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The discovery of stem cells in dental pulp has expanded our understanding concerning the various mechanisms that play a substantial role in the maintenance of dental pulp homeostasis to improve health and also regarding the pulp response to injury. Stems cells play a pivotal role in the physiology of the dental pulp tissue throughout the entire lifespan of the tooth. The ideology that these cells are intimately associated with regulating pulp angiogenesis as a response to cariogenic challenges is also inferred. In recent times, we are witnessing an increasing number of passionate individuals showing enthusiasm in the field of regenerative endodontics as stem cells exhibit potential benefits in dental pulp tissue engineering. The article here focuses predominantly on stem cells—the dental stem cells and non-dental stem cells which play an active part in benefitting pulpal necrosis tooth, the cardinal objective of these cells in the field of endodontic therapy, and the current, as well as the future role, stem cells could play in the clinical practice of endodontics eventually. Keywords: Dental stem cells, Pulpal necrosis, Stem cells, Regenerative.

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“…These stem cells can differentiate into odontoblasts in the presence of the required growth factors [ 6 ]. Tissue engineering requires three major components of a scaffold, morphogen growth factor (s), and mesenchymal stem cells for the regeneration of functional tissue or organ [ 4 , 7 ]. Apical papilla is a tissue that is located near the apex of the roots of immature teeth, acting as a source of stem cells that differentiate into the dentin-synthesizing odontoblasts, which play a significant role in root formation and regenerative endodontic treatments [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of copper oxide nanoparticles and light-emitting diode irradiation on the cell viability and osteogenic/odontogenic differentiation of human stem cells from the apical papilla

Karkehabadi¹,

Rahmati²,

Abbasi³

et al. 2023

BMC Oral Health

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Objectives This experimental study aimed to assess the effect of copper oxide nanoparticles (CuONPs) and light-emitting diode (LED) irradiation on the cell viability and osteogenic/odontogenic differentiation of human SCAPs. Methods After the culture of SCAPs, the effects of different concentrations of CuONPs on cell viability were evaluated by the methyl thiazolyl tetrazolium (MTT) assay after 24 and 48 h, and the optimal concentration was determined (n = 12). SCAPs were then divided into four groups based on the type of treatment: (I) no-treatment control group, (II) exposure to CuONPs, (III) LED irradiation (635 nm, 200 mW/cm2) for 30 s, and (IV) exposure to CuONPs combined with LED irradiation. CuONPs were synthesized by a green technique, which was based on reduction and simultaneous stability of copper ions by using the pomegranate peel extract. After treatments, the expression of osteogenic/odontogenic markers including dentin sialophosphoprotein (DSPP), bone sialoprotein (BSP), alkaline phosphatase (ALP), and dentin matrix acidic phosphoprotein 1 (DMP1) was evaluated in all four groups using quantitative real-time polymerase chain reaction (PCR) (n = 16). Also, osteogenic differentiation of SCAPs was evaluated qualitatively by alizarin red staining (ARS) to assess the matrix mineralization (n = 4). SPSS version 18 was used for data evaluation. The Kruskal–Wallis and Mann–Whitney tests were used to compare the groups. Results Exposure to 1 µg/mL CuONPs resulted in maximum viability of SCAPs. Concentrations of CuONPs over 10 µg/mL significantly decreased the viability of SCAPs. Real-time PCR showed that the expression of DMP1, BSP, ALP, and DSPP in CuONPs + LED and LED groups was significantly higher than that in CuONPs and control groups at both 24 and 48 h (P < 0.05). The density of ARS increased in all experimental groups after 24 h, and in CuONPs + LED and CuONPs groups after 48 h, compared to the control group. Conclusion Addition of CuONPs and LED irradiation of SCAPs in the culture medium significantly enhanced their osteogenic/odontogenic differentiation.

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Dental pulp stem cells in endodontic research: a promising tool for tooth tissue engineering

Cited by 5 publications

References 35 publications

Analysis of Pulp Tissue Viability and Cytotoxicity of Pulp Capping Agents

Analysis of Pulp Tissue Viability and Cytotoxicity of Pulp Capping Agents

Application of Stem Cells in Endodontics

Effect of copper oxide nanoparticles and light-emitting diode irradiation on the cell viability and osteogenic/odontogenic differentiation of human stem cells from the apical papilla

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