Dental pulp stem cells: State of the art and suggestions for a true translation of research into therapy

Noce, Marcella La; Paino, Francesca; Spina, Anna; Naddeo, Pasqualina; Montella, Roberta; Desiderio, Vincenzo; Rosa, Alfredo De; Papaccio, Gianpaolo; Tirino, Virginia; Laino, Luigi

doi:10.1016/j.jdent.2014.02.018

Cited by 160 publications

(132 citation statements)

References 72 publications

Supporting

Mentioning

127

Contrasting

Unclassified

Order By: Relevance

“…Microfluidics for analysing stem cell niches area (Giuliani et al, 2013;Huang et al, 2009;Leucht et al, 2008;La Noce et al, 2014). It has been shown that the embryonic origin of stem cells, combined with their Hox expression profile, play a key role in determining their fate during bone regeneration (Leucht et al, 2008).…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

“…Therefore, stem cells guarantee tissue repair and regeneration throughout life. During the last decades, a plethora of adult stem cell populations have been isolated from different locations of orofacial organs, characterised, and tested for their potential applications in regenerative medicine (Mao et al, 2012;Mitsiadis et al, 2012;La Noce et al, 2014). Adult stem cells could be removed from a patient, expanded and placed back into the same individual when tissue repair becomes necessary, thereby removing the need for immunosuppression Mitsiadis et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…However, BMSCs cells do not have the same embryonic origin as the cells forming the orofacial tissues. Moreover, numerous studies reported that BMSCs differ from MSCs isolated from the orofacial 216 www.ecmjournal.org P Pagella et alMicrofluidics for analysing stem cell niches area (Giuliani et al, 2013;Huang et al, 2009;Leucht et al, 2008;La Noce et al, 2014). It has been shown that the embryonic origin of stem cells, combined with their Hox expression profile, play a key role in determining their fate during bone regeneration (Leucht et al, 2008).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Pagella

Neto

Lamghari

et al. 2015

eCM

View full text Add to dashboard Cite

Stem cell-based mediated therapies represent very promising approaches for tissue regeneration and are already applied with success in clinics. These therapeutic approaches consist of the in vitro manipulation of stem cells and their consequent administration to patients as living and dynamic biological agents. Nevertheless, the deregulation of stem cells function might result in the generation of pathologies such as tumours or accelerated senescence. Moreover, different stem cells sources are needed for regeneration of specific tissues. It is thus fundamental to understand the mechanisms regulating the physiology of stem cells. Microfluidic technology can be used to mimic in vivo scenarios and allow the study of stem cell physiology at both single cell and whole stem cell niche levels. This review focuses on the potential sources of stem and progenitor cells for orofacial regeneration and the use of microfluidic technologies for the study of stem cells behaviour and stem cell niches, in the light of regenerative medicine. Keywords:Microfluidics, stem cells, stem cell niches, orofacial regeneration, tooth, innervation, trigeminal ganglia, regenerative medicine. IntroductionThe development of organs and tissues that belong to the orofacial complex proceeds through a series of inductive interactions between cells originated from the epithelium, mesoderm and cranial neural crest-derived mesenchyme (Mao et al., 2012;Mitsiadis and Graf, 2009;Mitsiadis and Papagerakis, 2011). Orofacial organs are highly diverse and exert fundamental and specific functions such as breathing, chewing, speech, smell, and sight (Mao et al., 2012). The physiological functions of these organs are compromised by traumatic injuries, congenital and infectious diseases, and cancer (Mao et al., 2012;Scheller et al., 2009). Furthermore, these pathologies are often accompanied by intensive pain and aesthetic deformities. Therefore, the treatment of compromised pathological orofacial tissues and organs should guarantee restoration of both functionality and aesthetics, which constitutes an enormous clinical challenge. Moreover, organ structure, function, aesthetics, and pain should be managed simultaneously during the regenerative care, a situation that is more complex than in other compartments of the body (Scheller et al., 2009).Biological regeneration is proving an increasingly attractive alternative and complement to traditional surgical techniques for prosthetic replacement of tissues and organs. Cell-based therapeutic approaches are already applied with success in clinics and consist of in vitro manipulation of stem cells and their consequent administration to patients as living and dynamic biological agents. Stem cells are characterised by their potential to self-replicate and their capacity to differentiate into a vast variety of cell types that form the diverse tissues. Therefore, stem cells guarantee tissue repair and regeneration throughout life. During the last decades, a plethora of adult stem cell populations have been isolate...

show abstract

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Pagella

Neto

Lamghari

et al. 2015

eCM

View full text Add to dashboard Cite

show abstract

“…3,9,[13][14][15][16][17] Many reports have described the multi-potentiality and possible applications of mesenchymal DSCs in dental and non-dental tissue regeneration. [18][19][20][21][22][23] [18][19][20][21][22][23][24] Their uniqueness of development from CNCCs explains their multilineage differentiation characteristics.…”

Section: Dental Stem Cells (Dscs)mentioning

confidence: 99%

“…68 The regeneration of non-dental tissues of critical value, such as the cornea, cardiac cells, neurons, pancreatic islets, and retina, from oral and DSCs will have favorable results in the near future because of their embryonic origin. [18][19][20][21][22][23]43 The current treatment protocols for dental defects by either the fillings with biocompatible materials or the tooth replacement with dental implants are well established with standard techniques, which are economically affordable and ethically safe. Clinical studies on human dental tissue regeneration should target acceptable and easy procedures for application in regenerative medicine with nano-structured materials as reported by Mitsiadis et al 69 and Mitsiadis and Papagerakis.…”

Section: Oepscs In Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

Odontogenic epithelial stem cells: hidden sources

Priya

Higuchi

Fanas

et al. 2015

Laboratory Investigation

View full text Add to dashboard Cite

The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering

Chuenjitkuntaworn

Osathanon

Nowwarote

et al. 2015

J Biomedical Materials Res

View full text Add to dashboard Cite

Major drawbacks of using an autograft are the possibilities of insufficient bony source and patient's morbidity after operation. Bone tissue engineering technology, therefore, has been applied for repairing bony defects. Previous study showed that a novel fabricated 3D-Polycaprolactone/Hydroxyapatite (PCL/HAp) scaffold possessed a good biocompatibility for bone cells. This study aimed to determine the ability of PCL/HAp for supporting cell growth, gene expression, and osteogenic differentiation in three types of mesenchymal stem cells, including bone marrow-derived mesenchymal stem cells (BMSCs), dental pulp stem cells (DPSCs), and adiposed-derived mesenchymal stem cells (ADSCs). These were assessed by cell viability assay (MTT), reverse-transcription polymerase chain reaction (RT-PCR) analysis, alkaline phosphatase activity, and osteogenic differentiation by alizarin red-S staining. The results showed that PCL/HAp scaffold could support growth of all three types of mesenchymal stem cells. In addition, DPSCs with PCL/HAp showed the highest level of calcium deposition compared to other groups. In conclusion, DPSCs exhibited a better compatibility with these scaffolds compared to BMSCs and ADSCs. However, the PCL/HAp could be a good candidate scaffold for all tested mesenchymal stem cells in bone tissue engineering.

show abstract

Dental pulp stem cells: State of the art and suggestions for a true translation of research into therapy

Cited by 160 publications

References 72 publications

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Odontogenic epithelial stem cells: hidden sources

The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering

Contact Info

Product

Resources

About