Dentin Barrier Test with Transfected Bovine Pulp-Derived Cells

Schmalz, Gottfried; Schuster, Uta; Thonemann, Birger; Barth, Marion; Esterbauer, Sandra

doi:10.1097/00004770-200102000-00009

Cited by 38 publications

(37 citation statements)

References 15 publications

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“…Dentin permeability is of special interest for adhesion of materials to dentin and for their biological effect on the pulp 35) . Dentin acts as a diffusion and adsorption barrier, thus reducing the concentration of eluted substances 36) . The residual dentin layer absorbs unbound monomers and, therefore, contributes to the decrease in the cytotoxicity of the material 37) .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

et al. 2011

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This study evaluated the cytotoxicity of self-etching primers/adhesives by direct contact and dentin barrier tests. The three two-step self-etching systems Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Prime&Bond NT/NRC (PB) and one-step self-etching systems Reactmer Bond (RB), Clearfil Tri-S Bond (CTS), and Adper Prompt L-Pop (AP) were examined. In direct contact tests, L929 cells were cultured in the presence of diluted solutions (50, 20, 10, and 1%) of primer/conditioner of adhesive systems. For dentin barrier tests, each system was applied onto 0.5 or 1.5 mm thick human dentin assembled in a simple pulp chamber device and incubated for 24 h at 37°C to make the diffusive components contact the L929 cells placed at the bottom of the chamber. The cytotoxic effects were assessed by MTT assay. Cell culture without application of any primers/adhesives served as the control for both tests. One-way ANOVA and Tukey HSD tests were used for statistical analyses. The direct contact tests demonstrated that CSE and CPB were less toxic than the other materials at all dilutions. In the dentin barrier tests, toxic effects of materials were reduced with an increase in thickness of intervening dentin. CSE and CPB showed less cytotoxicity than the other adhesives (p<0.05) when applied to 0.5 mm-thick dentin, and CSE was the least toxic in the 1.5 mm-dentin group (p<0.05). Dentin thickness positively affected biocompatibility of the tested bonding systems. Two-step self-etching systems with HEMA-based primers were more biocompatible than other self-etching adhesives.

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Section: Discussionmentioning

confidence: 99%

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

et al. 2011

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“…17,18 Hücre kültür test yöntemleri, hayvan deneylerine kıyasla uygulaması daha kolay, daha az zaman alan ve daha ekonomik testlerdir. 16 Hücre kültür yöntemlerinde yaygın olarak kullanılan MTT testinin güvenilirliği yapılan çalışmalarda belirtilmiştir.…”

Section: Discussionunclassified

Dental Rezi̇n Kompozi̇tleri̇n Si̇totoksi̇si̇tesi̇: Bi̇r i̇n Vi̇tro

Demirci¹,

Gürbüz²,

Şengül³

2015

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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“…Sığır dişi kullanılması ise; istenilen miktarda elde edilebilmesi ve geçirgenlik bakımından insan dentinine kıyasla daha az çeşitlilik göstermesi açısından faydalı olmaktadır. 16,35,36 …”

Section: Dentin Bariyer Test Yöntemiunclassified