Dentin Phosphophoryn Promotes Cellular Migration of Human Dental Pulp Cells

Abstract

Dentin phosphophoryn (DPP) is a dentin sialophosphoprotein gene product that has an RGD motif and repeat sequences of aspartic acid and phosphoserine. To date, the function of DPP in the early stage of reparative dentin formation still remains unclear. The objective of this study was to evaluate the effects of DPP on pulp cell migration and proliferation. DPP promoted cell migration in a concentration-dependent manner, thus increasing it by about 3-fold at 1000 ng/mL compared with the control, but it had no effect on cell proliferation. Dephosphorylated DPP also promoted cell migration, similarly to DPP. However, cell migration was significantly suppressed by the addition of alphavbeta3 integrin antibody to the medium. Furthermore, porcine DPP-derived RGD peptide, but not its mutant RAD peptide, significantly promoted cell migration. These results indicated that the RGD motif of DPP plays an important role in the migration of human dental pulp cells.

The Effect of Matrix Extracellular Phosphoglycoprotein and Its Downstream Osteogenesis-related Gene Expression on the Proliferation and Differentiation of Human Dental Pulp Cells

“…It is reported that dentin phosphophoryn promoted differentiation and cell migration by acting on integrins on the surface of DPCs via RGD motif (32). Furthermore, both osteogenic markers DMP-1 and OPN share the same integrin receptorbinding site during cell attachment in vitro, whereas integrins aVb3 or aVb5 can promote cell attachment to BSP, suggesting there might be an interaction between osteogenic markers and integrins acting on cell adhesion and migration (33).…”

The Antimicrobial Peptide LL37 Induces the Migration of Human Pulp Cells: A Possible Adjunct for Regenerative Endodontics

“…Therefore, using LL37 as a pulp-capping agent on reversibly inflamed pulp may be effective for pulp healing and the formation of reparative dentin. Dentin phosphophoryn also promotes HP cell migration (31). Its traditional role is to induce dentin calcification (32).…”

Dentin sialophosphoprotein and dentin matrix protein-1: Two highly phosphorylated proteins in mineralized tissues

“…This suggests that DPP activates Smad-1 via integrin signaling independently of BMP signaling (52). Also, an RGD peptide derived from porcine DPP promotes cellular migration of human dental pulp cells (53). Taken together, these observations indicate that DSPP functions as a cell-signaling molecule.…”