Deoxynivalenol affects in vitro intestinal epithelial cell barrier integrity through inhibition of protein synthesis

Walle, Jacqueline Van De; Sergent, Thérèse; Piront, Neil; Toussaint, Olivier; Schneider, Yves Jacques; Larondelle, Yvan

doi:10.1016/j.taap.2010.03.012

Cited by 159 publications

(121 citation statements)

References 48 publications

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“…Furthermore, the study also showed the intravenous lethality of DON, while in another study, De Walle et al (11) demonstrated a dual toxicological effect of DON on differentiated Caco-2 cells consisting of an inhibition of protein synthesis as well as an increase in monolayer permeability. The toxicity of DON in domestic animals is well documented.…”

Section: Discussionmentioning

confidence: 87%

Technetium-99m-labeled deoxynivalenol from Fusarium mycotoxin alters organ toxicity in BALB/c mice by oral and intravenous route

Chattopadhyay¹,

Pandey²,

Goyary³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:The toxicity of deoxynivalenol, both intravenously and orally, was investigated in male and female BALB/c mice. Technetium-99m ( 99m Tc)-labeled deoxynivalenol was administered to mice by tail vein injection and orally dosed. Distribution of labeled deoxynivalenol at 26 hours was monitored by gammascintigraphy. In the evaluated organs, the accumulation of radioactive deoxynivalenol was correlated with the amount of radioactivity. In addition, the toxicity of deoxynivalenol was measured by biochemical assays followed by histopathological findings. Kidney and hepatic marker enzymes were significantly increased in intravenously administered deoxynivalenol as compared to orally treated mice. Intravenously treated mice showed severe damage in liver and kidney when compared to those orally exposed. Biodistribution of 99m Tclabeled deoxynivalenol differed between oral and intravenous treatment. In intravenously exposed mice, deoxynivalenol was distributed primarily in the liver and kidney whereas in oral exposure, it was found in the stomach and intestines after 26 hours. Deoxynivalenol toxicity, associated with its biodistribution and organ toxicity, was greatest where it had accumulated. The results show that the toxicity of deoxynivalenol is associated with organ accumulation.

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Section: Discussionmentioning

confidence: 87%

Technetium-99m-labeled deoxynivalenol from Fusarium mycotoxin alters organ toxicity in BALB/c mice by oral and intravenous route

Chattopadhyay¹,

Pandey²,

Goyary³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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“…The decrease in protein synthesis is the primary mechanism by which mycotoxins reduce weight gain in animals. This interference is observed in intoxications by aflatoxins and trichothecenes (Lindemann et al, 1993;Lin et al, 2006;Walle et al, 2010). Pigs fed with diets containing aflatoxins presented reduction in the energy metabolism and in nitrogen retention (Hauschild et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Meta-analytical study of productive and nutritional interactions of mycotoxins in growing pigs

Andretta

Kipper

Lehnen

et al. 2012

Animal

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A meta-analysis was carried out in order to study the association of mycotoxins with performance and organ weights in growing pigs. A total of 85 articles published between 1968 and 2010 were used, totaling 1012 treatments and 13 196 animals. The metaanalysis followed three sequential analyses: graphical, correlation and variance-covariance. The presence of mycotoxins in diets was seen to reduce the feed intake by 18% and the weight gain in 21% compared with the control group. Deoxynivalenol and aflatoxins were the mycotoxins with the greatest impact on the feed intake and growth of pigs, reducing by 26% and 16% in the feed intake and by 26% and 22% in the weight gain. The mycotoxin concentration in diets and the animal age at challenge were the variables that more improved the coefficient of determination in equations for estimating the effect of mycotoxins on weight gain. The mycotoxin effect on growth proved to be greater in younger animals. In addition, the residual analysis showed that the greater part of the variation in weight gain was explained by the variation in feed intake (87%). The protein and methionine levels in diets could influence the feed intake and the weight gain in challenged animals. The weight gain in challenged pigs showed a positive correlation with the methionine level in diets (0.68). The mycotoxin effect on growth was greater in males compared with the effect on females. The reduction in weight gain was of 15% in the female group and 19% in the male group. Mycotoxin presence in pig diets has interfered in the relative weight of the liver, the kidneys and the heart. Mycotoxins have an influence on performance and organ weight in pigs. However, the magnitude of the effects varies with the type and concentration of mycotoxin, sex and the animal age, as well as nutritional factors.Keywords: aflatoxins, deoxynivalenol, nutrition, swine, trichothecenes ImplicationsThe mycotoxin effect on animal performance is recognized. However, this effect may be modified by several factors, which are noticeable in the large variability observed in previous results. This study is innovative, because it seeks to understand and quantify the interactions among mycotoxins and other factors such as nutrition, sex or growth phase of the pigs. The meta-analysis used the complementarities among previous studies in order to highlight gaps in mycotoxicology, which is hardly studied in traditional experimental designs. Furthermore, it aimed to assist in understanding the mycotoxin effects that this approach can support in the determination of dynamic growth standards for challenged animals.

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“…At the cellular level, DON causes cell death via apoptosis and inhibits protein synthesis by obstructing translation at the ribosomal level, leading to ribotoxic stress syndrome (reviewed by Pestka [2010]). These effects are known to have the greatest impact on rapidly dividing cells, such as epithelial and immune cells in the gastrointestinal tract (GIT; Van De Walle et al, 2010). Thus, DON contamination causes compromised barrier function by decreasing the expression of tight junction proteins (Van De Walle et al, 2010;Pinton et al, 2012) and can increase the susceptibility of the GIT to bacterial infections (Grenier and Applegate, 2013).…”

Section: Discussionmentioning

confidence: 99%

The progression of deoxynivalenol-induced growth suppression in nursery pigs and the potential of an algae-modified montmorillonite clay to mitigate these effects12

Frobose

Erceg

Fowler

et al. 2016

Journal of Animal Science

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Two experiments were conducted to characterize the progression of deoxynivalenol (DON)-induced growth suppression and to investigate algae-modified montmorillonite clay (AMMC) as a means to alleviate the effects of DON in nursery pigs. In both experiments, naturally DON-contaminated wheat was used to produce diets with desired DON levels. In Exp. 1, 280 barrows and gilts (10.0 ± 0.2 kg BW) were used in a 28-d experiment arranged in a 2 × 2 + 1 factorial design with 8 replicates per treatment. The 5 treatments consisted of 2 positive control (PC) diets with DON below detection limits and with or without 0 or 0.50% AMMC and 3 negative control (NC) diets with 5 mg/kg of DON and containing 0, 0.25, or 0.50% AMMC. No DON × AMMC interactions were observed. Overall, pigs fed DON had decreased (P < 0.001) ADG and final BW regardless of AMMC addition. Feeding DON-contaminated diets elicited the most severe depression (P < 0.001) in ADFI and G:F from d 0 to 3, remaining poorer overall (P < 0.01) but lessening in severity as exposure time increased. Pigs fed DON diets had greater (P < 0.05) within pen BW variation (CV) on d 28. Although the addition of 0.50% AMMC to diets restored (P < 0.05) ADFI from d 14 to 21 to levels similar to the PC, no other differences were observed for AMMC inclusion. In Exp. 2, 360 barrows (11.4 ± 0.2 kg BW) were used in a 21-d experiment with 9 dietary treatments arranged in a 3 × 3 factorial design with DON and AMMC inclusion as main effects. There were 8 replicate pens per treatment. Treatments consisted of 3 PC diets without DON, 3 low-DON (1.5 mg/kg DON) NC diets, and 3 high-DON (3 mg/kg DON) NC diets with 0, 0.17, or 0.50% AMMC incorporated at each DON level. No DON × AMMC interactions were observed. As DON level increased, ADG and final BW decreased (quadratic, P < 0.05), driven by decreased (quadratic, P < 0.01) ADFI and poorer (quadratic; P < 0.05) G:F. At both 1.5 and 3 mg/kg DON, reductions in ADG were most marked from d 0 to 7 (15 to 22% lower) and were least distinct from d 14 to 21 (5 to 6% lower). Incorporating AMMC at increasing levels had no effect on ADG, ADFI, G:F, or final BW. Overall, these experiments reinforce DON effects on feed intake but also indicate that the effects of DON on G:F may be more severe than previously thought. Furthermore, some pigs appear to develop tolerance to DON, as effects on ADFI and G:F lessen over time. However, the addition of AMMC did not offset the deleterious effects of DON.

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Deoxynivalenol affects in vitro intestinal epithelial cell barrier integrity through inhibition of protein synthesis

Cited by 159 publications

References 48 publications

Technetium-99m-labeled deoxynivalenol from Fusarium mycotoxin alters organ toxicity in BALB/c mice by oral and intravenous route

Technetium-99m-labeled deoxynivalenol from Fusarium mycotoxin alters organ toxicity in BALB/c mice by oral and intravenous route

Meta-analytical study of productive and nutritional interactions of mycotoxins in growing pigs

The progression of deoxynivalenol-induced growth suppression in nursery pigs and the potential of an algae-modified montmorillonite clay to mitigate these effects12

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