Deploying a microbial consortium of Serendipita indica, Rhizophagus intraradices, and Azotobacter chroococcum to boost drought tolerance in maize

Tyagi, Jaagriti; Mishra, Arti; Kumari, Shilpi; Singh, Sakshi; Agarwal, Himani; Pudake, Ramesh Namdeo; Varma, Ajit; Joshi, Naveen Chandra

doi:10.1016/j.envexpbot.2022.105142

Cited by 28 publications

(13 citation statements)

References 57 publications

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“…In fact, the promoting effect of AMF depends on the AMF species and their hosts ( Diagne et al., 2020 ; Ran et al., 2021a ; Amani Machiani et al., 2022 ). R. irregularis , F. mosseae and C. etunicatum are common AMF used to improve the contents of active substances and strengthen tolerance to stress ( Ghanbarzadeh et al., 2019 ; Yilmaz and Karik, 2022 ; Tyagi et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Dai,

Zhang,

et al. 2024

Front. Plant Sci.

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Panax notoginseng is a highly valued perennial medicinal herb plant in Yunnan Province, China, and the taproots are the main medicinal parts that are rich in active substances of P. notoginseng saponins. The main purpose of this study is to uncover the physiological and molecular mechanism of Panax notoginseng saponin accumulation triggered by methyl jasmonate (MeJA) under arbuscular mycorrhizal fungi (AMF) by determining physiological indices, high-throughput sequencing and correlation analysis. Physiological results showed that the biomass and saponin contents of P. notoginseng, the concentrations of jasmonic acids (JAs) and the key enzyme activities involved in notoginsenoside biosynthesis significantly increased under AMF or MeJA, but the interactive treatment of AMF and MeJA weakened the effect of AMF, suggesting that a high concentration of endogenous JA have inhibitory effect. Transcriptome sequencing results indicated that differential expressed genes (DEGs) involved in notoginsenoside and JA biosynthesis were significantly enriched in response to AMF induction, e.g., upregulated genes of diphosphocytidyl-2-C-methyl-d-erythritol kinases (ISPEs), cytochrome P450 monooxygenases (CYP450s)_and glycosyltransferases (GTs), while treatments AMF-MeJA and salicylhydroxamic acid (SHAM) decreased the abundance of these DEGs. Interestingly, a high correlation presented between any two of saponin contents, key enzyme activities and expression levels of DEGs. Taken together, the inoculation of AMF can improve the growth and saponin accumulation of P. notoginseng by strengthening the activities of key enzymes and the expression levels of encoding genes, in which the JA regulatory pathway is a key link. This study provides references for implementing ecological planting of P. notoginseng, improving saponin accumulation and illustrating the biosynthesis mechanism.

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Section: Discussionmentioning

confidence: 99%

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Dai,

Zhang,

et al. 2024

Front. Plant Sci.

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“…Microbial interactions are crucial for promoting plant growth in the rhizospheric soil. A combination of S. indica , A. chroococcum , and Rhizophagus intraradices , increased the growth of Zea mays by increasing shoots, roots, and biomass accumulation ( Tyagi et al., 2023 ). Co-inoculation of S. indica and A. chroococcum has been shown to boost Stevia rebaudiana growth by enhancing photosynthetic molecule biosynthesis, resulting in increased chlorophyll, carotenoids, total sugar, and protein levels ( Kumar Bhuyan et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Interaction studies of Serendipita indica and Zhihengliuella sp. ISTPL4 and their synergistic role in growth promotion in rice

et al. 2023

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Rapid urbanization and globalization demand increasing agricultural productivity. Soil nutrient supply capacity is continuously decreasing due to soil erosion, degradation, salt deposition, undesired element, metal deposition, water scarcity, and an uneven nutrient delivery system. Rice cultivation requires a large amount of water which is becoming detrimental due to these activities. There is a need to increase its productivity. Microbial inoculants are becoming increasingly important in achieving sustainable agricultural production systems. The current study was conducted to investigate the interaction between the root endophytic fungus Serendipita indica (S. indica) and the actinobacterium Zhihengliuella sp. ISTPL4 (Z. sp. ISTPL4) and their synergistic effects on the growth of rice (Oryza sativa L). Both S. indica and Z. sp. ISTPL4 showed positive interactions. Growth of S. indica was observed at different days after Z. sp. ISTPL4 inoculation, and stimulated growth of S. indica was observed when Z. sp. ISTPL4 was inoculated at 5 dafi (days after fungal inoculation). Z. sp. ISTPL4 promoted the growth of S. indica as it increased spore germination. Furthermore, confocal and scanning electron microscopy (SEM) analyses showed a 27% increase in the spore size of S. indica in the presence of Z. sp. ISTPL4. In a liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis increased production of alanine and glutamic acid was observed in their sequential co-culture as compared with individual cultures. Sequential inoculation of S. indica and Z. sp. ISTPL4 significantly increased the biochemical and physical characteristics of rice as compared with their individual inoculum. Biochemical parameters such as chlorophyll content, total soluble sugar, and flavonoid content in the rice increased by up to 57%, 47%, and 39%, respectively, in the presence of the combined inoculum of S. indica and Z. sp. ISTPL4. This will be the first study, to the best of our knowledge, which shows the fungus and actinobacterium interaction and their synergistic roles in the growth promotion of rice. Furthermore, this novel combination can also be used to boost the growth of other crops to increase the agricultural yield.

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“…Microorganism inoculation not only improves plant growth, but also ameliorates the deleterious effect of Cd and drought stress by regulating antioxidant activities and secondary metabolite accumulation in plants [45]. The improvements in the biochemical rate of CO 2 fixation that resulted from the increase in photosynthetic pigment content was deemed to be crucial for boosting the net photosynthesis and the growth rate of plants under abiotic stress [62]. Since the PGPB was found to significantly affect the metal bioavailability and its uptake by plants through a variety of mechanisms, including metal reduction and oxidation, metal methylation, formation of insoluble complexes, chelation through siderophores, bioaccumulation, and intracellular sequestration, the role of PGPB in improving heavy metal tolerance under drought stress conditions is noteworthy [9].…”

Section: Discussionmentioning

confidence: 99%

Morphophysiological and Biochemical Responses of Zea mays L. under Cadmium and Drought Stresses Integrated with Fungal and Bacterial Inoculation

et al. 2023

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Cadmium and drought stress are the most destructive of the abiotic stresses with negative effects on both metabolism and photosynthesis. The present experiment aimed to analyze the impact of inoculation with Bacillus paralicheniformis and Trichoderma asperellum on the growth and antioxidant response modulation of maize (Zea mays L.), under drought and Cadmium (Cd) stresses. Regarding plant biomass analysis, fungi inoculation increased leaf dry biomass significantly (11.92%) towards uninoculated ones. The leaf area was affected significantly by bacterial application, 12.15% more than the control. A significant trend (drought+ Cd stress) was observed between fungi-inoculated maize leaves (15.07 µmol/g FW) and bacterial-inoculated leaves (18.71 µmol/g FW) regarding the malondialdehyde quantity. Furthermore, the activities of superoxide dismutase were notably higher (9.63–40.88%) in microorganism-inoculated roots. Similarly, under drought + Cd stress, peroxidase demonstrated a higher activity under bacterial inoculation than fungal ones (92.11% more). The maximum translocation factor was observed in the uninoculated group (under Cd stress), while the bioconcentration factor under drought stress showed a significant increase by microorganisms. The maximum relative water content under bacterial inoculation (82.66%) was achieved. The fungi and bacterial inoculation minimized Cd accumulation in the leaf significantly under drought and drought + Cd stress. Generally, the microorganism inoculation positively and partially maintained the plant’s performance, despite the presence of drought and Cd stress.

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Deploying a microbial consortium of Serendipita indica, Rhizophagus intraradices, and Azotobacter chroococcum to boost drought tolerance in maize

Cited by 28 publications

References 57 publications

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Interaction studies of Serendipita indica and Zhihengliuella sp. ISTPL4 and their synergistic role in growth promotion in rice

Morphophysiological and Biochemical Responses of Zea mays L. under Cadmium and Drought Stresses Integrated with Fungal and Bacterial Inoculation

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