Deproteinated bovine bone vs. beta‐tricalcium phosphate as bone graft substitutes: histomorphometric longitudinal study in the rabbit cranial vault

Martínez, A.; Balboa, Óscar; Gasamans, Iria; Otero-Cepeda, Xose Luis; Guitián, Francisco

doi:10.1111/clr.12349

Cited by 23 publications

(30 citation statements)

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“…This is consistent with our understanding that biomaterials used for bone augmentation delay the healing and maturation of bone tissue [25]. Similar healing pattern can be found in some animal studies with various biomaterials and duration of follow-up, although they did not observe the effect of LMHFV [26]. A histomorphometric study in the rabbit cranial vault showed higher mean bone fraction after 1 week in sites treated with deproteinized bovine bone compared to membrane only, control sites.…”

Section: Discussionsupporting

confidence: 88%

The effect of local application of low-magnitude high-frequency vibration on the bone healing of rabbit calvarial defects—a pilot study

Puhar

Suleimenova

et al. 2016

J Orthop Surg Res

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BackgroundThe objective of this pilot study was to evaluate the effect of local application of low-magnitude high-frequency vibration (LMHFV) on the bone healing of rabbit calvarial defects that were augmented with different grafting materials and membranes.MethodsFour calvarial defects were created in each of two New Zealand rabbits and filled with the following materials: biphasic calcium phosphate (BCP), deproteinized bovine bone mineral covered with a non-cross-linked collagen membrane (BO/BG), biphasic calcium phosphate covered with a strontium hydroxyapatite-containing collagen membrane (BCP/SR), and non-cross-linked collagen membrane (BG). Four defects in one rabbit served as a control, while the other was additionally subjected to the local LMHFV protocol of 40 Hz, 16 min per day. The rabbits were sacrificed 1 week after surgery. Histomorphometric analysis was performed to determine the percentages of different tissue compartments.ResultsCompared to the control defects, the higher percentage of osteoid tissue was found in LMHFV BG defects (35.3 vs. 19.3%), followed by BCP/SR (17.3 vs. 2.0%) and BO/BG (9.3 vs. 1.0%). The fraction occupied by the residual grafting material varied from 40.3% in BO/BG to 22.3% in BCP/SR LMHFV defects. Two-way models revealed that material type was only significant for the osteoid (P= 0.045) and grafting material (P = 0.001) percentages, while the vibration did not provide any statistical significance for all histomorphometric outcomes (P > 0.05).ConclusionLocal application of LMHFV did not appear to offer additional benefit in the initial healing phase of rabbit calvarial defects. Histomorphometric measurements after 1 week of healing demonstrated more pronounced signs of early bone formation in both rabbits that were related with material type and independent of LMHFV.

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Section: Discussionsupporting

confidence: 88%

The effect of local application of low-magnitude high-frequency vibration on the bone healing of rabbit calvarial defects—a pilot study

Puhar

Suleimenova

et al. 2016

J Orthop Surg Res

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“…In this study, the volume of residual graft in all four groups were similar and slightly decreased from 6 to further 12 weeks. This is also in agreement with other experimental studies that observed a long‐term permanence of bovine derived bone grafts . They are generally regarded as two material degradation mechanisms: cell‐mediated dissolution and cell‐mediated resorption .…”

Section: Resultssupporting

confidence: 91%

“…These limitations have motivated broad interests in searching for alternatives. Deproteinized bovine‐derived mineral has long been viable alternative bone grafts to autografts and has proven to have excellent performance in bone regeneration …”

Section: Introductionmentioning

confidence: 99%

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The optimization of sintering treatment on bovine‐derived bone grafts for bone regeneration: in vitro and in vivo evaluation

Lin

et al. 2019

J Biomed Mater Res

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Modifications of sintering temperature and treatment time of bovine‐derived bone grafts affect their physicochemical properties and further influence biological activity. Three different temperature sintered bovine‐derived bone grafts: group I (300 °C 3 h), group II (300 °C 3 h plus 530 °C 6 h), and group III (300 °C 3 h plus 1000 °C 2 h) and Bio‐Oss® were characterized and then compared in vitro for their effects on bone marrow stromal cells (BMSCs) migration, proliferation, and differentiation as estimated by cell migration assay, Alkaline phosphatase (ALP) activity assay, and Alizarin red staining. Further, the four bone grafts were implanted into the calvarial defects of rabbits to evaluate bone regeneration and graft degradation. The four deproteinized bovine‐derived bone grafts displayed different surface topography. Group II displayed the highest potential of attracting cells. Both groups I and II markedly promote BMSCs differentiation. After 6 and 12 weeks, defects grafted with groups I and II displayed a significant higher bone fraction than defects grafted with group III and Bio‐Oss®. Bone graft remnants remained in all four groups. Taken together, sintering at 300 °C for 3 h and sintering at 300 °C for 3 h with an addition of 530 °C for 6 h of bovine‐dervied bone grafts displayed potential use in bone regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:272–281, 2020.

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“…En el mercado actual existen sustitutos óseos que han superado algunas barreras iniciales para su aplicación (Martinez et al, 2015). Sin embargo, no existe suficiente información que permita establecer algún tipo de material no autógeno como viable de obtener regeneración ósea en condiciones normales del sistema estomatognático humano o en modelos in vivo (Rezwan et al, 2006;Hutmacher et al, 2007).…”

Section: Introductionunclassified

Modelo Experimental para el Estudio de la Reconstrucción Mandibular: Opciones en Conejos Oryctolagus cuniculus

et al. 2017

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Morphol., 35(3):1185Morphol., 35(3): -1190Morphol., 35(3): , 2017. RESUMEN:El propósito de este estudio fue de evaluar la técnica quirúrgica propuesta para estudios de resección y reconstrucción mandibular en modelos experimentales en conejos. Se utilizaron 7 conejos neozelandeses (Orictolagus cuniculus), en los cuales se realizó la resección del cuerpo mandibular izquierdo, una vez asegurada la movilidad de los segmentos, se reconstruyó con placa de osteosíntesis de titanio de 1,5 mm y tornillos monocorticales de 5 mm, en la zona resecada se fijó un bloque de beta fosfato tricálcico y estabilizado con tornbillo de 1,2 mm. Se sacrificaron los animales a los 2 meses. Todos los animales sobrevivieron al procedimiento y se pudo llevar a cabo la resección y reconstrucción en todos ellos. No se presentaron signos ni síntomas de infección en el sitio quirúrgico mientras duró el experimento. El tiempo promedio de cirugía fue de 68 minutos. El peso promedio de los animales fue de 3925 g, la pérdida promedio de peso fue de 2,03 %. No se realizó eutanasia de ningún animal por motivos de sufrimiento. Al examen macroscópico de las muestras extraídas se observó la presencia de todos los elementos de osteosíntesis en posición, así como continuidad de la estructura ósea casi en su totalidad en la superficie . La utilización de conejos en modelos de reconstrucción maxilofacial es un modelo probado debido a la similitud de los procesos reparativos, su facilidad de manipulación y cuidados. El modelo propuesto representa una alternativa más cercana a los procesos reconstructivos en cirugía resectiva maxilofacial, debido a que somete los injertos a las fuerzas propias de la masticación y de la función orofacial.PALABRAS CLAVE: Injerto óseo; Scaffold; Ingeniería de tejidos; β β β β β-TCP.

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Deproteinated bovine bone vs. beta‐tricalcium phosphate as bone graft substitutes: histomorphometric longitudinal study in the rabbit cranial vault

Cited by 23 publications

References 35 publications

The effect of local application of low-magnitude high-frequency vibration on the bone healing of rabbit calvarial defects—a pilot study

The effect of local application of low-magnitude high-frequency vibration on the bone healing of rabbit calvarial defects—a pilot study

The optimization of sintering treatment on bovine‐derived bone grafts for bone regeneration: in vitro and in vivo evaluation

Modelo Experimental para el Estudio de la Reconstrucción Mandibular: Opciones en Conejos Oryctolagus cuniculus

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