Deproteinized bovine bone remodeling pattern in alveolar socket: a clinical immunohistological evaluation

Milani, Stefano; Pozzo, Laura Dal; Rasperini, Giulio; Sforza, Chiarella; Dellavia, Claudia

doi:10.1111/clr.12535

Cited by 21 publications

(19 citation statements)

References 33 publications

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“…Similarly, [26] reported 57.75% of non-vital bone when using autogenous bone blocks. In the present investigation CCXBB was present in 21.4% of the samples after 26 weeks of healing, what is in agreement with previous studies reporting histological outcomes of other xenogeneic bone replacement grafts placed for the regeneration of extraction sockets [27, 28]. In this indication, the percentage of residual graft was 39.8 and 33.4%, respectively.…”

Section: Discussionsupporting

confidence: 92%

“…ALP-positive cells were detectable, in all specimens on the periphery of MVB, associated to areas of new bone formation. These observations were also reported on a clinical study on guided bone regeneration (GBR) [41], as well as through the evaluation of the healing of particulate xenogeneic bone grafts (DBBM) [28]. Experimental research using immune-histochemical analysis for comparing early bone remodelling between autografts and allografts has reported comparable behavior for osteoprotegerin (OPG), alkaline phosphatase (ALP), collagen 1 (COLI), osteopontin (OPN), and osteocalcin (OSC), although an increased activity of tartrate-resistant acid phosphatase (TRAP) was seen in allogenic bone grafts [42].…”

Section: Discussionmentioning

confidence: 75%

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Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement

et al. 2017

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BackgroundThe osteoconductive properties of collagen containing xenogeneic bone blocks (CCXBB) remain unclear. The aim of this prospective single-arm clinical study was to assess the histological outcomes of CCXBB blocks used as bone replacement grafts for lateral bone augmentation procedures.MethodsIn 15 patients with severe horizontal alveolar ridge resorption, lateral augmentation procedures were performed using CCXBB as bone replacement grafts. Twenty-six weeks postoperatively, a re-entry procedure was performed to evaluate the bone width for adequate implant placement and two histological specimens were retrieved from each patient, one being processed for ground sectioning and the other for decalcified paraffin-included sections. In non-decalcified sections, the relative proportions occupied by bone, biomaterials, and connective tissue present in the biopsies were identified. In de-calcified sections, structures and cells positive for osteopontin (OPN), tartrate-resistant acid phosphatase activity (TRAP), osteocalcin (OSC), and alkaline phosphatase (ALP) were assessed.ResultsSoft tissue dehiscence occurred during the follow-up in 5 out of 15 patients (33.3%). The mean crest width at baseline was 2.78 mm (SD 0.57) and the mean crest width at re-entry was 6.90 mm (SD 1.22), with a mean ridge width increase of 4.12 mm (SD 1.32). Twenty-six bone biopsies were obtained from 13 patients. Histomorphometric analysis showed a mean of 26.90% (SD 12.21) of mineralized vital bone (MVB), 21.37% (SD 7.36) of residual CCXBB, 47.13% (SD 19.15) of non-mineralized tissue, and 0.92% of DBBM. The immunohistochemical analysis revealed a large number of OPN-positive cells 8.12% (SD 4.73), a lower proportion of TRAP positive multinuclear cells 5.09% (SD 4.91), OSC-positive cells 4.09% (SD 4.34), and a limited amount of ALP positive cells 1.63% (SD 2).ConclusionsCCXBB achieved significant horizontal crestal width allowing for staged implant placement in most of the patients. In light of the histological outcomes and implant failures, special attention must be placed to prevent soft tissue dehiscence when CCXBB is used in severe atrophic alveolar crests.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 75%

Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement

et al. 2017

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“…Milani and colleagues have recently reported immunohistochemical expression of BMP2 and BMP7, not only intracellular, but also around xenograft particles in a socket preservation model in humans. 23 This phenomenon has been related in the literature regarding other proteins expression, as osteopontin or TRAP-1. 24 In the present study, regarding the expression of BMP-4 in function of the different grafting biomaterials studied (i.e., xenograft and allograft), it was observed that the remaining particles of anorganic bovine xenograft got impregnated with this protein, which might be related to the superior rate of bone formation promoted by this biomaterial as compared with the allograft alone.…”

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 71%

Significance of the Immunohistochemical Expression of Bone Morphogenetic Protein‐4 in Bone Maturation after Maxillary Sinus Grafting in Humans

Torrecillas-Martínez

Galindo‐Moreno

Ávila‐Ortiz

et al. 2015

Clin Implant Dent Rel Res

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“…Newly formed tissues were consistently in close proximity to DBBM, which is one of the most investigated scaffolds and is widely employed in regenerative procedures, both in animal models and in clinical practice [2829]. Many case series reported good clinical outcomes from DBBM blocks used in onlay [3031] and inlay procedures [3233].…”

Section: Discussionmentioning

confidence: 99%

“Over-inlay” block graft and differential morphometry: a novel block graft model to study bone regeneration and host-to-graft interfaces in rats

Ghiacci

Graiani

Ravanetti

et al. 2016

J Periodontal Implant Sci

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PurposeThe aim of this study was to present new a model that allows the study of the bone healing process, with an emphasis on the biological behavior of different graft-to-host interfaces. A standardized “over-inlay” surgical technique combined with a differential histomorphometric analysis is presented in order to optimize the use of critical-size calvarial defects in pre-clinical testing.MethodsCritical-size defects were created into the parietal bone of 8 male Wistar rats. Deproteinized bovine bone (DBBM) blocks were inserted into the defects, so that part of the block was included within the calvarial thickness and part exceeded the calvarial height (an “over-inlay” graft). All animals were sacrificed at 1 or 3 months. Histomorphometric and immunohistochemical evaluation was carried out within distinct regions of interest (ROIs): the areas adjacent to the native bone (BA), the periosteal area (PA) and the central area (CA).ResultsThe animals healed without complications. Differential morphometry allowed the examination of the tissue composition within distinct regions: the BA presented consistent amounts of new bone formation (NB), which increased over time (24.53%±1.26% at 1 month; 37.73%±0.39% at 3 months), thus suggesting that this area makes a substantial contribution toward NB. The PA was mainly composed of fibrous tissue (71.16%±8.06% and 78.30%±2.67%, respectively), while the CA showed high amounts of DBBM at both time points (78.30%±2.67% and 74.68%±1.07%, respectively), demonstrating a slow remodeling process. Blood vessels revealed a progressive migration from the interface with native bone toward the central area of the graft. Osterix-positive cells observed at 1 month within the PA suggested that the periosteum was a source of osteoprogenitor elements. Alkaline phosphatase data on matrix deposition confirmed this observation.ConclusionsThe present model allowed for a standardized investigation of distinct graft-to-host interfaces both at vertically augmented and inlay-augmented sites, thus possibly limiting the number of animals required for pre-clinical investigations.

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Deproteinized bovine bone remodeling pattern in alveolar socket: a clinical immunohistological evaluation

Abstract: Deproteinized bovine bone guarantees the activity of bone remodeling processes in human extraction socket at 5 months of healing, bringing to a higher expression of anabolic and catabolic bone markers.

Cited by 21 publications

References 33 publications

Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement

Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement

Significance of the Immunohistochemical Expression of Bone Morphogenetic Protein‐4 in Bone Maturation after Maxillary Sinus Grafting in Humans

“Over-inlay” block graft and differential morphometry: a novel block graft model to study bone regeneration and host-to-graft interfaces in rats

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