Depth resolved label-free multimodal optical imaging platform to study morpho-molecular composition of tissue

Andreana, Marco; Sentosa, Ryan; Erkkilä, Mikael T.; Drexler, Wolfgang; Unterhuber, Angelika

doi:10.1039/c8pp00410b

Cited by 16 publications

(20 citation statements)

References 95 publications

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“…Considering the advantages and drawbacks of the two technologies, it is obvious that 2PM and OCT may complement each other and that their combination would be beneficial for relating information on cellular phenotypes and functions to tissue and organ morphology and, thus, for translating this information to the clinic. Combinations of 2PM and OCT has been previously proposed but only recently demonstrated on biological samples , for example, to correlated skin morphology with lipid‐rich cellular structures . Moreover, previous approaches have been used on different spatial scales and their ability to monitor dynamic changes in tissues at the cellular level has not been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…Optical coherence tomography (OCT), also developed three decades ago, is a true tomographic high‐resolution imaging modality based on the interference of a reference beam and a sample beam that has been reflected by various layers within the tissue . The technology provides image contrast by relying on changes in refractive index within the sample, that is, on the various scattering properties of the tissue.…”

mentioning

confidence: 99%

“…By controlling the laser power in the range of few tens of milliwatts, photodamage is typically reduced to a negligible level for mammalian tissues . Based on elastic reflection and interference, OCT typically employs low coherence light sources at extremely low powers (in the range of microwatts) . Thus, OCT causes practically no damage to the sample and is particularly well suited for in vivo imaging, even of photosensitive tissues such as the retinae.…”

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confidence: 99%

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Coregistered Spectral Optical Coherence Tomography and Two‐Photon Microscopy for Multimodal Near‐Instantaneous Deep‐Tissue Imaging

et al. 2020

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Two-photon microscopy (2PM) has brought unique insight into the mechanisms underlying immune system dynamics and function since it enables monitoring of cellular motility and communication in complex systems within their genuine environment-the living organism. However, use of 2PM in clinical settings is limited. In contrast, optical coherence tomography (OCT), a noninvasive label-free diagnostic imaging method, which allows monitoring morphologic changes of large tissue regions in vivo, has found broad application in the clinic. Here we developed a combined multimodal technology to achieve near-instantaneous coregistered OCT, 2PM, and second harmonic generation (SHG) imaging over large volumes (up to 1,000 × 1,000 × 300 μm 3 ) of tendons and other tissue compartments in mouse paws, as well as in mouse lymph nodes, spleens, and femurs. Using our multimodal imaging approach, we found differences in macrophage cell shape and motility behavior depending on whether they are located in tendons or in the surrounding tissue compartments of the mouse paw. The cellular shape of tissue-resident macrophages, indicative for their role in tissue, correlated with the supramolecular organization of collagen as revealed by SHG and OCT. Hence, the herepresented approach of coregistered OCT and 2PM has the potential to link specific cellular phenotypes and functions (as revealed by 2PM) to tissue morphology (as highlighted by OCT) and thus, to build a bridge between basic research knowledge and clinical observations.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Coregistered Spectral Optical Coherence Tomography and Two‐Photon Microscopy for Multimodal Near‐Instantaneous Deep‐Tissue Imaging

et al. 2020

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“…Hybrid multimodal multiphoton microscopy (MPM) [59] with single-photon sensitivity and submicron spatial resolution using the response of endogenous chemical biomarkers in skin, such as collagen or lipids acts as fast and label-free in vivo optical biopsy [60]. The synergistically combination of OCT with nonlinear optical imaging techniques such as TPEF, SHG, and CARS (Coherent Anti-Stokes Raman Spectroscopy) provides access to detailed information of tissue structure and molecular composition in a fast, label-free and non-invasive manner [61]. MPM offers high axial resolution with molecular contrast but limited speed and penetration depth.…”

Section: Preclinical Hybrid Imagingmentioning

confidence: 99%

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

et al. 2020

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“…In multi-cellular and tissue analysis, RS microscopy may be supplemented by the conjugation of other optical modalities in an intraoperative approach. It is not uncommon to find optical coherence tomography (OCT), FLIM, and CARS images in the same study (Andreana, Sentosa, Erkkilä, Drexler, & Unterhuber, 2019;Shen et al, 2020).…”

Section: Modality Of Raman Imaging On Tissuesmentioning

confidence: 99%

Mammalian cell and tissue imaging using Raman and coherent Raman microscopy

Fung

Shi

2020

WIREs Mechanisms of Disease

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Direct imaging of metabolism in cells or multicellular organisms is important for understanding many biological processes. Raman scattering (RS) microscopy, particularly, coherent Raman scattering (CRS) such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), has emerged as a powerful platform for cellular imaging due to its high chemical selectivity, sensitivity, and imaging speed. RS microscopy has been extensively used for the identification of subcellular structures, metabolic observation, and phenotypic characterization. Conjugating RS modalities with other techniques such as fluorescence or infrared (IR) spectroscopy, flow cytometry, and RNAsequencing can further extend the applications of RS imaging in microbiology, system biology, neurology, tumor biology and more. Here we overview RS modalities and techniques for mammalian cell and tissue imaging, with a focus on the advances and applications of CARS and SRS microscopy, for a better understanding of the metabolism and dynamics of lipids, protein, glucose, and nucleic acids in mammalian cells and tissues.

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Depth resolved label-free multimodal optical imaging platform to study morpho-molecular composition of tissue

Abstract: The presented multi-modal platform combines optical coherence tomography, two-photon excited fluorescence, second harmonic generation and anti-Stokes Raman scattering to provide molecular and structural information of tissue in a fast and non-invasive manner.

Cited by 16 publications

References 95 publications

Coregistered Spectral Optical Coherence Tomography and Two‐Photon Microscopy for Multimodal Near‐Instantaneous Deep‐Tissue Imaging

Coregistered Spectral Optical Coherence Tomography and Two‐Photon Microscopy for Multimodal Near‐Instantaneous Deep‐Tissue Imaging

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

Mammalian cell and tissue imaging using Raman and coherent Raman microscopy

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