“…Some of the first applications of these vital fluors were in living animals, and in 1932 Singer studied kidney tissue of the frog, rat, and mouse subsequent to the injection of fluorescein. Pick and Zukerkandl (1935) also investigated this technique. Other fluors, including acriflavine, primulin, rhodamine B, and thioflavin S, were employed by Keller (1949) with submerged animal tissues.…”