Der p 2.1 Peptide Abrogates House Dust Mites-Induced Asthma Features in Mice and Humanized Mice by Inhibiting DC-Mediated T Cell Polarization

Klein, Martin; Colas, Luc; Cheminant, Marie‐Aude; Brosseau, Carole; Sauzeau, Vincent; Magnan, A.; Bouchaud, Grégory

doi:10.3389/fimmu.2020.565431

Cited by 7 publications

(3 citation statements)

References 53 publications

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“…However, given that the observed increases in DC abundance intratumorally and from BM-derived DCs are comparable, we are confident that the findings from bmDCs would translate to intratumor DCs. Additionally, bmDCs have previously been used to deduce specific aspects of DC biology using similar culturing conditions to that of this study (109)(110)(111)(112)(113), further supporting the validity of this methodology. Interestingly, through our in vivo and ex vivo studies we observed that L-fuc increases cDC1 and moDC abundance in similar proportions.…”

Section: Discussionsupporting

confidence: 69%

The functional role of L-fucose on dendritic cell function and polarization

Burton,

Bitaraf,

Snyder

et al. 2024

Front. Immunol.

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Despite significant advances in the development and refinement of immunotherapies administered to combat cancer over the past decades, a number of barriers continue to limit their efficacy. One significant clinical barrier is the inability to mount initial immune responses towards the tumor. As dendritic cells are central initiators of immune responses in the body, the elucidation of mechanisms that can be therapeutically leveraged to enhance their functions to drive anti-tumor immune responses is urgently needed. Here, we report that the dietary sugar L-fucose can be used to enhance the immunostimulatory activity of dendritic cells (DCs). L-fucose polarizes immature myeloid cells towards specific DC subsets, specifically cDC1 and moDC subsets. In vitro, L-fucose treatment enhances antigen uptake and processing of DCs. Furthermore, our data suggests that L-fucose-treated DCs increase stimulation of T cell populations. Consistent with our functional assays, single-cell RNA sequencing of intratumoral DCs from melanoma- and breast tumor-bearing mice confirmed transcriptional regulation and antigen processing as pathways that are significantly altered by dietary L-fucose. Together, this study provides the first evidence of the ability of L-fucose to bolster DC functionality and provides rational to further investigate how L-fucose can be used to leverage DC function in order to enhance current immunotherapy.

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Section: Discussionsupporting

confidence: 69%

The functional role of L-fucose on dendritic cell function and polarization

Burton,

Bitaraf,

Snyder

et al. 2024

Front. Immunol.

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“…Mice were housed in a ventilated cage system. Mice were sensitized on days 0, 7, 14, and 21 percutaneously and then challenged intranasally with Dermatophagoides farinae (Der f, Stallergenes Greer, Antony, France) on days 28, 29, 30, 35, 36, and 37 as previously described 23 . Mice were sacrificed on day 38 for analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Mice were sensitized on days 0, 7, 14, and 21 percutaneously and then challenged intranasally with Dermatophagoides farinae (Der f, Stallergenes Greer, Antony, France) on days 28, 29, 30, 35, 36, and 37 as previously described. 23 Mice were sacrificed on day 38 for analysis. Food allergy was modeled with three-week-old female BALB/cJRj mice (Janvier Laboratories, Le Genest-Saint-Isle, France) housed in a ventilated cage system and in specific pathogen-free conditions.…”

Section: Mouse Modelsmentioning

confidence: 99%

Engineering a safe monoclonal anti‐human IL‐2 that is effective in a murine model of food allergy and asthma

Klein

Misme-Aucouturier

Cheminant

et al. 2021

Allergy

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Background: Regulatory T cells (Tregs) are known to protect against allergies.Moreover, the decrease in the frequency and efficiency of Tregs amplifies allergic symptoms.Aim: This study investigated whether expanding Tregs in vivo with an IL-2/IL-2 antibody complex could be safe, well tolerated and efficient in a therapeutic setting in allergies. Methods:We produced an anti-IL-2 antibody (1C6) and demonstrated that when it is complexed to human IL-2, it increases IL-2 efficiency to induce Tregs in vivo without any detectable side effects. Furthermore, the IL-2/1C6 complex induces an increase in Helios expression by Tregs, suggesting that it not only elevated Treg numbers but also boosted their functions. Using mouse models of house-dust-mite-induced airway inflammation and wheat-gliadin-induced food allergies, we investigated the therapeutic potential of the IL-2/1C6 complex in allergies.Results: IL-2/1C6 treatment significantly reduced allergic symptoms, specific IgE production, the adaptive immune response and tissue damage. Interestingly, IL-2/1C6 treatment modulated innate lymphoid cells by increasing ILC2s in asthma and decreasing ILC3s in food allergies. Conclusion:In conclusion,complexed IL-2/anti-IL-2 may restore Treg numbers and function in respiratory and food allergies, thereby improving allergic markers and symptoms. Our IL-2/anti-IL-2 complex offers new hope for reestablishing immune tolerance in patients with allergies.

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Vaccination contre les allergies : immunothérapie spécifique des allergènes

Magnan

2024

Bulletin de l'Académie Nationale de Médecine

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Der p 2.1 Peptide Abrogates House Dust Mites-Induced Asthma Features in Mice and Humanized Mice by Inhibiting DC-Mediated T Cell Polarization

Cited by 7 publications

References 53 publications

The functional role of L-fucose on dendritic cell function and polarization

The functional role of L-fucose on dendritic cell function and polarization

Engineering a safe monoclonal anti‐human IL‐2 that is effective in a murine model of food allergy and asthma

Vaccination contre les allergies : immunothérapie spécifique des allergènes

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