Derivation of human decidua-like cells from amnion and menstrual blood

Sugawara, K.; Hamatani, Toshio; Yamada, Mitsutoshi; Ogawa, Seiji; Kamijo, Shintaro; Kuji, Naoaki; Akutsu, Hidenori; Miyado, Kenji; Yoshimura, Yasunori; Umezawa, Akihiro

doi:10.1038/srep04599

Cited by 26 publications

(25 citation statements)

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“…Cultured menstrual blood cells have been redifferentiated into the decidual cells. 42 Most research on menstrual blood cells has focused on their broad differentiation capacity into ectodermal, mesodermal, and endodermal lineages and their potential use in cell-based therapies. 43…”

Section: Menstrual Blood Mesenchymal Stem/stromal Cell-like Cellsmentioning

confidence: 99%

Stem Cells in Endometrial Physiology

et al. 2015

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Human endometrial mucosa is a dynamically remodeling tissue, undergoing cyclical morphologic and functional changes in response to fluctuating sex steroid hormones each menstrual cycle during a woman's reproductive life. Postmenopausal endometrium responds similarly to exogenous estrogen. Cyclical endometrial regeneration also occurs in nonmenstruating rodents, although to a lesser extent. The recent identification of rare populations of endogenous epithelial progenitor cells, mesenchymal stem/stromal cells (MSCs), the side population (SP) cells, and label-retaining cells (LRCs) suggests these stem/progenitor cell populations may play a key role in endometrial regeneration during menstrual and estrus cycles. This review summarizes the identification of epithelial progenitors, MSC, SP, and LRC, and discusses their contribution to endometrial tissue regeneration, maintaining tissue homeostasis, decidualization, and placentation. Markers for human endometrial MSC have been identified, revealing their perivascular location in both the functionalis and basalis layers. These markers also allow their purification from biopsy tissue and menstrual blood. These findings have advanced our understanding of normal endometrial physiology and will provide new insight into endometrial proliferative disorders (endometriosis, endometrial cancer). The ability to prospectively isolate endometrial MSC will enable their utilization in cell-based therapies for reproductive tract pathologies.

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Section: Menstrual Blood Mesenchymal Stem/stromal Cell-like Cellsmentioning

confidence: 99%

Stem Cells in Endometrial Physiology

et al. 2015

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“…Human menstrual blood-derived stem cells (MenSCs) are human menstrual blood progenitor cells (MBPCs) that are isolated from menstrual fluids [4–6]. MenSCs are similar to mesenchymal stem cells (MSCs), which have proliferative capabilities and broad multipotency, including the ability to differentiate into cell types belonging to all three germ lineages [6–8].…”

Section: Introductionmentioning

confidence: 99%

“…MenSCs are similar to mesenchymal stem cells (MSCs), which have proliferative capabilities and broad multipotency, including the ability to differentiate into cell types belonging to all three germ lineages [6–8]. MenSCs exhibit higher proliferation rates than bone marrow-derived MSCs and can be easily obtained without invasive procedures [9–11].…”

Section: Introductionmentioning

confidence: 99%

Exosomes derived from human menstrual blood-derived stem cells alleviate fulminant hepatic failure

et al. 2017

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BackgroundHuman menstrual blood-derived stem cells (MenSCs) are a novel source of MSCs that provide the advantage of being easy to collect and isolate. Exosomes contain some mRNAs and adhesion molecules that can potentially impact cellular and animal physiology. This study aimed to investigate the therapeutic potential of MenSC-derived exosomes (MenSC-Ex) on AML12 cells (in vitro) and D-GalN/LPS-induced FHF mice (in vivo).MethodsTransmission electron microscopy and Western blot were used to identify MenSC-Ex. Antibody array was used to examine cytokine levels on MenSC-Ex. MenSC-Ex were treated in D-GalN/LPS-induced AML12 in vitro. Cell proliferation and apoptosis were measured. MenSC-Ex were injected into the tail veins of mice 24 h before treatment with D-GalN/LPS. Blood and liver tissues served as physiological and biochemical indexes. The number of liver mononuclear cells (MNCs) and the amount of the active apoptotic protein caspase-3 were determined to elaborate the mechanism of hepatoprotective activity.ResultsHuman menstrual blood-derived stem cell-derived exosomes (MenSC-Ex) are bi-lipid membrane vesicles that have a round, ball-like shape with a diameter of approximately 30–100 nm. Cytokine arrays have shown that MenSC-Ex expressed cytokines, including ICAM-1, angiopoietin-2, Axl, angiogenin, IGFBP-6, osteoprotegerin, IL-6, and IL-8. MenSC-Ex markedly improved liver function, enhanced survival rates, and inhibited liver cell apoptosis at 6 h after transplantation. MenSC-Ex migrated to sites of injury and to AML12 cells (a mouse hepatocyte cell line), respectively. Moreover, MenSC-Ex reduced the number of liver mononuclear cells (MNCs) and the amount of the active apoptotic protein caspase-3 in injured livers.ConclusionsIn conclusion, our results provide preliminary evidence for the anti-apoptotic capacity of MenSC-Ex in FHF and suggest that MenSC-Ex may be an alternative therapeutic approach to treat FHF.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-016-0453-6) contains supplementary material, which is available to authorized users.

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“…Epithelial cells were not ordinarily sighted in cultured menstrual blood, because they were not present, were overlooked or had been coated by the stromal fibroblast populations [27] suggesting that epithelial progenitors are more likely located in the basalis and not normally shed in menstruation stage [26]. Cultured menstrual blood cells have been redifferentiated into the decidual cells [28]. Research on menstrual blood cells has mostly focused on the broad differentiation ability into endodermal, mesodermal, and ectodermal lineages and their potential use in cell-based therapies [29].…”

Section: Discussionmentioning

confidence: 99%

Stem Cells in Endometrium and Regeneration

Bahar¹

2019

SJBR

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Objective:The endometrium plays a key role in the fertility and endometrial pathology and gains great importance in the female reproductive system. It is important in terms of fertility and endometrial pathology with its mechanism and regulatory factors. Aim: This study highlights the complex process of endometrial regeneration and the researchs aimed at explaining the ability of endometrial stem cells to new remedy to the treatment of infertility as well as some diseases. Conclusion:There is no doubt that the endometrium has unlimited ability to regenerate any tissue. Today, they are in the various stages of experimentation in cell-based therapies of the regenerative medicine and will open hopeful avenues in treating heavy or fatal diseases by using stem cells. Beside endometrial diseases and infertility, an extensive range of clinical practices have included tests of stem cells in the endometrium. Furthermore, this study summarizes the identification of stem cells and regeneration properties in the endometrium.

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Derivation of human decidua-like cells from amnion and menstrual blood

Cited by 26 publications

References 50 publications

Stem Cells in Endometrial Physiology

Stem Cells in Endometrial Physiology

Exosomes derived from human menstrual blood-derived stem cells alleviate fulminant hepatic failure

Stem Cells in Endometrium and Regeneration

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